Performance specifications pass criteria
After the run, the software calculates average copy number values and standard deviation values.
The instrument passes performance specifications if the following inequality is true and the instrument
successfully distinguishes between unknown populations A and B with a statistical confidence level
of 99.7%.
[(C
tA
) – 3(σ
CtA
)] > [(C
tB
) + 3(σ
CtB
)]
• C
tA
= Average C
t
of unknown population A
• σ
CtA
= Standard deviation of unknown population A
• C
tB
= Average C
t
of unknown population B
• σ
CtB
= Standard deviation of unknown population B
The software automatically adjusts the threshold and omits a defined number of wells from the
unknown populations to meet the performance specifications. To view any omitted wells, open the
EDS file for the verification in the desktop software.
Prepare an RNase P plate
Materials required for RNase P plate preparation
• RNase P instrument verification plate
• Centrifuge with plate adapter; buckets cleaned before use
• Powder‐free gloves
• Safety glasses
Thaw, vortex, and centrifuge an RNase P plate
IMPORTANT!
Expose the RNase P plate to room temperature for no more than 45 minutes, inclusive
of thawing and preparation time.
After thawing, the RNase P plate cannot be refrozen.
1.
Remove the RNase P plate from the freezer, then thaw the plate in its packaging.
• Thaw the plate for approximately 5 minutes.
• Use the plate within 30 minutes of thawing.
2.
Confirm that the bench, vortex, and centrifuge are clean. Before use, wipe the vortex and
centrifuge using a lint
‑
free tissue.
3.
While wearing powder‐free gloves, remove the plate from its packaging.
4.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 ×
g
for 2 minutes.
Chapter 5
Calibrate and verify instrument performance
Perform instrument verification using RNase P plates
5
62
QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
