Prepare a calibration plate
Materials required for calibration plate preparation
• Plate(s) for the calibration that you are performing:
– ROI plate (the same plate is used for ROI and uniformity calibrations)
– Background calibration plate
– Dye calibration plates
Note:
We recommend calibrating with all Spectral Dye Calibrations Plates available for your
block configuration even if you are not using all the dyes in the plates.
Note:
Do not discard the packaging for the calibration plates. Each calibration plate can be used
up to 3 times if the following conditions are met:
·
The plate is stored in its packing sleeve at –15 to –25°C.
·
The plate is used within 6 months after opening.
·
The plate is used before the plate expiry date
• Centrifuge with plate adapter; buckets cleaned before use
• Powder‐free gloves
• Safety glasses
Thaw, vortex, and centrifuge a calibration plate
1.
Remove the calibration plate from the freezer, then thaw the plate in its packaging. Keep plates
protected from light until you perform the calibration.
• Thaw each plate for 30 minutes.
• Use each plate within 2 hours of thawing.
IMPORTANT!
Do not remove the plate from its packaging until you are ready to use it. The
fluorescent dyes in the wells of calibration plates are photosensitive. Prolonged exposure to light
can diminish the fluorescence of the dyes.
2.
While wearing powder‐free gloves, remove the calibration plate from its packaging and retain the
packaging. Do not remove the optical film.
3.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 ×
g
for 2 minutes.
4.
Confirm that the liquid in each well is at the bottom of the well and free of bubbles. If it is not,
centrifuge the plate again.
Chapter 5
Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations
5
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QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
