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Thermo Scientific TZ-32 Zonal Rotor

 General Description — 39

 

5. 4. 

Operational Checkpoints

5. 4. 1.  Setting of sample temperature and rotor temperature

Make the temperature of injecting sample and density gradient solution and the setting temperature of a rotor when 

spinning as equal as possible. If not, following problems may occur:

1.  When the temperature of injecting solution is lower than the temperature of the spinning rotor:

Because of the temperature rise of the solution injected into the rotor, the volume expands and the pressure inside 

the rotor increases.The push button of the cap assembly cannot be pushed down and the cap cannot be removed.

As the result, it is impossible to unload. Following table shows the quantities of increased volumes when using the 

TZ-32 rotor and water as sample.

Temperature Rise

Increase of Volume

5 °C 

➞

 15 °C, 10 K

1.5 ml

5 °C 

➞

 25 °C, 20 K

5.0 ml

Countermeasure

While operating at 3000 rpm, cool the rotor to the temperature when the solution is injected to the rotor. Then try to 

remove the cap again. If it is still difficult, stop running the rotor and push down the push button hard, then remove 

the cap.

2.  When the temperature of Injecting solution is higher than the temperature of the spinning rotor:

Because of the temperature fall of the solution injected into the rotor the volume decreases and the inside of the rotor 

becomes a negative pressure status. The cylinder of the cap assembly and the pushbutton are sucked up to the rotor 

shaft. As the result, the cap cannot be removed. The decreases of volumes are the same values of the increases of 

volumes shown in the above table.

Countermeasure

Remove the cap while operating at 3000 rpm. If still difficult, rise the temperature of the rotor to the temperature 

when the solution is injected while operating at 3000 rpm and try to remove the cap again.

Precautions on mounting the seal assembly Wipe the spinning Rulon seal surface with a tissue paper moistened 

with water lightly just before mounting the seal assembly. This prevents the Rulon seal from wearing abnormally and 

makes the cap removal easier Keep the seal assembly as horizontal as possible and center it visually. Lower the seal 

assembly vertically onto the Rulon seal surface and mount it quickly taking care not to hit the adapter on the Rulon 

seal surface. Never incline the seal assembly when mounting. If the seal assembly is mounted in inclined condition, 

the tip of the adapter made of Delrin may hit the Rulon surface and the Rulon may be faulty. The flow resistance of 

extraction pipe must not be large. The diameter of the extraction pipe must not be small and the length must not be 

long. The flow cell of the UV monitor must not be a micro flow cell .

5. 4. 2.  Precautions on loading and unloading

1.  Do not feed bubbles into the rotor at loading

 

»

In the pipe connection, provide bubble eliminating equipment to the position just before the seal

 

»

Be sure to clamp the pipe to keep the no-bubble status up to the exit of the contact body of the seal 

assembly on all occasions and setting is carried out.

Table 8

Increase of Volume

Содержание TZ-32 Zonal Rotor

Страница 1: ...Thermo Scientific TZ 32 Zonal Rotor for the Thermo Scientific WX Centrifuge Instruction Manual 50146926 a 01 2015 ...

Страница 2: ...g and Unloading 11 4 Assembly 12 4 1 Rotor Assembly 12 4 1 3 Rotor body and cover 12 4 1 4 Septa 12 4 1 5 Shaft 13 4 1 6 Stopper 13 4 1 7 Rulon seal 13 4 1 8 Cap assembly 13 4 2 RPZ S Seal Attachment Assembly 14 4 2 9 Seal assembly 14 4 2 10 Guard plate assembly 16 4 2 11 Shield plate 16 5 Operation 17 5 1 General Description 20 5 2 Preparation 21 5 2 1 Prepare the Centrifuge 21 5 2 2 Connect a tu...

Страница 3: ...pacity in Rotor 39 5 6 Separation Characteristic 39 5 7 Rotor Life 39 6 Maintenance 40 6 1 Cleaning of Rotor and Septa 40 6 2 Sterilization of Rotor 40 6 3 Inspection of Rotors 41 6 4 Handling of Rulon Seal and Renewal 42 6 5 Disassembling and Cleaning of Cap Assembly 43 6 6 Disassembling and Cleaning of Seal Assembly 43 6 7 Cleaning of Ball Bearing 44 6 8 Consumable Parts of the Rotor 45 6 9 Serv...

Страница 4: ...in detail 30 Setting the seal packing 31 Assembling the Rulon seal and O ring on the shaft 31 Shaft into septa 32 Assembled septa into rotor 32 Assemble the rotor cover 33 Mount the guard plate 33 Mount the seal assembly 34 Mount the shield plate 35 Feed a gradient solution by pump 35 Injection of sample and overlay 36 How to avoid bubbles while injecting a density gradient solution and sample 36 ...

Страница 5: ...es 10 Technical Specifications 12 Employed materials 20 Chemical Resistance of Noryl 21 Separation Procedure 22 Operation modes 33 Increase of Volume 39 Separation characteristic 41 Rotor Life 41 Sterilization methods 43 Troubleshooting 49 Defective phenomena and corrective action sealed sections 50 ...

Страница 6: ...ity of dynamic continuous loading unloading and analytical operation of samples while the rotor is spinning Because of the high performance and reliability the TZ 32 Zonal rotor can be used in many fields of laboratory work and commercial production 1 1 Intended Use The rotor is used in combination with the according centrifuge as a laboratory product designed to separate components by generation ...

Страница 7: ...nts Rotor cover Rulon seal 439345 Metal ring 451152 Seal packing 451153 Cap assembly 327251A Stopper 455897 Shaft 209280 Septa 103547 CAUTION The TZ 32 zonal rotor assembly does not include the RPZ S seal attachments assembly It has to be ordered seperately See Figure 2 on page 7 Figure 1 Rotor Components ...

Страница 8: ...B 2401 For rotor 2 O ring 8062014 1AP14 JIS B 2401 For Rulon seal 2 O ring 8062003 1AP3 JIS B 2401 For Rulon seal 2 O ring 8062018 1AP18 JIS B 2401 For shaft 6 O ring 8062218 1AS18 JIS B 2401 For shaft 2 Stopper 455897 2 Instruction manual 50146926 1 Over speed decal 34644414 2 Screwdriver 464912 1 Tool box 320717 1 Circlip remover 84850103 1 Lubricant for screws 84810201 1 Table 1 Rotor Accessori...

Страница 9: ...2 Seal Attachment Assembly Seal Attachment Components Part 1 of the shield plate assembly 322897B Guard plate assembly 104230A Part 2 of the shield plate assembly 323896B Seal assembly 217428A Figure 2 Seal attachment components Type RPZ S Part cord 901306 ...

Страница 10: ...6 6 D6 flat washer 80202088 D6 6 Tee connector 484286 4 Rubber tube clamp 466544 6 Tygon tube 662905 R 3603 1 8 x 1 4 x 5 m 1 Ball bearing 60000178 1 Support S408766 3 Spacer S408765 3 Extractor 479965 For Rulon seal 1 Seal rubber S202661 1 Snap ring remover 84850109 1 Hexagon bar wrench 60000122 For M4 setscrew 1 Holder base 343920A 1 Handle 323663A 1 Table 2 Seal Attachment Accessories ...

Страница 11: ...for swing rotor immediately stop using it and contact the nearest agent of Thermo Fisher Scientific When operation is over store the rotor after cleaning and drying Make it a practice to keep a logbook for rotors Keeping a logbook for rotors is indispensable for controlling the life or when the warranty clause is involved Keep the logbook up to date and store it properly Do not rotate this rotor a...

Страница 12: ...hnical Specifications Maximum Speed 32000 rpm Maximum Centrifugal Force 102000 x g K factor 363 Capacity 1690 ml Maximum Density of Gradient Solution 1 2 g ml Maximum Radius 8 89 cm Rotor Material Titanium Alloy Weight 15 4 kg Table 3 Technical Specifications ...

Страница 13: ...peed Rotation Cap Cover of rotor 3 2 Loading and Unloading Pipe for cooling water Pipe in the rotor center Pipe on the outer wall of rotor Seal assembly Guard plate Shaft Rotor Over speed decal Septa Figure 3 Construction High Speed Rotation Figure 4 Construction Loading and Unloading ...

Страница 14: ... The septa has four vanes that divide the bowl into four sector shaped compartments each vane containing a channel to carry fluid to the outer edge of the rotor bowl The solution injected through the seal assembly is led to the outer edge of the rotor from the four holes located outside the Rulon seal through the shaft The septa has slanting surfaces the top of each being provided with a hole comm...

Страница 15: ...lled and material is Noryl 4 1 7 Rulon seal The Rulon seal mouted on the top of shaft This part is important component in loading and unloading and material is fluorine plastic 4 1 8 Cap assembly The cap is mounted onto the shaft with the seal assembly removed after injecting a sample into the rotor It seals the sample in the rotor when evacuating the rotor chamber and spinning the rotor at a high...

Страница 16: ...njected through the pipe is introduced through the body and Rulon seal into the rotor being spinned One of the two higher pipes projecting from the body which is located on the outer wall side of the rotor is led to the outer groove of the Rulon seal and the outer wall of the rotor from the four septa vanes through the four holes of the Rulon seal The pipe in the rotor center is led to the four ho...

Страница 17: ...cooling water pipes Pipe in the rotor center Cooling water pipe Coil spring B Body Seal Assembly Ball bearing Adaptor Cover Shaft 11 Rotor body 11 Rulon seal 12 Holder cover 13 Guide body 14 Pipe 15 Pipe on the outer wall side of rotor Figure 10 Construction of the seal assembly ...

Страница 18: ...ls etc from dropping into the rotor chamber while it is spinning Case 4 2 11 Shield plate It is mounted on top of the rotor chamber to suppress frosting in the chamber when injecting gradient solution or samples into or extracting them from the rotor which is being cooled Since the shield plate is also made of a transparent acrylic resin plate the rotor chamber is clearly visible through this plat...

Страница 19: ...the over speed decal and rotor body Replacing over speed decal To replace the over speed decal follow this procedure 1 Preparation Prepare a new over speed decal and knife Wash and then dry the rotor well 2 Hold the rotor upside down 3 Pry the edge of the over speed decal with the knife and remove the decal Be very careful not to damage the rotor 4 Clean the decal hole on the rotor by using alcoho...

Страница 20: ...rotor according to the following equation Employed Materials The following materials are used on the passage of the sample Part name Material Tygon tube PVC specially processed Tee Polypropylene Metallic section of seal attachment assembly etc Stainless steel Stationary seal surface seal assembly side Tungsten carbide Rulon seal Tetrafluorethylene resin Rulon O Ring NBR Rotor cover shaft metal rin...

Страница 21: ...ium nitrate Ammonium sulfate Lactic acid Potassium sulfate Amyl acetate Nitric acid Sodium bicarbonate Amyl alcohol Ortho phosphoric acid Sodium bisulfate Borax Picric acid Sodium carbonate Bromine Phosphoric acid Sodium chlorate Butyl alcohol Stearic acid Sodium cyanide Butyl cellosolve Sulfuric acid Sodium hydroxide Butyl mercaptan Ferrous sulfate Sodium nitrate Benzoic acid Formaldehyde Sodium ...

Страница 22: ...dge of the rotor The gradient solution containing sedimented particles is unloaded by feeding a higher density solution at the edge of the rotor Also the absorbancy of the solution thus unloaded is fractioned by a fraction collector while it is being measured by a spectrophotometer In a common use prepare the density gradient in the order of high to low density layers from the center to the edge o...

Страница 23: ...2 Preparation 5 2 1 Prepare the Centrifuge Mount the supports a Mount the three supports onto the fixed pieces in the rotor chamber of the centrifuge as shown in Fig 5 1 b Center and adjust height of guard plate Support Fixed piece Figure 14 Mounting the supports ...

Страница 24: ...le on the support erecting the right knob and then turning it to horizontal Fixed block Base Case Settler Knob Shaft Handle Adjusting the base a Loosen the four upper screws in the fixed block to make it movable Then loosen the lock nut of the setter Move the fixed block and setter back and forth by turning the setter until the acryl plate is aligned with the rotor center Tighten the screws to fix...

Страница 25: ... with that of the base Tighten the screws and fix the base Adjust the case while visual checking until it is concentrical with at the center of the shaft Case Shaft Height adjustment of case Adjust the height of the rotor and case as shown in Figure 18 Case Guard plate Shaft Rotor Spinning Shaft Figure 17 Adjusting the base Figure 18 Height adjustment ...

Страница 26: ...s follows Loosen the three setscrews by the hex bar wrench accessory to this rotor and the case can be turned Turning clockwise lowers it while turning counterclockwise lifts it After adjustment tighten the three setscrews to fix the case Case Guard plate Figure 19 Tightening the three setscrews ...

Страница 27: ...raction from the rotor The outer one is led to the outer wall of the rotor and the center one is led to the center of the rotor The two remaining pipes are used for cooling gradient solution and samples when necessary Use pipes with an inner diameter of 3 2 millimeter 1 8 Pump Tee Cooling water Pipe on the rotor center Cooling pipes lower at the top end than the other two pipes Pipe on the outer w...

Страница 28: ... rotor speed to 3000 rpm b Mount the seal assembly with the tubes connected c Inject about 100 ml of distilled water by the pump or injector through the pipe leading to the outer wall of the rotor Leakage from the seal assembly if any can be detected from much splashed injected solution sticking to the rotor chamber of the centrifuge If any leakage is detected check as follows before performing th...

Страница 29: ...ect If they are set incorrect the seal packing comes out of the seal assembly thus possibly causing the leakage of solution and damaging the rotor CAUTION Since O ring and seal packing which are used in the rotor or the connecting portion of the seal section will come into contact with sample always rinse them before use When these parts are replaced some residue generated when rubber was formed m...

Страница 30: ...tch the Rulon seal surface by a fingernail When inserting the seal carefully push it with the soft portion of finger applied Apply a thin coat of vacuum grease to the O ring Rulon seal O Ring 1AP14 O Ring 1AP3 O Ring 1AS18 Stopper O Ring 1AP18 Shaft 3 Put the shaft into the septa Figure 22 Setting the seal packing Figure 23 Assembling the Rulon seal and O ring on the shaft ...

Страница 31: ...ta into the rotor Fit the hole in the bottom end of the shaft onto the extrusion on the rotor bottom where the O ring 1AS22 is inserted Thus the upper surfaces of both rotor and septa will be flush with each other Shaft Septa Rotor Figure 24 Shaft into septa Figure 25 Assembled septa into rotor ...

Страница 32: ...ch mark on the cover will go over that on the rotor body which however will result in no trouble Handle Holder base Match marks 5 3 2 Preparation for spinning the rotor 1 Mount the rotor on the spinning shaft in the rotor chamber of the centrifuge 2 Mount the guard plate Secure the fixed piece on the left support first and fix it by turning over the right hand support toward the right support For ...

Страница 33: ... operation mode from panel 2 Set operating factors for high speed spinning 3 Press the START switch 1 Close the door 2 Press the VACUUM switch 3 Press the START switch 1 When the timer counts down to 0 the rotor decelerates to 3000 rpm 2 Press the VACUUM switch NOTE When it is desired to stop the rotor set to the normal operation mode on the panel For details on operating from the panel refer to t...

Страница 34: ...ect one end to the pump and the other one to the clamp b Pipe on the rotor center Put one end of the pipe into a beaker containing water Make sure gradient solution is infected Clamp the other end CAUTION at the time of infecting gradient solution Select such on infection speed of gradient solution that infection pressure of the pump pipepressure at infecting side of the seal is below 1 kg cm Spec...

Страница 35: ...olution has filled the tee clamp both ends of the pipe and stop the pump Inject a sample through one end by an injector Then feed an overlay of 75 ml into the rotor CAUTION Do not feed bubbles while injecting a density gradient solution and sample Otherwise the gradient solution and sample will be disturbed If bubbles are about to enter lead them to another tube from the tee by setting upright the...

Страница 36: ...oval of air bubbles a Pipe on the outer wall of the rotor Clamp the pipe on the pump side b Pipe on the rotor center Prepare about 15 ml of an overlay disconnect the clamp on the rotor side and clamp the other end for injection Bubbles will be removed through the pipe on the rotor center 7 Remove the shield plate and remove the seal assembly Remove the tubes from the tube setter and then the shiel...

Страница 37: ... completely Push the fringe 3 For the high speed spinning see 5 3 3 Spinning on page 33 5 3 6 Deceleration and extraction 1 Regulation of the rotor speed to 3000 rpm by deceleration see 5 3 3 Spinning on page 33 2 Push the R PUMP button and stop the vacuum pump to let air into the rotor chamber 3 Remove the cap Hold the running cap by both hands Pull it upwards while pushing the center extrusion 4...

Страница 38: ...onal Rotor General Description 38 gradient solution Clamp other end a Pipe on the rotor center Clamp one end Lead the other end to a fraction collector through a spectrophotometer flow cell Figure 37 Deceleration and extraction ...

Страница 39: ...on are sucked up to the rotor shaft As the result the cap cannot be removed The decreases of volumes are the same values of the increases of volumes shown in the above table Countermeasure Remove the cap while operating at 3000 rpm If still difficult rise the temperature of the rotor to the temperature when the solution is injected while operating at 3000 rpm and try to remove the cap again Precau...

Страница 40: ... layer in the pipe for eliminating bubbles to absorb the shock of flow against the seal assembly 3 Control the pressure for injecting density gradient solution The higher the concentration of solution the slower the injection speed It is permitted to measure the injection pressure with a pressure gauge to keep the injection pressure at 1 kg cm or less 4 Precautions on mounting the seal assembly Wi...

Страница 41: ...Keep the rotor operation log regurlarly The rotor operation log is necessary for control of the rotor life Operations Time 1000 2500 h While using the rotor repeatedly its strength decreases gradually due to fatigue and creep of material by which the rotor life is determined Sum up the number of operations and number of operating hours and if either value reaches the figures primary life shown in ...

Страница 42: ...40 50 C after use and wipe off water with a soft cloth After fully drying it coat it with stopcock grease Immediately after a corrosive sample was used immerse the rotor in neutral detergent excluding those containing chlorine diluted with lukewarm water and rinse it sufficiently in distilled water Removal of metal ring Remove it with the accessory screwdriver or the like which has a thin flat end...

Страница 43: ...nm Gas Ethylene oxide Formaldehyde Chemical solution Ethanol 70 Hydrogen peroxide 3 Formalin 6 3 Inspection of Rotors Check the rotor as follows after every use If the optical adapter disk is unclean wipe it with a soft cloth but do not damage it Replacements of packings and O rings are available If the rotor appears to be corroded deformation cracks or discoloration is observed do not use the rot...

Страница 44: ...cover and polish the contact surface until it becomes even Do this carefully while removing polishing agent from the Rulon seal surface and sandpaper Finish it after making sure no scratch due to the sandpaper remains on the Rulon seal surface Removal of Rulon seal The Rulon seal can be washed and sterilized together with the shaft Remove the Rulon seal if the Rulon seal is scratched Be careful no...

Страница 45: ...f it has too much play due to wear of ring Replace the cap with new one if the corner that catches the stopper is not square Push button Handle body Cylinder Ring Washer Snap ring Cylinder case 6 6 Disassembling and Cleaning of Seal Assembly Disassemble the seal assembly by the following procedure See Figure 44 on page 46 1 Loosen screws and detach the holder cover 2 Loosen screws and remove the s...

Страница 46: ...e other in the ball bearing Fully wash it in water or lukewarm water use and dry it sufficiently with an air blower or dryer Slightly apply aluminum screw lubricant After drying to the balls to the contacts of the balls with between the inner and outer rings Apply a small amount of lubricant to the inner and outer rings at three positions or so and turn the bearing so that the lubricant can be app...

Страница 47: ...O Ring Cap assembly Seal packing Stopper 6 9 Service Parts List for the Cap Assembly No Item Quantity Article No Handle body 1 456310 Cylinder case assembly 1 456132A Ring 2 458785 Cylinder 1 456331 Push button assembly 1 456320A Washer 1 450477 Snap ring 1 450483 Figure 45 Service parts list for the cap assembly ...

Страница 48: ...178 3 Circlip 1 481840 6 Housing 1 437842 7 Ring 1 437844 8 Spring 1 437845 9 Spring holder 1 437846 10 M5 x 10 flat head screw 2 80155598 11 Circlip 1 4650213 16 Contact body assembly 1 439825A 17 Guide body 1 437863 18 Coil spring 1 452958 20 M5 x 12 cross recessed pan head screw 2 80152408 21 M5 spring washer 2 80112058 Figure 46 Service parts list for the seal assembly ...

Страница 49: ... on page 39 Pressure drops abruptly during loading and unloading The sample injection line is bypassed See Table 6 on page 39 Sample leakage and vibration of seal assembly 1 The installation condition of the guard plate 2 The condition of the seal assembly 1 Level the guard plate Rattle the three supports Center the rotor and guard plate See 5 2 Preparation on page 23 Adjust the height of rotor an...

Страница 50: ...njury 2 Poor sealing due to insufficient grease Apply grease 7 1 Check the Rulon Seal There are most problem occurrences of Rulon seal in the defect in the operation of zonal rotor Explain the point of the check of Rulon seal Check whether the Rulon seal is faulty or not Scratches or dents on surface Abnormal wear on surface due to previous sample leakage Is the height of the surface of the outsid...

Страница 51: ...rners of the stopper for wear to prevent the cap from falling off Replace the stopper with new one every six to twelve months Check of other O rings mounted to the shaft for scratch Be careful when inserting the shaft into the septa because the O rings are susceptible to scratches and cuts Before inserting apply a thin coat of vacuum grease to the shaft and O ring for smooth insertion Check of sam...

Страница 52: ...Inc and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details United States Canada 1 866 984 3766 Latin America 1 866 984 3766 Austria 43 1 801 40 0 Belgium 32 53 73 42 41 France 33 2 2803 2180 Germany national toll free 0800 1 536 376 Germany international 49 61 84 90 6000 ...

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