Thermo Scientific TZ-32 Zonal Rotor
General Description — 28
Cooling water
Cooling water is needed for cooling the contact body, which touches the RuIon seal. Use city water as coolant in case
centrifuge is to be done at normal temperature. However use cooled water when centrifuge is to be done at a temperature
below 5 °C. The minimum flow rate required is 450 ml/min.
5. 2. 3. Confirm test operation
Check the seal assembly for leakage before injecting a gradient solution into the rotor. This check should be done by
injecting distilled water into the rotor by the pump or the injector. The operation and handling are the same as in injecting
gradient solution.
Check the seal assembly in the following procedure.
a. Set the rotor and guard plate, and regulate the rotor speed to 3000 rpm.
b. Mount the seal assembly with the tubes connected.
c. Inject about 100 ml of distilled water by the pump or injector through the pipe leading to the outer wall of the
rotor.
Leakage from the seal assembly, if any, can be detected from much splashed injected solution sticking to the rotor
chamber of the centrifuge. If any leakage is detected, check as follows before performing the preliminary operation again.
Refer to item 7 for the detailed check.
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Check that the Rulon seal is mounted onto the shaft normally.
(Check that the O-ring is mounted or if there is any scratch on the top surface.)
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Check that the seal assembly body operates smoothly.
(Check the contact between the Rulon seal and contact body of seal assembly, and also the spring which
depresses the guide body.)
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Check if the outlet pipe on the rotor center blocks normal flow of solution.
For repair of the Rulon seal surface,
