Thermo Scientific TSQ Quantum XLS, Руководство пользователя

Страница: 127 / 176

6
Maintenance
Removing and Installing a GC Capillary Column
Thermo Scientific TSQ Quantum XLS Series User Guide 111
c. Wearing clean, lint- and powder-free gloves, unwind about one turn of the capillary
column (shown in Figure 61 on page 108 ) from the column outlet end.
d. Wipe about 450 mm (18 in.) of the column with a tissue soaked in methanol.
e. Insert the column through the transfer line nut and ferrule. Wipe the column again
with a tissue soaked in methanol.
f. Score and break the end of the column with a scoring wafer. With the magnifying
glass, check for an even, flat cut. Repeat if necessary.
g. Insert the column into the transfer line:
i. Open the lid of the ion source vacuum chamber so that you can get a better view
of the column.
ii. Using the I/R tool, remove the ion volume. See “Removing the Ion Volume” on
page 73 .
iii. Insert the column into the transfer line and tighten the transfer line nut by hand.
iv. Push the column in until you can see it through the inlet valve.
v. Pull the column back just far enough so that you cannot see it.
vi. Tighten the transfer line nut and transfer line union.
vii. Using the I/R tool, replace the ion volume. See “Installing the Ion Volume” on
page 80 .
8. Condition the transfer line ferrule. Graphite/Vespel™ ferrules like the transfer line ferrule
require conditioning to ensure a leak-tight seal.
a. Raise the oven temperature to the maximum temperature for operating the column
(normally 300 °C).
b. Wait 10 minutes.
c. Lower the oven temperature to 30 °C and allow it to cool before continuing.
d. Retighten the transfer line nut and the transfer line union.
9. Set up the gas chromatograph:
a. Make sure the column does not have any sharp bends and that it does not touch any
metal objects or walls inside the oven.
Note Sliding a septum on the column before the transfer line nut helps you
measure the proper distance between the nut and the end of the column. Ensure
that the column extends approximately 1 to 2 mm past the end of the transfer
line.
WARNING Burn Hazard .The oven might be hot. Allow it to cool to room
temperature before opening it. Do not touch the injector, which will still be hot.