6
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Calibration of the Instrument for Measurement Analysis
Section
Section
Thermo Scientific
Orion 2295 Phosphate Analyzer
|
39
To edit the reagent volume, from the MAIN MENU follow the sequence:
MAIN MENU
➔
SETUP
➔
METHOD
➔
(+) to go to page 2
➔
STIR TIME (1, 2, 3)
During the addition of reagent, the stirrer is on and the info field (if enabled) shows the reagent
pumping progress, with a bar and the volume pumped. After adding the reagent, the countdown
of stir time is also shown along with the absorbance of the sample mixture.
Collect Data
In collect data, the final reading is recorded and the final absorbance is displayed at the end of
the cycle.
The stability of the recorded reading depends on the specified stability criteria. You have the
choice of editing the stability criteria. To do this from the MAIN MENU follow the sequence:
MENU
➔
SETUP
➔
ANALYSIS PARAMETERS
➔
STABILITY CRITERIA
Now the concentration is calculated (as follows) and the current concentration is updated.
Concentration = (Slope x Absorbance) + Intercept
(Where slope and intercept are from the CURRENT calibrated values of the instrument for
measurement.)
Post Wash
The programmable POST WASH is to wash the reaction cell after the measurement and
evacuate any residue of the reaction mixed solution. This event is optional and can be disabled,
or the reaction cell can be washed with selectable sources with editable volumes and stir times,
with up to five programmable steps. To perform post wash after measurement, select ANALYSIS
PARAMETERS for the POST WASH EVENT.
To set the post wash parameters from the MAIN MENU follow the sequence:
MAIN MENU
➔
SETUP
➔
ANALYSIS PARAMETERS
➔
(+) POST WASH EVENT
➔
POST
WASH CYCLES
NOTE:
If the pump is not assigned to a reagent, it will abort the measurement and
the info field will flash with the error message: “PUMP IS NOT ASSIGNED”.
NOTE:
If unstable and doesn't meet the stability criteria, the info field will flash with
the warning message “COLLECTING DATA, UNSTABLE SIGNAL”.
