Bubble Trap
To manage air in the system, the CTS
™
Rotea
™
Single-Use Kit includes a small bubble trap with a
capacity of approximately 5 mL.
1
2
Figure 9 CTS
™
Rotea
™
Single-Use Kit and close-up of Bubble Trap
1
CTS
™
Rotea
™
Single-Use Kit
2
Close-up of Bubble Trap
CAUTION!
Once the Bubble Trap volume is consumed, any additional air in the system will now
be able to enter the CFC Chamber and ultimately cause an over-pressure fault, shutting down the
instrument.
CAUTION!
Protocols created for the Rotea
™
instrument must include a suitable priming sequence
to ensure air in fluid supply lines and the Bubble Trap is replaced with fluid prior to commencing
processing.
Note:
If the length of tubing from the product supply bag has a volume greater than the Bubble Trap,
then air can enter the circuit. It is therefore preferred that the length of tubing for each connection (1/8”
ID assumed) is less than 12” (or 300 mm). In some cases where the supply vessel is large, e.g. remote
bioreactor, additional process strategies can be included to re-fill the supply tube when needed.
Chapter 3
Basic instrument operation
Kit preparation, loading and removal
3
CTS
™
Rotea
™
Counterflow Centrifugation System User Guide
47
Содержание A44769
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