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4-16KR

Use as a robot-controlled Centrifuge

Version 10/2010, Rev. 1.9 of 02/07/2012

Translation of the original operating manual

6.3.3.5

Hardware interface (optional accessory)

Pinning of the connector

Pinning of the 9 pin SUB-D (male) connector on the cover of the
centrifuge

PIN 2: TxD (transmit)

PIN 3: RxD (receive)

PIN 5: GND (ground)

PIN 1,4,6,7,8,9: not connected

Typical connection to a PC

Typical connection to a personal computer or a terminal with serial
RS232 interface:

9-pin male socket at PC / terminal:

Standard 1:1 serial cable (9 pin female

9 pin female)

Required: PIN 2 PIN 2

TxD (Cent.) RxD (PC)

PIN 3

RxD (Cent.) TxD (PC)

PIN 5

GND (Cent.)

GND (PC)

25-pin male socket at PC / terminal:

Standard 1:1 serial cable (9 pin female

9 pin female)

+ 9-25 way Adaptor, 9 pin male 25 pin female

Serial cable (9 pin female

25 pin female):

Pin-Pin Configuration (only boldface printed required):

D Sub 9 : 1

6 7 8 9

D Sub 25 : 8

6 4 5 22

Do not use a null modem cable/adapter with crossed RxD/TxD
signals!

Содержание 4-16KR

Страница 1: ...ersion 10 2010 Rev 1 9 of 02 07 2012 Translation of the original operating manual Operating Manual Refrigerated Centrifuge for Robot Technology 4 16KR from serial no 128012 Please retain for later use...

Страница 2: ......

Страница 3: ...by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Phone 49 0 55 22 50 07 0 Fax 49 0 55 22 50 07 12 E mail info sigma zentrifugen de For service please use our servic...

Страница 4: ......

Страница 5: ...nsibility of the Operator 15 3 4 Operating Personnel 15 3 5 Informal Safety Instructions 15 3 6 Safety Instructions 16 3 6 1 Electrical Safety 16 3 6 2 Mechanical Safety 16 3 6 3 Safety Instructions f...

Страница 6: ...Accessories 24 6 1 3 1 Installation of the Rotor 24 6 1 3 2 Installation of Accessories 25 6 2 2 1 Adapters 26 6 1 3 3 Tubes 26 6 2 Spincontrol Universal Manual Operation 27 6 2 1 Operating Panel 27...

Страница 7: ...fuges with motor driven lid or hatch 50 Commands for centrifuges with rotor positioning 50 Commands for Rotor Cycle Counter 50 6 3 3 5 Hardware interface optional accessory 51 Pinning of the connector...

Страница 8: ...sal of the Packaging 63 10 Technical Data 64 10 1 Ambient Conditions 64 11 Appendix 65 11 1 Suitable Accessories 65 11 2 Grafical Representation of the Rotors 65 11 3 Formulae Mathematical Relations 6...

Страница 9: ...driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any other use beyond this area of application...

Страница 10: ...parts Non compliance may be prosecuted under criminal law 1 5 Standards and Regulations EC Declaration of Conformity page 71 1 6 Scope of Supply The centrifuge comprises 1 rotor wrench size 13 Part no...

Страница 11: ...ation of the original operating manual 2 Design of the Centrifuge 2 1 Overview 1 Lid 2 Sliding cover 3 Operating panel 4 Mains switch Fig 2 1 Total view of the centrifuge 5 Name plate see page 12 Fig...

Страница 12: ...rer and registered office 2 Type name 3 Serial number 4 Max speed 5 Kinetic energy 6 Max density 7 Nominal voltage 8 Input fuse 9 Symbol for special disposal see chapter 9 10 CE mark in accordance wit...

Страница 13: ...Ein Netzverbindung On Power Marche mise sous tension O Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschluss Protective earth ground Liaison la terre Erde Earth ground Terre Netzs...

Страница 14: ...endangering injuries This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious health problems up to life endan...

Страница 15: ...s intended see chapter 1 2 Intended Use page 9 of this Operating Manual take measures for the safe opening of centrifuges 3 4 Operating Personnel Persons operating the unit must be familiar with the f...

Страница 16: ...when the rotor is in motion Do not use the centrifuge if it was installed incorrectly Do not use the centrifuge without panels Do not use the centrifuge if the rotors and inserts show signs of corros...

Страница 17: ...e Prevention Always use fuses with the same type and rating specified Do not spin explosive or inflammable substances Do not use the centrifuge within hazardous locations 3 6 5 Chemical and Biological...

Страница 18: ...ve the instructions on the installation of accessories see 6 1 3 2 The rotor must be loaded symmetrically at equal weights If liquids with a density 1 2 g cm3 are used reduce the speed see 11 3 2 Dens...

Страница 19: ...roperly closed The electrical lock must be locked The lid can only be opened when the rotor has stopped If the lid is opened by way of the emergency release system during operation the centrifuge will...

Страница 20: ...ll be switched off in the acceleration phase or during the run The message Imbalance and the error number 46 for Imbalance will then flash on the display The lid opens automatically after the rotor ha...

Страница 21: ...in dry rooms The storage temperature must be above 20 C If you would like to store it for more than one year or if you intend to ship it overseas please contact the manufacturer 4 3 Notes on Transpor...

Страница 22: ...rox 134 kg Retain the packaging for any possible future transport of the centrifuge 5 2 Transport Safety Device The transport safety device consists of two plastic screws which are located at the bott...

Страница 23: ...even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before the cent...

Страница 24: ...is opened To close press with both hands slightly on the lid until the electrical locks are locked Do not hold your fingers between the lid and the housing when closing the lid Risk of crushing A flas...

Страница 25: ...four buckets of the swing out rotor need to be installed when spinning Always load the opposite inserts buckets of the rotors with the same accessories and fill to avoid imbalance Centrifugation with...

Страница 26: ...ubes Load the tubes outside of the centrifuge Liquids in the buckets or multiple carriers cause corrosion Fill the tubes carefully and arrange them according to their weight Imbalances result in the e...

Страница 27: ...e 3 Lid key 4 Stop key 5 Function keys Fig 6 4 Operating panel The centrifuge is started directly via the operating panel When the centrifuge is switched on all of the operating keys and displays will...

Страница 28: ...own on the display The stop key can be used to cyclically select various combinations Activating the softstart function Activating the softstart function and the softstop function Activating the softs...

Страница 29: ...nstead of in steps of 10 seconds in the min sec mode or in steps of 1 minute instead of in steps of 10 minutes in the hrs min mode Keep the stop key pressed while setting the desired runtime with the...

Страница 30: ...er minute rpm and depend on the RCF values see 11 3 Formulae Mathematical Relations The maximum speed values depend on the rotor that is used When the maximum permissible speed of the rotor is reached...

Страница 31: ...the desired value is displayed Press the right function key to confirm the selected value If this is not done the value will be automatically reset to the last setting It is also possible to change th...

Страница 32: ...erature is above the actual temperature it will be represented by one single bar on the temperature scale Fig 6 13 Fig 6 13 Set temperature above actual temperature If the set temperature and the actu...

Страница 33: ...the corresponding RCF value The message cont for contiuous run and the symbol will be displayed in the time field Start the centrifuge by pressing the start key Fig 6 14 Rapid cooling program RAPID_TE...

Страница 34: ...Program Mode The program is used to save or load certain recurrent settings of the centrifuge 50 different programs can be saved and called up This is only possible at a standstill Attention Temperatu...

Страница 35: ...be adopted Fig 6 17 Calling up a stored program here program number 08 6 2 5 Lockdown This function locks the parameters against all inadvertent changes Turn the left function key until a padlock sym...

Страница 36: ...ctivating Deactivating the Sound Signal If this function is active a sound signal can be heard at the end of the operation as well as in the event of an error message or imbalance Press the lid key fi...

Страница 37: ...commands trigger an immediate action Change of input values Display of current process values Display of current input values Change of parameters Display of parameters Indication of the centrifuge s...

Страница 38: ...n case sensitive The character received won t be echoed by the centrifuge processor normally You can tell the centrifuge to echo each character by sending the echoon command The user command and the r...

Страница 39: ...ss values speed or IN_PV_1 requests the actual rotor speed temp or IN_PV_2 requests the actual temperature time or IN_PV_3 requests the remaining time Commands to request setpoints getsetspeed or IN_S...

Страница 40: ...rred status1 advanced status of the centrifuge The value is displayed hexadecimal Bit Status Normal centrifuge or with motor driven lid Centrifuge with hatch in the lid 1 0 00 Door is opening closing...

Страница 41: ...22 0 0020 cmderror displays the error status of the last command The centrifuge returns 1 if no error occurred syserror displays the error status of the centrifuge The centrifuge returns 0 if no error...

Страница 42: ...d Return Message Description Ok Command successful CNF Command not found NEA Not enough arguments e g set speed value missing ERR Command not possible WD Watchdog reset occured echooff This command de...

Страница 43: ...etcurve 22 CurveNr 22 Interval 1 Time 130 Speed 112 LIN Interval 2 Time 60 Speed 148 Interval 3 Time 60 Speed 193 Interval 4 Time 60 Speed 256 Interval 5 Time 60 Speed 326 Interval 6 Time 60 Speed 524...

Страница 44: ...did not start Still running Still running Interrupted by error xy Error during run Speed was partly out of setting Speed error detected by run observation Stopped by user Stop button pressed Not start...

Страница 45: ...run Still running Still spinning OK Run time OK Run Time from Set Speed Run time from set speed Used Only if normal run started and Item was used Changed during run Item was changed during run OK Item...

Страница 46: ...g Heating if started ab degree Fahrenheit Celsius Output depends on set temperature and set temperature unit Not Reached Set temperature was not reached Not reached yet Set temperature still not reach...

Страница 47: ...p Fast stop Curve x Normal run output depends on set deceleration curve Changed during run Set deceleration curve was changed during run Still running Still spinning OK Set deceleration curve unchange...

Страница 48: ...time IN_PV_3 0 1 sec UINT getsetspeed IN_SP_1 0 1 rpm UINT getsettemp IN_SP_2 0 1 C INT getsettime IN_SP_3 0 1 sec UINT setaccel OUT_PAR_1 1 0 acc curve nr UINT setdecel OUT_PAR_2 1 0 dec curve nr UI...

Страница 49: ...ettemp 5 CR LF settime 120 CR LF start CR LF requesting the actual status of the centrifuge status CR LF answer string 0 CR LF rotor is spinning or 1 CR LF rotor is stationary requesting all actual va...

Страница 50: ...atically during positioning pos CR Outputs the position of the rotor in positioning mode run n CR Starts the centrifuge with speed n rpm If the centrifuge is equipped with a hatch in the lid this comm...

Страница 51: ...nection to a personal computer or a terminal with serial RS232 interface 9 pin male socket at PC terminal Standard 1 1 serial cable 9 pin female 9 pin female Required PIN 2 PIN 2 TxD Cent RxD PC PIN 3...

Страница 52: ...is locked mechanically The slide cover in the lid opens during the locking of the rotor either automatically depending on the input command or separately following another command During loading or un...

Страница 53: ...in contact service Centrifuge cannot be started Lid key LED flashes The lid lock is not closed correctly Open and close lid If the error occurs again contact service Centrifuge decelerates during oper...

Страница 54: ...he opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole The key will be guided through a funnel shaped tube to the shaft of the lid lock motor Fig 7...

Страница 55: ...9 Lid error press lid key close lid remove foreign matter from the opening of the lid lock device With error 50 and 51 the centrifuge will stop 60 69 Process error allow to slow down power off on With...

Страница 56: ...e state the type of your centrifuge and its serial number Make use of our service request on the Internet Please use the request form see above on our website 8 Maintenance and Service The centrifuge...

Страница 57: ...minated with toxic radioactive or pathogenic substances clean the rotor chamber immediately with a suitable decontamination agent depending on the type of contamination Take suitable precautions for y...

Страница 58: ...ic radioactive or pathogenic contamination Dry the accessories with a soft cloth or in a drying chamber at approx 50 C 8 1 2 1 Plastic Accessories The chemical resistance of plastic decreases with ris...

Страница 59: ...ss particles e g with a vacuum cleaner Replace the rubber cushions since even thorough cleaning will not remove all glass particles Glass particles will damage the surface coating e g anodizing of the...

Страница 60: ...or drugstores The centrifuges and the accessories consist of various materials A possible incompatibility must be considered Before using cleaning or decontamination agents that were not recommended b...

Страница 61: ...polyallomer and polycarbonate 115 118 30 40 Fig 8 1 Autoclaving table 8 3 Service In the event of service work that requires the removal of the panels there is a risk of electric shock or mechanical...

Страница 62: ...ugh we exercise great care during the production of our products it may be necessary to return a unit or accessory to the manufacturer In order to ensure the quick and economical processing of returns...

Страница 63: ...charge If an order is placed these charges will be offset The unit must be packaged in a transport safe manner Please use the original packaging if at all possible If the product is dispatched to us...

Страница 64: ...5 66 897 Other parameters Time range Temperature range Storage locations short run continuous run 10 sec 11 h 59 min 20 to 40 C 50 Physical data Height mm Width mm Depth mm Weight kg EMC as per EN 613...

Страница 65: ...r Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 8213 Fax 49 0 55 22 50 07 92 13 E mail info sigma zentrifugen de Rotors and accessories of the standard centrifuge 4 16K must not be used...

Страница 66: ...he equation If then the speed or the rotation radius is changed the resulting RCF will be recalculated If the RCF is altered the speed will be adapted accordingly under the consideration of the radius...

Страница 67: ...4 16KR Appendix Version 10 2010 Rev 1 9 of 02 07 2012 67 Translation of the original operating manual Fig 11 2 Speed Grravitational Field Diagram...

Страница 68: ...100 3 4 4 1 4 4 2 1 1 Antimony trichloride SbCl3 90 1 4 1 4 1 1 1 4 Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 1 Benzene C6H6 100 3 2 4 1 3 4 4 1 1 Boric acid H3BO3 aqueous 1 3 1 2 1 1 1 Butyl acrylate...

Страница 69: ...ogen chloride HCl 5 1 4 1 4 1 1 1 1 4 Hydrogen chloride HCl concen trated 1 4 4 4 1 1 2 3 1 4 Hydrogen peroxide H2O2 3 1 3 1 1 1 1 1 1 3 Hydrogen peroxide H2O2 30 1 4 1 4 1 1 1 1 3 Hydrogen sulfide H2...

Страница 70: ...ate AgNO3 100 1 1 1 1 1 1 1 1 1 4 Sodium bisulfite NaHSO3 10 1 1 2 4 1 1 1 Sodium carbonate Na2CO3 10 1 1 1 1 1 1 3 Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1...

Страница 71: ...EC Declaration of Conformity...

Страница 72: ......

Страница 73: ...peration 53 Centrifuge display 24 27 Centrifuge is inclined 53 Centrifuge was moved during run 53 Centrifuges definition 9 Changes in color 60 Changing the RCF Increments 31 Changing the speed Increme...

Страница 74: ...ife and health 14 Disinfectants 60 Disinfection of the rotor chamber and accessories 60 Disposal of the centrifuge 63 Disposal of the packaging 63 Documentation 10 Drive problem 53 EC Declaration of C...

Страница 75: ...18 24 Installation of the rotor 24 Installation site 23 Instructions on saftety and hazards 26 Intended use 9 Interrupting a centrifugation run 28 Interrupting a deceleration process 28 IP Code 64 Le...

Страница 76: ...ck symbol 35 Parameter 30 31 34 37 66 Parameter error 55 Pathogenic substances 17 57 58 60 Permanent lockdown 35 Pinning of the connector 51 Pivot bearing 59 Plastic accessories 58 Possible error caus...

Страница 77: ...Safety distance 18 23 Safety instructions 9 25 Safety instructions for centrifugation 18 Safety Instructions for robot controlled centrifuges 17 Safety conscious work 15 Saving the current settings 3...

Страница 78: ...System error 53 55 Table of rotors and accessories with a different service life 19 Table of user commands 48 Technical data 64 Temperature 31 Temperature error 55 Temperature fuses 23 Temperature pr...

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