QIAamplifier 96 User Manual 03/2020
54
The currently activated step is highlighted in blue in the table above the input fields (see Figure 21).
Note
: By navigating between program steps, it is not necessary to confirm the settings for each
single parameter or step and to leave the screen for editing program steps. Instead, you can fill in
the parameters for all steps subsequently and confirm your entries after all steps are programmed.
8.6.1.1
Programming loops
A typical PCR program consists of repetitive steps for denaturation, annealing, and extension. For
the repetition of steps, the QIAamplifier 96 software enables the programming of loops. For a loop
two parameters have to be set:
1.
The step number to which the program should return (
Go To
)
2.
The number of repetitions (
Cycles
)
To program a loop, select the last step of the loop and enter the step number to which the program
should return in the
Go To
field (see Figure 21). To define the number of repetitions, enter a number
in the Cycles field (see Figure 21).
For example, to program the loop for the following protocol, select step
4
and enter the value
2
in
the
Go To
field and the value
35
in the
Cycles
field.
Step
Temp.
Time
Cycles
1
94 °C
05:00
2
94 °C
01:00
3
55 °C
01:00
35x
4
72 °C
01:00
5
72 °C
05:00
6
16 °C
Pause
In this example, the device first executes steps 1 to 4, then repeats step 2 to step 4 for 34 times,
performs the final extension at 72°C, and holds the temperature indefinitely at 16°C in the last step.
Содержание QIAamplifier 96
Страница 21: ...QIAamplifier 96 User Manual 03 2020 21 Figure 1 Voltage selector switch on the underside of the device...
Страница 38: ...QIAamplifier 96 User Manual 03 2020 38 Figure 9 QIAamplifier 96 enter password screen...
Страница 41: ...QIAamplifier 96 User Manual 03 2020 41 Button Block status Color Free Green Running Yellow Pause Blue...
Страница 99: ...QIAamplifier 96 User Manual 03 2020 99 Figure 65 QIAamplifier 96 Cycler Info Screen...
Страница 120: ...QIAamplifier 96 User Manual 03 2020 120 Document Revision History Date Changes March 2020 Initial release...