Qiagen QIAamplifier 96, Руководство пользователя

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QIAamplifier 96 User Manual 03/2020 104

To reduce or increase the hold time from step to step within a loop, press ∆t and set a time
increment.

To adjust the heating rate, press ∆R and enter a value between 0.1°C and max. You can
accept the heating rate for all steps in the program by checking the Apply heating/cooling
rate to all steps box.

To program a gradient, press Grad . On the next screen, you can choose between standard
Std. and linear gradient programming Lin. . For standard programming, enter a temperature
for both the first and last row in the sample block. For linear programming, enter the primer
annealing temperature and set a temperature increment. The gradient curve is displayed
graphically. Press

to confirm your entries.

Press

on the screen for editing program steps to accept the settings. The software leaves the
screen and opens the spreadsheet or graphical program display.

Alternatively, individual program parameters for a step can also be edited. To do this, in the
spreadsheet view press directly on the cell for the parameter in the program table which you
want to edit. In the graphical view press on the desired parameter edit field. Program options
(loops, temperature, and time increment, as well as heating and cooling rate) must be
activated using the button to the left of the graphical display.

To insert a program step, select a step and press Insert . The screen for editing program steps
opens. Enter the desired parameters and confirm the settings by pressing

.

To delete a program step, select it and press Delete .
Incubation at 15°C rather than 4°C at the end of a run increases the device’s life time.

When all the parameters for all steps have been entered, confirm the settings on the
programming screen with
Done . The software opens the screen for copying and storing
programs.

To save the program, select a user directory and a storage location. Then, press Save . The
program is now saved.