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RamanFlex 400 Series Getting Started Guide . 3 

Table of Contents 

Introduction ............................................................................................... 5

 

About This Manual ............................................................................................ 6

 

Conventions Used in this Manual ........................................................................ 7

 

Notes, Cautions and Warnings ..................................................................... 7

 

Safety Practices ....................................................................................... 11

 

Overview .........................................................................................................12

 

General Operating Conditions............................................................................13

 

Environmental Conditions .................................................................................14

 

Fire Safety .......................................................................................................15

 

Electrical Safety ...............................................................................................16

 

Electrical Protection ...................................................................................16

 

Power Supplies ..........................................................................................17

 

Power Cords (Between PSU and Power Outlet Socket) .................................18

 

EMC Compliance ..............................................................................................19

 

Laser Safety .....................................................................................................20

 

Class 3B Safety Precautions ........................................................................21

 

General Laser Safety Issues .......................................................................21

 

Internal Interlock System ...........................................................................24

 

External Interlock System ..........................................................................24

 

Warning Labels ................................................................................................25

 

Labels on the Exterior of the RamanFlex 400/400F ......................................25

 

Fuses ..............................................................................................................26

 

Raman History, Theory and Instrumentation .......................................... 27

 

The History of Raman Spectroscopy ..................................................................28

 

Raman Theory .................................................................................................29

 

The RamanFlex 400 Series ................................................................................31

 

Spectrograph Detector Systems ..................................................................32

 

Preparing your Facility ............................................................................. 35

 

Specifications ...................................................................................................36

 

PC Requirements ..............................................................................................37

 

Hardware Requirements .............................................................................37

 

Software Requirements ..............................................................................37

 

Windows Administrator Level .....................................................................37

 

Finding a Suitable Location for the Instrument ...................................................38

 

Instrument Dimensions .....................................................................................39

 

Installing your RamanFlex 400 ................................................................ 41

 

Unpacking the Instrument ................................................................................42

 

Lifting the Instrument .......................................................................................43

 

Attaching the External Interlock Override...........................................................44

 

Unpacking and Assembling the PC .....................................................................45

 

Switching on the Instrument .............................................................................46

 

Installing Spectrum Software ............................................................................47

 

Before you Start ........................................................................................47

 

Installation Procedure ................................................................................48

 

Moving the Instrument .....................................................................................60

 

Introduction to the RamanFlex 400 ........................................................ 61

 

Spectrometer Front Panel (Console) ..................................................................62

 

General Overview of the Fiber Optic Probe .........................................................63

 

Attaching and Removing the Fiber Optic Probe...................................................64

 

Attaching the FOP......................................................................................64

 

Removing the FOP .....................................................................................65

 

Acquiring Spectra with the Fiber Optic Probe .....................................................66

 

Sampling Solids .........................................................................................66

 

Sampling Liquids .......................................................................................66

 

Содержание RamanFlex 400

Страница 1: ...RamanFlex 400 Series Getting Started Guide MOLECULAR SPECTROSCOPY ...

Страница 2: ...t not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this p...

Страница 3: ... 28 Raman Theory 29 The RamanFlex 400 Series 31 Spectrograph Detector Systems 32 Preparing your Facility 35 Specifications 36 PC Requirements 37 Hardware Requirements 37 Software Requirements 37 Windows Administrator Level 37 Finding a Suitable Location for the Instrument 38 Instrument Dimensions 39 Installing your RamanFlex 400 41 Unpacking the Instrument 42 Lifting the Instrument 43 Attaching th...

Страница 4: ...bration Performance Verification 78 SOP 8 Wavelength Calibration Verification of the RamanFlex 400 80 SOP 9 Wavelength Calibration of the RamanFlex 400 81 SOP 10 Laser Frequency Calibration of the RamanFlex 400 82 SOP 11 Intensity Calibration of the RamanFlex 400 82 Summary 83 Additional Parts and Upgrades 84 Safety and Calibration 84 Fiber Optic Probes 84 Maintenance and Troubleshooting 85 Mainte...

Страница 5: ...Introduction ...

Страница 6: ...man spectroscopy Preparing your Facility This chapter shows you how to position your RamanFlex 400 and what utilities are required for example electricity temperature control Installing your RamanFlex 400 This chapter contains step by step instructions about how to unpack and assemble your RamanFlex 400 and how to install the software Introduction to the RamanFlex 400 This chapter gives an overvie...

Страница 7: ... have to press two keys at the same time for example ALT F All eight digit numbers are PerkinElmer part numbers unless stated otherwise The term RamanFlex 400 Series means the RamanFlex 400 and the RamanFlex 400F Raman Spectrometers Notes Cautions and Warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indicates additiona...

Страница 8: ... en otros En recuadros éste se proporciona información sobre este tipo de circunstancias Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves détériorations de l instrument ou d autre matériel Les détails sur ces circonstances figurent dans un encadré semblable à celui ci Caution Attenzione Con il termine CAUTION ATTENZIONE ve...

Страница 9: ...recuadros como éste se proporciona información sobre este tipo de circunstancias Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels à l utilisateur ou à d autres personnes Les détails sur ces circonstances sont données dans un encadré semblable à celui ci Warning Pericolo Con il termine WARNING PERICOLO vengono segnalate s...

Страница 10: ...10 RamanFlex 400 Series Getting Started Guide ...

Страница 11: ...Safety Practices ...

Страница 12: ...lieved to be correct and complete PerkinElmer shall not be liable for errors contained herein nor damages concerned with the use of this document PerkinElmer does not accept liability for the interpretation of information resulting either directly or indirectly from our spectrometers or associated software interfaces All results supplied by PerkinElmer s spectrometers or software are intended to p...

Страница 13: ...30 C Relative Humidity 75 maximum non condensing WARNING If the equipment will be used at temperatures exceeding 35 o C we recommend that you contact your PerkinElmer Service Representative and request a visit to optimize the instrument performance for such elevated temperatures Avoid any adjustment maintenance or repair of the opened operating instrument If any adjustment maintenance or repair of...

Страница 14: ...ltitude up to 2000 m Ambient temperatures of 5 C to 40 C A maximum ambient relative humidity of 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains fluctuations not exceeding 10 of the nominal voltage WARNING If the equipment is used in a manner not specified herein the protection provided by the equipment may be impaired ...

Страница 15: ... Safety Ensure that there is at least 15 cm 6 between any cooling vents and adjacent equipment or walls Ensure that the power supply unit PSU vents or fan do not become obstructed Figure 1 Figure 1 Power supply unit Fan Vents ...

Страница 16: ...7 Electrical Protection Insulation Class I rating for external circuits Only connect equipment that meets the requirements of IEC 61010 1 IEC 60950 or equivalent standards Installation Category The instruments are able to withstand transient overvoltages typically present on the MAINS supply The normal level of transient overvoltages is impulse withstand overvoltage category II of IEC 60364 4 443 ...

Страница 17: ...ARNING Under no circumstances should you attempt to disassemble the PSU and carry out repairs If the PSU has a fault please contact your PerkinElmer Service Engineer The entire unit must be replaced Connect the PSU to a three wire live neutral and ground earth socket The ground must be a non current carrying wire that is connected to the earth ground at the main distribution box The power point so...

Страница 18: ...ld be appropriate for the country listed as the shipping destination WARNING If the power cord is not appropriate for your country it must be removed from use immediately and replaced If the power cord has any physical damage it must be removed from use immediately and replaced To avoid electrical hazards the ground pin on the power cord should not be interfered with in any way ...

Страница 19: ...ith the EMC standard EN61326 EMC standard for electrical equipment for measurement control and laboratory use and EN55011 ISM class A rf emissions FCC rules and regulations This product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section...

Страница 20: ...authorized operation and contact PerkinElmer for advice before using the instrument again The RamanFlex 400 Series are classified as Class 3B The FOP provides a focused beam of laser radiation at 785 nm This type of laser radiation is classified as a Class 3B laser source During normal operation laser radiation is accessible from the end of the probe The output energy should be enclosed whenever p...

Страница 21: ...only operate Class 3B lasers in a designated area 2 You must fit each Class 3B device with a captive key control switch such that the key cannot be removed from the lock except in the OFF position The key should be removed from the laser when it is not in use and kept in a safe place in the custody of a nominated person Keys must never be issued to unauthorized persons 3 Each Class 3B device shoul...

Страница 22: ...sure The laser should also be turned off and the probe covered when the system is not in use AVOID DIRECT EXPOSURE TO THE BEAM Figure 2 Schematic of the fiber optic probe not to scale When using the fiber optic probe the following precautions must always be observed Use an interlock switch on the door to the laboratory and or the reaction vessel being used in association with the fiber optic probe...

Страница 23: ...ions When the probe is in use always ensure that no stray laser radiation is directed at people in the vicinity Use protective laser safety goggles with an optical density OD of at least 3 at 785 nm as a precaution against accidental exposure to the direct or reflected laser light Suitable goggles are available from PerkinElmer part number L1323518 Do not use the TFOP if it shows any sign of damag...

Страница 24: ...ard distance for 785 nm Raman systems Table 2 Nominal Ocular Hazard Distance NOHD for 785 nm Probe focal length mm NOHD for 100 mW exiting fiber optic probe cm 7 5 10 27 3 NOTE Other focal lengths are available contact PerkinElmer for details In particular the use of an ultra long working distance lens adaptor part number L1320071 provides a working distance of 25 mm Where different focal lengths ...

Страница 25: ...de modified power voltage and current values MAXIMUM OUTPUT 250mW PULSE DURATION CONTINUOUS WAVE EMITTED WAVELENGHS 785nm THIS LASER PRODUCT CONFORMS TO IEC 60825 1 1993 21 CFR 1040 10 1040 11 LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT MAXIMUM OUTPUT 250mW PULSE DURATION CONTINUOUS WAVE EMITTED WAVELENGHS 785nm THIS LASER PRODUCT CONFORMS TO IEC 60825 1 1993 21 CFR 1040 10 1040 ...

Страница 26: ...ential injury to yourself and damage to the instrument switch OFF all components in the system and disconnect them from the line power supply before you alter or make any new electrical connections The only fuses that you may have to replace are those in the mains power cords UK type plug only ...

Страница 27: ...Raman History Theory and Instrumentation ...

Страница 28: ... observed the scattering from water and alcohol visually In addition to the blue incident light a faint green light also scattered later named Stokes scattering By the 1930s much of the theory underlying Raman scattering was understood but due to the complexity of the instrumentation the initial interest in Raman spectroscopy was not maintained Improvements in charge coupled device CCD detectors f...

Страница 29: ...okes scattering or shorter wavelengths higher frequencies ν Anti Stokes scattering With Stokes scattering some of the incident photons give up a portion of energy to the molecules and are detected at a lower frequency longer wavelength than the Rayleigh radiation Figure 6 Figure 6 Rayleigh Stokes and Anti Stokes Scattering Wavenumber cm 1 O No change in energy no useful information Shift from exci...

Страница 30: ...otational energy of the molecule The frequency shifts from the frequency of the incident beam are displayed on the x axis of a Raman spectrum generally in relative wavenumbers otherwise known as Raman Shift and the intensity of the bands are displayed in arbitrary units on the y axis Figure 7 Raman spectrum of cyclohexane showing Stokes anti Stokes and Rayleigh scattering ...

Страница 31: ... is composed of a laser mirrors fiber optics filters lenses spectrograph and a CCD detector The laser supplies light through an objective lens onto the sample through the fiber optic probe The scattered light from the sample is collected with the same objective lens and transmitted back to the spectrometer Figure 9 Schematic of the RamanFlex 400 Series Laser Spectrograph Optical Head Objective Len...

Страница 32: ...tor The system works by dispersing the light in two dimensions by using two gratings Figure 10 The first grating disperses the light horizontally onto the second grating where the light is further dispersed vertically onto the CCD detector The light is spread over a number of strips on the CCD that are read simultaneously therefore a full spectral range at high resolution can be achieved with one ...

Страница 33: ... with a full spectral range There are no moving parts in the spectrograph which improves instrument reliability ruggedness and the quality of calibration The absence of moving parts also means that as you acquire the spectrum there is no change in the spectrograph s stray light performance meaning that there are no stitch marks in the final spectrum No light is lost all the light goes onto the det...

Страница 34: ...34 RamanFlex 400 Series Getting Started Guide ...

Страница 35: ...Preparing your Facility ...

Страница 36: ...re 15 C 59 F 30 C 86 F Recommended Temperature 20 C 68 F 22 C 72 F Power Supply 100 240 V 10 50 60 Hz If the electricity supply does not conform to these specifications please consult PerkinElmer prior to installation If the supply of power is erratic please use an uninterruptible power supply UPS Incorrect shutdown power fluctuations or brown outs may damage the spectrometer ...

Страница 37: ... able to create new data files CD ROM drive Ethernet network connection A keyboard and USB or PS 2 style mouse Hi Speed USB 2 0 port x2 Serial RS232 port Parallel port Software Requirements Spectrum requires that Windows XP Professional Service Pack 2 operating system is installed on the PC before you install the software We have specified Windows XP Professional because it is a robust industry st...

Страница 38: ...allow the user to work comfortably Use conducting floor coverings to minimize static discharge Floor vibrations or noise from heavy manufacturing equipment can affect the performance of the instrument Intense magnetic fields should be at least 5 5 meters 18 feet away from the spectrometer Never place the instrument near a window that gets direct sunlight We recommend that you install the instrumen...

Страница 39: ...hs approximately 20 kg Laboratory benching must be strong enough to support the combined weight of the instrument and any ancillary equipment without warping or wobbling The bench must be at least 650 mm 26 in depth Figure 12 Dimensions of the RamanFlex 400 Series 530 mm 600 mm 161 mm ...

Страница 40: ...40 RamanFlex 400 Series Getting Started Guide ...

Страница 41: ...Installing your RamanFlex 400 ...

Страница 42: ... LX108871 Spectrum Std for Raman Software Kit Contains the Spectrum Std Software CD LX100884 the Raman Instrument Configuration Disk LX108869 the Raman Instrument Installation Disk LX108925 and the RamanFlex 400 Series Getting Started Guide L1321840 Spectrum ES for Raman Software Kit Contains the Spectrum ES Software CD LX100881 the Raman Instrument Configuration Disk LX108869 the Raman Instrument...

Страница 43: ...Flex 400 spectrometer weighs 20 kg To avoid injury or damage to the instrument please use proper lifting techniques WARNING To lift and or move the instrument safely requires two people WARNING Always hold the instrument under the base when you lift the spectrometer ...

Страница 44: ...rlock must be satisfied You must connect an external door interlock to the 7 way DIN connector labeled INTERLOCK on the rear panel of the spectrometer or attach the External Interlock Override L1320264 supplied with the instrument If your Laser Safety Officer determines that an interlock is required on the door to the room you will need to purchase the Raman Laser Safety Kit L1320262 For more info...

Страница 45: ... from the PC and the monitor into the mains electrical supply and then switch on 2 If you are prompted to login to Windows when you start the PC log in If your PC was provided by PerkinElmer your username and password will be Username Administrator Password Administrator 3 Click OK You can now start using the PC For PC security we recommend that the Administrator Password is changed after installa...

Страница 46: ...se keys 2 If your instrument is fitted with a deep depletion CCD connect the 7 5 V PSU L1323525 from the DD POWER socket on the rear panel to a mains power socket 3 If your instrument is fitted with a deep depletion CCD connect the Tygon tubing between the water recirculator L1323010 and the WATER IN OUT sockets on the rear panel fill the storage tank of the cooler with water and then plug in and ...

Страница 47: ...anti virus software running on the PC The USB cables between the PC and the RamanFlex 400 and if appropriate between the PC and triggered fiber optic probe are NOT connected If the PC you intend to use already has a copy of Spectrum installed and is being used to control IR instruments you should follow the steps described below before you carry out the installation required to enable the PC to co...

Страница 48: ...art automatically from the Start Menu select Run The Run dialog is displayed 3 Enter d Setup exe and then click OK Replace d with the drive letter for your CD The InstallShield Wizard dialog appears while the system is preparing to install the software When the installer is ready the Welcome page is displayed If your PC does not meet the software requirements given in Software Requirements startin...

Страница 49: ...product s and then enter the License Number s as found on the Software License Certificate You must enter the correct license number s to proceed with the installation If you enter an incorrect license number the Next button is disabled The Spectrum Beer s Law check box is automatically selected when you select the Spectrum check box You do not need to enter a license number for Spectrum Beer s La...

Страница 50: ... Install the Program page is displayed 8 Click Install to begin installing Spectrum The Installing Spectrum Software page is displayed which informs you of the status of the installation When the installation is complete the InstallShield Wizard Completed page is displayed ...

Страница 51: ... If the installation program does not start automatically from the Start Menu select Run The Run dialog is displayed 12 Enter d setup exe and then click OK Replace d with the drive letter for your CD The InstallShield Wizard dialog appears while the system is preparing to install the software When the installer is ready the Welcome to the InstallShield Wizard for Raman Instrument page is displayed...

Страница 52: ...eries Getting Started Guide 13 Click Next The License Agreement page is displayed 14 Read the license and if you accept the terms select that option and then click Next The Ready to Install the Program page is displayed ...

Страница 53: ...an Instrument page is displayed which informs you of the status of the installation During this installation the DirectX Setup page is displayed 16 Read the license and if you accept the terms click Yes The Installing Raman Instrument page is displayed again which informs you of the status of the installation ...

Страница 54: ...ge 18 Click Start The Enter Instrument Details page is displayed 19 In the Instrument Alias field enter a name for your Raman instrument This should be a unique name to identify this instrument 20 In the Serial Number field enter the serial number of your instrument This is displayed on the rear panel of the instrument ...

Страница 55: ...for the PerkinElmer software that is already installed on the PC The default User name and Password are administrator The Locate Instrument Configuration Files page is displayed 22 Place your Raman Instrument Configuration Disk into your CD drive wait a few seconds and then click Copy Configuration Files If your configuration files have already been installed on your PC click Browse and navigate t...

Страница 56: ... the rear panel of the spectrometer module to the PC Figure 13 USB 2 0 cable NOTE If you disconnect one of the USB cables and reattach it to a different USB port then you will be prompted for the drivers By default the drivers are stored in C Program Files PerkinElmer ServiceIR Raman 24 Wait until the Welcome to the Found New Hardware Wizard is displayed ...

Страница 57: ...s helping you install software for USB Device In this case continue with the installation in the same way as for IDUS 26 Select Install from a list or specific location Advanced and then click Next The Please choose your search and installation options page is displayed 27 Select Include this location in the search click Browse and then navigate to C Program Files PerkinElmer ServiceIR Raman ...

Страница 58: ... 28 Click Next The Completing the Found New Hardware Wizard is displayed 29 Click Finish The Found New Hardware Wizard closes 30 On the Install PerkinElmer Raman Instrument page click Continue The Instrument Installation Complete page is displayed ...

Страница 59: ...m standby and Turn off hard disks to Never This completes the installation of the Spectrum software If appropriate you should now install the triggered fiber optic probe drivers as described in the Raman Triggered Fiber Optic Probe leaflet L1321887 and then connect the fiber optic cables from the triggered fiber optic probe to the RamanFlex 400 as described for a standard fiber probe in Attaching ...

Страница 60: ...Two people are needed to lift the instrument If you need to ship your instrument you must use the original supplied packing materials to prevent damage to the system When the instrument has reached its destination you should perform the calibration routines described in Calibration and Performance Verification SOPs on page 78 ...

Страница 61: ...Introduction to the RamanFlex 400 ...

Страница 62: ... instrument is receiving power Laser This LED indicates the power status of the laser If the LED is illuminated then the laser is receiving power This does not mean that laser radiation is present throughout the optical path of the instrument only that the laser power is on Active This LED indicates the status of the laser shutters If the LED is illuminated then the shutters are activated open and...

Страница 63: ...ing on page 41 The Fiber Optic Probe FOP is designed to enable the remote measurement of samples It is available in a range of different configurations to suit your application However it should be noted that compatibility of the working environment with the probe should be verified with PerkinElmer prior to use The probe body contains the filtering optics and is interfaced with two fiber optic ca...

Страница 64: ... to the rear panel 4 Remove the retained screw in caps from the two couplers at the bottom left of the panel If you have a RamanFlex 400F and wish to connect a second fiber optic probe remove the retained screw in caps from the two couplers below the LED FIBER 2 connector 5 Connect the excitation cable of the FOP to the coupler marked EXC Make sure that the polarizing ridge on the FOP cable is ali...

Страница 65: ...o the rear panel 4 Remove the plug in the socket marked LED FIBER 1 or LED FIBER 2 if you are removing the second FOP from your RamanFlex 400F 5 Carefully remove the retained ends of the FOP by unscrewing and pulling the fiber optic cables away from the couplers perpendicularly Be extremely careful when removing the FOP as you can easily damage the instrument 6 Replace the plastic caps on the ends...

Страница 66: ...r infrared region Room lights and sunlight will contaminate your spectra Take care not to misinterpret room lights or sunlight features as Raman features Sampling Liquids Liquid samples can be analyzed by immersing the FOP in the liquid of interest or by analyzing through the container Immersion The FOP can only be immersed in liquid if you have purchased an immersible fiber probe sleeve Do not to...

Страница 67: ...lass fluorescence is a problem and it is not possible to use quartz containers then it is possible to acquire a spectrum of the container and spectrally subtract this from any spectra containing this fluorescent signature Sampling Slurries Highly absorbing species Please refer to Sampling Liquids For sample immersion care taken in focusing the probe within the sleeve can result in a dramatic impro...

Страница 68: ...68 RamanFlex 400 Series Getting Started Guide ...

Страница 69: ...Using the RamanFlex 400 ...

Страница 70: ...mber of tasks The procedures provided may not be the most straightforward or simple for the task you wish to perform so treat these Standard Operating Procedures SOPs as a starting point and with time you may wish to develop your own SOPs which better suit your specific mode of use This chapter also acts as a learn by doing section allowing you to explore the functionality of the software and spec...

Страница 71: ...s about calibrating the spectrometer see Calibration and Performance Verification SOPs SOP 1 Switching the System On 1 Switch on the PC and monitor 2 Login to Windows 3 Ensure the laser power key is in the ARM position on the front panel of the instrument 4 Ensure the main power supply is connected to a mains outlet and that the outlet is switched on 5 To turn on the spectrometer press the PSU pow...

Страница 72: ...n but switch the PC off overnight if required 1 Shut down Spectrum software by selecting Exit from the File menu to return to the Windows operating environment 2 Switch off the spectrometer by pressing the power switch on the PSU to OFF ON Ο OFF SOP 3 Setting Up and Saving Experimental Parameters This SOP is designed to act as a quick guide to help you start collecting data The exact setup for opt...

Страница 73: ...d like to save this set of parameters as the default set that loads when the software is opened then save your experiment settings as Default rex If you select Save from the File menu the current experimental parameters are stored as the default parameters in the software NOTE The Spectrum software automatically saves to the currently loaded REX file without prompting any configuration changes mad...

Страница 74: ...Number of scans and a minimum of 1 Background Handling Renew background at start of each sample Experiment page Advanced tab Yes Sampling Area Experiment page Sampling tab Select the number and style of sampling points Sampling Type Experiment page Sampling tab If applicable select the spacing and number of points Accessory Configuration Beam path Instrument page Hardware tab Fiber Optic Probe Ram...

Страница 75: ...which the scan data will be stored NOTE If you choose to save spectra to a folder other than the default one specified for your instrument the data will not be displayed automatically To view the spectrum you will need to open the data file manually using the Open option from the File menu after the scan has completed 5 Click Scan NOTE If you have come to this screen via the Setup Scan Parameters ...

Страница 76: ...can and Instrument Setup dialog is displayed 3 Click 4 In the Accessory Configuration section ensure that Fiber Optic Probe is selected in the Beam Path drop down list NOTE If you have a RamanFlex 400F with two fiber optic probes select Fiber Optic Probe 1 or Fiber Optic Probe 2 from the Beam Path drop down list as applicable 5 Ensure that the appropriate laser safety precautions are being observe...

Страница 77: ...ct Setup from the Instrument menu in Spectrum The Scan and Instrument Setup dialog is displayed 3 Click Spectral Preview Real time spectral acquisition will commence and spectra are displayed in the spectral preview tab 4 Set the Preview Scan time to 1 second and then press Enter This will automatically acquire another background You can enter a Preview Scan time in the range 0 1 10 seconds The sa...

Страница 78: ...al service or if there have been appreciable temperature variations in the local environment Backgrounds are always collected during a calibration procedure The background measures the bias and dark signal over the selected area of the CCD This is a necessary part of the calibration routines so it is always performed The spectrometer has no moving parts in its detection system therefore frequent c...

Страница 79: ... verification to ensure that the instrument has been brought back within specification If the wavelength calibration verification fails because the spectral intensity is low then this may also be due to the calibration sample holder not being correctly positioned optimally focused or because the laser key is not switched on Then repeat the wavelength calibration verification to ensure that the ins...

Страница 80: ...ment from the Instrument menu in Spectrum The Validation Calibration dialog is displayed 6 Select the Verification tab 7 Select Wavelength Calibration Verification and then click OK The wavelength calibration verification routine begins This will take a few minutes After completing the wavelength calibration verification routine an audit log is generated 8 Remove the polystyrene sample and store s...

Страница 81: ...egular basis the external neon calibration accessory is not included in the basic RamanFlex 400 package The external neon calibration accessory can be purchased separately NOTE If you have a fiber optic probe connected to the instrument ensure that it is covered 3 Select Validate and then Instrument from the Instrument menu in Spectrum The Validation Calibration dialog is displayed 4 Select the Ca...

Страница 82: ... screen that gives details on the outcomes of the laser frequency calibration In the unlikely event that the laser frequency calibration routine should fail please perform a wavelength calibration and repeat the laser frequency calibration The instrument in now laser frequency calibrated SOP 11 Intensity Calibration of the RamanFlex 400 The intensity calibration must be performed using the Nationa...

Страница 83: ...me of the intensity calibration In the unlikely event that the intensity calibration routine should fail please ensure the NIST sample is correctly positioned and that the laser key switch is set to on ARM If this is the case shut down the PC switch the instrument off restart the PC and switch the instrument back on and repeat the calibration 6 Remove the intensity correction standard and store sa...

Страница 84: ...maximum working pressure 1500 psi 5 m cabling L1320012 Raman probe max suitable for non contact and liquid immersion Maximum working temperature 500 C maximum working pressure 3000 psi 5 m cabling L1320013 Purge Jacket for Raman probe max required to enable max probe operation at temperatures between 200 C and 500 C L1320030 Triggered Fiber Optic Probe 5 m cable L1320031 Triggered Fiber Optic Prob...

Страница 85: ...Maintenance and Troubleshooting ...

Страница 86: ... not use abrasive or solvent based cleaning materials Always perform a patch test on an inconspicuous area before you clean the entire instrument Avoid spilling liquid into the instrument Clean all external spills immediately If anything that is spilled enters the main body of the spectrometer make the instrument inoperative and then contact a PerkinElmer Service Engineer WARNING Do not directly c...

Страница 87: ... unit 2 Disconnect the line power cord 3 Remove the 4 screws holding the top cover in position and then lift off the cover Figure 19 Removing the top cover of the spectrometer module 4 Unclip the filter covers remove the filters and wash in a mild detergent 5 Dry the filters thoroughly 6 Fit the air filters back into the spectrometer module and replace the covers 7 Place the top cover in position ...

Страница 88: ...ions regarding maintenance or servicing of the FOP The immersion sleeve if purchased is manufactured with 316 stainless steel or Hastelloy C and contains either a quartz or sapphire optical window that is sealed The quartz window can be removed for cleaning by unscrewing the end of the immersion tube The sapphire window cannot be removed as it is welded into place If removing the quartz window do ...

Страница 89: ...gnized Description This may mean that there is not enough power on the USB sockets on your PC Solution Ensure that your PC has USB 2 0 sockets If you are not sure use a USB 2 0 power hub Problem You are prompted for the CCD camera drivers more than once Description This may happen if you disconnect the instrument control USB cable and reattach it to a different USB port or if you switch the instru...

Страница 90: ... with the installation in the same way as for IDUS 2 Select Install from a list or specific location Advanced and then click Next The Please choose your search and installation options page is displayed 3 Select Include this location in the search click Browse and then navigate to C Program Files PerkinElmer ServiceIR Raman These options may be displayed automatically as you work through the wizar...

Страница 91: ...is breached Description This means that for some reason the interlock system is detecting an open circuit Solution 1 Ensure all interlocks including any remotely interlocked doors are closed properly Solution 2 Ensure your external interlock is wired up correctly and that any interlocked room entrances or sampling enclosures are closed ...

Страница 92: ...and open the Spectrum software Problem During system initialization the laser does not stabilize Description The laser initialization form displayed during system initialization runs for over 60 seconds During this time the laser should have stabilized Solution Ensure that the laser key on the front panel of the spectrometer is set to the ARM position Problem Detector cannot achieve operational te...

Страница 93: ...ntamination The customer s responsible body is required to follow the Equipment Decontamination Procedure and complete the Certificate of Decontamination These documents are available on the PerkinElmer public website http las perkinelmer com OneSource decontamination htm Alternatively if you do not have access to the internet contact Customer Care Customer Care USA 1 800 762 4000 inside the USA 8...

Страница 94: ...is the most effective means of protecting the customer s environment The requirements for waste collection reuse recycling and recovery programs are set by the regulatory authority in your location Contact your local responsible person such as your laboratory manager or authorized representative for information regarding applicable disposal regulations See the PerkinElmer web address below for inf...

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