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CERO_Operators Guide_Version 2018-12_01 

 

 

16

 

 

3.1.3 Culture Protocol 

After  a  successful  inoculation  cell  expansion  is  easily  accomplished.  The  Standard  Culture 

Protocols (figure 3) consist of the same parameters as the Inoculation Protocol. Since Culture 
Protocols  usually  do  not  need  conditions  as  gentle  as  Inoculation  Protocols,  the  default 
parameters have different settings e.g. the Agitation Pause is switched off (

none

). Nevertheless 

more gentle conditions can be set with the same functionality as in Inoculation Protocol mode. 

 

 

 

 

 

 

 

 

 

 

 

 

Figure  3: S tanda rd  Cultu re Protocol  

 

3.1.4 Harvesting Protocol 

A standard Harvesting Protocol (figure 4) consists of the following parameters:  

- 

Rotation Period

: period of rotation into one direction  

- 

Rotation Pause

: pause between 2 rotation periods 

- 

Rotation Speed

 

For Harvesting no agitation is needed so the parameters Agitation Period and Agitation Pause 

were excluded from this protocol. 

 

 

 

 

 

 

 

 

 

 

 

Figure  4: S tanda rd  Harves ting Protocol  

Содержание 2800000

Страница 1: ...CELL CULTURE SIMPLIFIED...

Страница 2: ...CERO_Operators Guide_Version 2018 12_01 2...

Страница 3: ...GmbH Co KG OLS All Rights Reserved Contents of this Operators Guide are subject to change without prior notice Technical modifications reserved Greatest possible care was used on the correctness of th...

Страница 4: ...ell cultures In addition the CERO eliminates traditional peripheral cell culture instruments such as incubators and centrifuges and minimizes manual handling With proper care and maintenance your new...

Страница 5: ...1 2 Operating the CERO 12 2 2 Hazards of CO2 13 2 3 Electrical Safety Precautions 13 2 4 Biohazard Precautions 13 3 PRODUCT DESCRIPTION 14 3 1 Culturing Cells in the CERO 14 3 1 1 Parameters Protocol...

Страница 6: ...Set CO2 Offset Compensation 30 5 6 Create a New Experiment 31 5 7 Insert a CEROtube in the CERO 32 5 8 Start an Existing Experiment 33 5 9 Follow up a Running Experiments 34 5 10 View the Output of a...

Страница 7: ...olor Mode 47 6 4 4 Adjust the Settings for the Color Monitoring 48 6 4 5 Adjust the Settings for the pH Monitoring 48 6 4 6 Start an Existing Experiment with enabled pH Reader 49 6 4 7 Follow up the p...

Страница 8: ...igh 60 8 3 Tube Rotation Interrupted 61 8 4 Convection Channel Interrupted 61 8 5 Malfunctioning User Interface 61 8 6 Sliding Top Cover Does Not Open 61 8 7 Getting Technical Support 62 8 8 System In...

Страница 9: ...address below OMNI Life Science GmbH Co KG Karl Ferdinand Braun Str 2 28359 Bremen Telefone 0421 27 61 69 0 e mail info ols bio de Online Support CERO online is available on www ols bio de direct onl...

Страница 10: ...2014 35 EC relating to Low Voltage Equipment The following standards were applied EN 61010 1 2001 and EN 61010 2 081 2002 Safety EN 61326 2013 EMC Emision Class B and EN61326 1 2013 EMC Immunity IV Wa...

Страница 11: ...d be capable of operating the CERO Warnings are included in this Operators Guide to emphasize important and critical instructions They are printed in bold italics and begin with the word ATTENTION acc...

Страница 12: ...le dangers involved Only certified technicians are authorized to perform mechanical maintenance on the CERO Necessary precautions should be taken when transporting the instrument For repair or shipmen...

Страница 13: ...stated on the power rating in the Technical Specifications see Chapter 9 Avoid damaging the power cord Do not bend it excessively step on it place heavy objects on it A damaged cord can easily become...

Страница 14: ...1 Culturing Cells in the CERO using the example of a microcarier culture The initial inoculation phase supports the aggregation of the cells or the loading of cells on the microcarrier substrate The c...

Страница 15: ...irection Rotation Pause pause between 2 rotation periods Rotation Speed During inoculation it is critical to ensure adequate aggregation of cells or adhesion of the cells to the Microcarriers Most cel...

Страница 16: ...e g the Agitation Pause is switched off none Nevertheless more gentle conditions can be set with the same functionality as in Inoculation Protocol mode Figure 3 Standard Culture Protocol 3 1 4 Harvest...

Страница 17: ...g sections 3 3 Culture Chamber 3 3 1 Sealed Culture Chamber The sealed compact Culture Chamber figure 6 maintains consistent CO2 and temperature levels during cell expansion The removable Convection C...

Страница 18: ...emperature Control Integrated with compact controllers the CERO ensures proper control of environmental temperature and CO2 levels figure 7 The Chamber Access Port on the Top Cover enables monitoring...

Страница 19: ...ith Click Wheel figure 9 allows for quick navigation and provides users with intuitive and easy to program cell culture workflows In addition culture reports including temperature and CO2 monitoring c...

Страница 20: ...ersion 2018 12_01 20 3 5 CERO Software The CERO Software is structured in 4 Menus all accessible from the Main Menu see figure 10 the System Menu the Experiments Menu the Outputs Menu the Run Menu Fig...

Страница 21: ...System Menu Das System Men bietet Zugriff auf alle Systemfunktionen und einstellungen System Settings see Chapter 5 13 Maintenance see Chapter 7 Troubleshooting see Chapter 8 System Information see C...

Страница 22: ...the top of the Mains Power Socket To gain access to the Fuse Holder you must first remove the Mains Power Cable figure 11 Only use orifinal OLS spare parts or fuses with correct specification see cha...

Страница 23: ...on and USB Port 3 6 5 Communications Ports The USB Port is used to export experiment outputs or upload software updates It is located on the front of the CERO in the lower right hand corner figure 12...

Страница 24: ...s In ordert o use the CERO you need Requirement Description Mains Electrical Supply 85 240 V AC 85 240 VAC 200 VA CO2 Gas Cylinder Cylinder with medical grade 100 CO2 vapour withdrawal Dual Stage Pres...

Страница 25: ...rument Unpack the instrument if necessary with assistance of a colleague Inspect instrument and accessories for shipping damage or missing parts Use packing list to verify contents If any items are mi...

Страница 26: ...ing with 3 16 4 8 mm inner diameter figure 14 Make sure that the connection is tight and secure Figure 14 CO2 supply connection IMPORTANT NOTE The proper and secure connection of the CO2 supply to the...

Страница 27: ...ure Control Window figure 16 from the Main Menu 2 Pressing the Temperature Round Button 3 The background of the Temperature Button changes colour and the range of adjustment is displayed 4 Rotate the...

Страница 28: ...en the convection channel is not installed or damaged 1 Enter the CO2 Control Window figure 17 from the Main menu 2 Press the CO2 Round Button 3 The background of the CO2 Button changes colour and the...

Страница 29: ...he temperature regulation at 37 C as described in Chapter 5 2 3 After 1 hour open the Chamber Access Port on the Sliding Top Cover by removing the Coin Screw 4 Measure the temperature of the liquid in...

Страница 30: ...llow the CO2 levels to stabilize for at least 20 minutes 3 Insert a CO2 incubator analyser probe into the coin screw opening in the sliding cover of the CERO and measure the CO2 concentration NOTE We...

Страница 31: ...iment and enter the experiment name 3 Open the Inoculation protocol by selecting the Inoculation Button figure 20 4 Select the Protocol Type with the Type Button Figure 20 Inoculation Protocol 5 Set t...

Страница 32: ...For security reasons the CO2 supply stops while the sliding cover is open It starts again automatically as soon as the door closes again 2 Insert the CEROtube in the holder of the desired position 3...

Страница 33: ...l be run with the same experiment you can select the desired number of tubes with the select multiple button figure 10 3 The Run Menu figure 21 will appear Figure 21 Run Menu of Tube 1 4 Press the Sta...

Страница 34: ...can be viewed on the graphical panel on the left hand side of the screen 4 Click on the graphical panel to enlarge it 5 The trace provides a record of the experiment messages with the chosen message...

Страница 35: ...t side of the CERO 2 If necessary format your memory stick in the Maintenance Menu chapter 5 16 3 Go to the Outputs Menu figure 24 4 Select the output to export in the list 5 Press the Export Button N...

Страница 36: ...nctional Verify the integrity of the CO2 supply 5 13 2 Tolerance of the Out of Range Notification CO2 and Temperature The CERO notifies out of range environmental conditions temperature and CO2 level...

Страница 37: ...es the size of the Outputs file To modify the level of message proceed as follows 1 In the System Menu go to Settings 2 Set the sesired message level normal for standard use detailed for higher comlia...

Страница 38: ...nfigurations Scwitch Position IP Configuration 0 Predefined IP settings The IP address assigned is 192 168 1 1 1 Configurable IP settings Please ask your network administrator for the appropriate sett...

Страница 39: ...elete all Outputs 5 16 Format USB Stick 1 Go to System and select Maintenance 2 Insert Memory Stick 3 Press the Format USB Stick Button 5 17 Transfer Experiments between two CEROs The first step of th...

Страница 40: ...vice 6 1 2 Precautions when installing the Reader Do not attempt to install the Reader while the CERO is in operation Switch off the CERO and disconnect the power cable before installing the Reader Wh...

Страница 41: ...qualitative color mode and a semi quantitative pH mode In the color mode the absorbance of the medium at 560 nm is directly reported It increases when pH increases and the medium turns red figure 27 F...

Страница 42: ...EROtube A minimum medium volume of 35 mL is required for pH monitoring Figure 28 pH Measurement Step by Step 6 2 4 Example Outputs 6 2 4 1 In the Color Mode The pH of the cell culture medium decreases...

Страница 43: ...e notification level has been set at 6 8 Figure 30 Example output in the pH mode Error Estimation The pH mode allows estimating the pH of the cell culture in the CEROtube by a non invasive optical abs...

Страница 44: ...e CERO and disconnect the power cable Remove the Front Cover see chapter 7 3 Loosen the thumb screw A and remove the Reader Blanks Figure 31 Figure 31 Removal Reader Blanks ATTENTION Avoid contact wit...

Страница 45: ...arrows on figure 33 Please note that both Readers are identical and may need to be turned 180 for positioning in front of the lower right connector or higher left connector Figure 33 Positioning of t...

Страница 46: ...4 Check the Box Enable Reader figure 35 Figure 35 Reader General Settings 6 4 2 Set General Reader Settings 6 4 2 1 Set the Time Interval between two Measurements 1 In the System Menu go to Settings 2...

Страница 47: ...uantitative pH Measurement by pressing the Minimal pH Button 4 Adjust the notification level of the qualitative color measurement by pressing the Minimal Color Button 5 Rotate the click wheel to set t...

Страница 48: ...nt starts 6 4 5 Adjust the Settings for the pH Monitoring 1 In the System Menu go to Settings 2 Enter the Reader Window and select pH Settings figure 35 5 Verify that the pH Mode is enabled and enable...

Страница 49: ...he Run Menu figure 38 will appear Figure 38 Run Menu of Tube 1 3 Press the Start Button 4 Select the desired experiment in the list and press Ok 5 In the Start Window figure 39 you may enter a comment...

Страница 50: ...Measurement 8 Place the culture CEROtube in the desired tube position 9 Press Start to begin the Experiment 6 4 6 2 Mode pH 1 In the Main Menu select the tube position where the experiment will be ru...

Страница 51: ...ating the measured E0 value figure 42 Please repeat the blank measure if the standard deviation is above 20 NOTE The Blank Measure enables the zero calibration of the Reader Figure 42 Blank Measuremen...

Страница 52: ...e graphical panel to enlarge it 6 4 8 Single Point Measurement during a Running Experiment It is possible to trigger a single point measurement of pH respectively color while an experiment is running...

Страница 53: ...rforming the weekly maintenance 2 Perform the decontamination procedure described in Chapter 7 2 3 Export the output files run during the week 4 Check available memory space see Chapter 7 8 NOTE The C...

Страница 54: ...eplace the Convection Channel see Paragraph 7 4 7 3 Remove and Re Install the Front Cover 1 Open the Sliding Top Cover 2 Switch off the CERO 3 Loosen the thumb screw securing the front cover see figur...

Страница 55: ...the top and bottom to free it from the chamber figure 46 Figure 46 Remove the Convection Channel To re install the Convection Channel back in place proceed as follows 1 Position the Convection Channel...

Страница 56: ...y maintenance or service on this unit disconnect the Main Power Cable from the power supply Always wear suitable protective clothing safety glasses and protective gloves during maintenance 7 7 1 Weekl...

Страница 57: ...Information figure 49 2 Check the amount of memory space available for saving new Outputs under Storage Data 3 We recommend freeing memory space and export or delete Outputs if Storage Data is 80 full...

Страница 58: ...e sensor defect Call customer support 8 1 2 Temperature Too Low Possible explanation Recommended action 1 Offset value too low Readjust temperature offset chapter 5 4 2 CERO covers open Close the CERO...

Страница 59: ...8 2 3 5 Unidentified Switch off the CO2 supply and call customer support 8 2 2 CO2 Level Too High Possible explanation Recommended action 1 Offset value too high Readjust CO2 offset chapter 5 5 2 Conv...

Страница 60: ...ommended action 1 Cover being opened too often Close the door and allow CO2 levels to settle 2 Convection channel defect Replace convection channel chapter 7 4 3 Atmospheric pressure fluctuations Read...

Страница 61: ...1 User interface not responding Restart CERO 2 Software incomplete or damaged Upload latest software version chapter 5 14 3 Mechanical damage to user interface Call customer support 8 6 Sliding Top C...

Страница 62: ...tomer Support The following information may be requested to support you in solving the identified problem 1 Device part number and serial number as indicated on unit label at the back of the instrumen...

Страница 63: ...Abbildung 50 System Information Window 8 9 Export System Data System Data may be requested by your support team to help characterise and solve a problem you encountered To Export the all System Data p...

Страница 64: ...mber This number assures proper tracking of your instrument Instruments that are returned without an RGA number will be sent back to the customer without being repaired ATTENTION OLS reserves the righ...

Страница 65: ...ed sensor Range 0 18 Control 0 1 Operation Maximum power consumption 200 VA Voltage 100 V 240 V AC 10 Frequency 50 60 Hz Delayed action fuse T4 A 250 V 5 x 20 mm Temperature range 15 C 28 C Relative h...

Страница 66: ...e dimensions L x H x D 158 x 45 x 58 mm 6 2 x 1 8 x 2 3 in Module weight 630 g 1 4 lb Operation Temperature range 15 C 28 C Relative humidity range 15 85 non condensing Location Only mounted to the CE...

Страница 67: ...Instrument Part number Description 2800000 CERO benchtop 10 2 Consumables Artikelnummer Beschreibung 2800005 CEROtube pack of 48 2800006 MICROLAB Disinfectant spray kit 2800003 Convection channel 2800...

Страница 68: ...rent experiments one for each tube can be run independently Harvesting Last and optional step of cell culture It refers to the collection of cells for downstream applications Inoculation Initial step...

Страница 69: ...CERO_Operators Guide_Version 2018 12_01 69...

Страница 70: ...For life science research only OMNI Life Science GmbH Co KG Karl Ferdinand Braun Stra e 2 28359 Bremen T 49 421 276 16 9 0 F 49 421 276 16 9 19 info ols bio de www ols bio de...

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