GeminiBio ORFLO Moxi V, Руководство пользователя

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Moxi V
™
User Guide
Page 30
The up/down arrows on the histogram can be used to resize the histogram y-axis (scaling). The
“Print BMP” button will save a Bitmap (image, .bmp) file to the Moxi V data disk (in the main
“Test-Files” folder for single runs or the batch folder for batch runs). That image will be an exact
representation of the “Results Summary” screen.
Touching the “Save” button for a Single Run Cell QC test, will save the data (.FCS format) and
will return the system to the Home Screen. For Batch Runs, the system will proceed to walk the
user through loading more samples as described below in the “Cell QC – Test Output –
Continuing Batch Testing” section.
Analyzing/Gating the Data
For tests other than the PBMC, CAR-T, and Cell QC tests, the test results are presented to the
user immediately following a test in the form of a scatter (dot) plot (below/left) for fluorescent
tests or as a size-only histogram (below/right, “Size Histogram” test only).
Note: Details on the
PBMC, CAR-T, and Cell QC tests test outputs can be accessed as described in their respective
sections of the User Guide.
Depending on the global setting of the “Auto Noise-find” value (see
“Settings” section for details on changing values), the system may initially auto-gate the data.
Whether or not the data is auto-gated, the user will still have the option to adjust/fine-tune the
gates manually.
Fluorescence data is presented using Logicle scaling. Logicle scaling is similar to biexponential
scaling in flow cytometry. It is implemented to allow for the presentation of negative data (that
might result from compensation or baseline averaging) and to better represent the variance of
the data by compressing the data region around zero.