Moxi V
™
User Guide
Page 24
Reading Raw Data Output (Conc., Size, MFI, and %)
The Moxi V™ provides particle concentration and precise sizing information for each gated
region, total counts information, fluorescent percentages, and median fluorescent intensities (all
where applicable based on the channels selected) for each test. Two example test view
screens are shown above (left to right: Fluorescence vs. size scatter (dot) plot, Single Channel
Histogram Display).
Fluorescence vs. size scatter (dot) plot
Image above/left:
For this plot, statistics are only generated for the size-gated (between the
blue markers), noise excluded (points that aren’t grayed out following a test) region. The total
concentration (provided as particles/ml) is listed above the scatter plot between the blue gate
region (1.924 x 10
5
cells/ml in the example above/left). The two black boxes below the scatter
plot provide the subset concentration (cells/ml), mean particle diameter (
Note: This is an
“effective” diameter that is based on a precise volumetric measurement (Coulter Principle)
combined with a spherical assumption.
), particle volume (Coulter-Principle measured), and
median fluorescence intensity (MFI) values for the regions below (“Lower population”) and
above (“Upper Population”) the red fluorescent gate (but within the size-gated region). The
percentage of events that fall above/below the fluorescence (red) gate is displayed in the yellow
box above the scatter plot (e.g. 39.4% in this example).
Single Channel Histogram Plot
Image above/right:
An example of the on-unit histogram display for the PIN diode (561nm/LP
fluorescence) Only the points within the size-gated region for the fluorescence channel vs. size
plot (e.g. image above/left) are included in generating a histogram. This is deliberately done to
avoid having noise contributions in the histogram. The red and green shading are determined
by the positioning of the fluorescence (red) gate marker on that same fluorescence vs. size plot
(image above/left) with the red representing the fluor points (above the red line) and
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