GeminiBio ORFLO Moxi V, Руководство пользователя

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Moxi V
™
User Guide
Page 14
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It is best to place the pipette facing straight into the system (not angled left or right)
and a ~45 degree angle (up/down, see image below)
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Dispense the fluid in one, smooth motion as you would normally during pipetting.
The system does apply a slight vacuum to pull the fluid in, but it is not recommended
to try to “time the load” to the draw of the fluid. It is normal/expected to have a bead
of fluid form over the entry well. That fluid will get pulled in eventually.
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The system provides the user a 60 second window to complete each step (opening
the door, loading the sample, waiting for the fluid to reach the start electrode). If the
time limit for any of the steps is exceeded, the test will automatically cancel and the
user will be returned to the
Home screen.
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The instrument will begin the test. While the system waits for sample/fluid detection an
“Analyzing…” message is indicated in top left of the screen a countdown until the test is
auto-cancelled (auto cancel occurs after 60 seconds if no fluid is detected). Once the
fluid is detected, the system message changes to “Counting…” and the results are
dynamically populated on the scatter/dot plot. Results for most samples will be complete
in approximately 10 seconds but may be longer for higher concentration, sticky, or
aggregated samples.
Please refer to the “Test Results – Analyzing/Gating Data”
section for details on gating and interpreting the data output for all tests.
“Cell QC” Test
The “Cell QC” app is designed to provide simplified analysis of cell viability samples either one
test at a time or in batch mode (rapid acquisition of multiple samples of the same cell type, using
consistent gating). The initial display output of the Cell QC app is a simplified, list of the sample
statistics, based on the system’s auto-gating of the sample. The batch mode, in-addition to
providing for rapid acquisition of data, provides the same simplified output for each test, along
with a final summary .csv (e.g. Excel) output file with the summary data for all tests.
Touching the “Cell QC” icon on the Home screen brings up the Cell QC “Enter Sample Info”
screen. The first selection option is the “File Option”. There are three file options: 1.) “Single
Run” (run a single test). 2.). “Start a Batch” (run a series of samples of the same type in rapid
succession) 3.) “Add to Existing Batch” (continue running a series of samples in batch mode,