GE Ettan IPGphor 3, Руководство пользователя прибора

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Ettan IPGphor 3 Isoelectric Focusing System function and description
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Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
• for larger sample volumes the Ettan IPGphor Cup Loading Manifold
facilitates paper bridge loading and in gel rehydration loading
The Ettan IPGphor 3 Control Software has an easy to use interface to the Ettan
IPGphor 3 instrument and provides enhanced protocol generation, presentation,
and storage.
Recommended standard protocols can be retrieved and used by the user simply
providing the instrument configuration, IPG strip length and pH gradient. Using
the advanced mode setting, earlier made custom protocols or entire new
protocols are made. All protocols are edited and saved for later use. The selected
protocol is loaded to Ettan IPGphor 3 Isoelectric Focusing Instrument.
During the run the control software monitors and records the running
parameters. All data is displayed as graphs and log file tables. When the run is
complete, graph and log files are printed or/and saved. If desired, the log files are
exported to programs such as Microsoft Excel.
The Ettan IPGphor 3 Control Software controls up to four Ettan IPGphor 3 units at
one time, each running a different set of run parameters.
IPG strip manifolds are available in two models:
• One produced from a ceramic material providing greater temperature
uniformity
• One produced from a light polymeric material for less critical
experimentation
Both are designed for analytical analysis using cup application and preparative
runs applying sample to the gel during the rehydration step or by paper bridge
loading. Using the Manifold Immobiline DryStrips are rehydrated in the
Rehydration Tray.
Fixed length strip holders are available. Once the IPG strip is placed in the fixed-
length strip holder, no further handling is required. The holder is placed on the
Ettan IPGphor 3 electrode platform for rehydration and can be immediately
followed by isoelectric focusing. The sample is introduced during the rehydration
step. The Ettan IPGphor 3 accommodates up to 12 strip holders for any one
protocol.
IPG strips are available in five lengths 7, 11, 13, 18, and 24 cm, and a number of
pH ranges, several linear and non-linear (listed in section 6.2 Ordering
information).