Operating instructions
3
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
25
3.3
Using fixed-length Ettan IPGphor 3 strip holders
Ettan IPGphor 3 fixed-length strip holders allow IPG strips to be rehydrated and
samples loaded in one step before proceeding automatically to perform the
separation. The IPG strips are 3 mm wide and 0.5 mm thick after rehydration.
Fig 3-2.
Strip holder.
3.3.1
Preparing the strip holder(s).
1
Select the strip holder(s) corresponding to the IPG strip length chosen for the
experiment (7, 11, 13, 18, or 24 cm).
2
Wash each holder with the strip holder cleaning solution supplied to remove
residual protein.
3
Rinse thoroughly with double distilled water.
4
Use a cotton swab or a lint-free tissue to dry the holder or allow it to air-dry.
The holder must be completely dry before use.
WARNING!
Check that the high voltage is turned off before opening the safety
lid. The HV ON indicator on the front panel should not light.
Cover
Pressure
block
Sample
application wells
Base
IMPORTANT!
Handle the ceramic holders with care, as they are brittle and
fragile.
Содержание Ettan IPGphor 3
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