GE Ettan IPGphor 3, Руководство пользователя прибора

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Ettan IPGphor 3 Isoelectric Focusing System function and description
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Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
Platform The platform accommodates one manifold for the first dimension isoelectric
focusing (IEF) run.
Up to 12 fixed-length strip holders can be placed on the platform for rehydration
and IEF.
The embedded electrode areas provide the electrical connection between strip
holder or manifold electrodes and the integral power supply.
Platform temperature is controlled by Peltier thermoelectric modules.
Electrode areas Both electrode areas are gold-plated copper. Placing a manifold or a strip holder
so that it bridges both electrode areas completes the electrical circuit (once the
safety lid is in position).
Safety lid The safety lid must be properly closed before power is applied.
Light protective cover When analyzing proteins labeled with light sensitive stains like CyDye®, a light
protective cover (Fig 1-2) is used. The cover is applied by magnetic force but is
easy to partially remove for checking the isoelectric focusing procedure,
especially the migration of bromo-phenol-blue at start.
Lid adapter for strip
holders When using the Ettan IPGphor 3 standard strip holders a lid adapter (Fig 1-6) must
be used to apply the correct amount of pressure to the lids of the Ettan IPGphor 3
strip holders.
Ettan IPGphor 3
Control Software Ettan IPGphor 3 Control Software allows the user to control the Ettan IPGphor 3
Isoelectric Focusing Instrument. The software provides an enhanced functionality
in the areas of data presentation, data storage, and protocol handling. The PC is
connected to the Ettan IPGphor 3 over a serial cable or USB converter.
Ettan IPGphor 3 Control Software controls up to four Ettan IPGphor 3 units at one
time, each running a different set of run parameters.
Manifolds
Ettan IPGphor Cup
Loading Manifold
Ceramic The manifold (Fig 1-4) is made of aluminum oxide ceramic for efficient heat
transfer and temperature control during IEF. The manifold accomodates up to 12
IPG strips from 7 cm to 24 cm long. Immobiline DryStrip is placed with the gel side
facing up.
Samples are applied in a cup. Cup loading has been found to improve protein
focusing patterns, particularly on basic IPG strips (pH 6-9 and 6-11). Larger
sample volumes can be applied by gel rehydration loading or anodic paper
bridge loading.
Protrusions along the channel inside the manifold align the rehydrated IPG strip,
keeping it straight and centered when placed inside the manifold. The manifold
has a coating to minimize protein adsorption. The unit may also be used for IPG
strip equilibration prior to second dimension electrophoresis.
The manifold is fragile and should be handled with care.