GE Ettan IPGphor 3, Руководство пользователя прибора

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Troubleshooting
5
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
55
5 Troubleshooting
Symptom Possible solution
Problems indicated by LCD messages
Lid open step 1, close to continue The safety lid is not properly closed. When the safety lid is open, the
system has an automatic voltage cutoff safety feature. In order for the
protocol to proceed, the safety lid must be closed.
Locked screen in edit mode Turn off the mains power switch to reset the instrument.
Blank display If no electrical components are functioning (e.g. HV lamp does not light
and the cooling fans are motionless), check the fuses in the mains
power module.
Diagnostic program indicates
component failure Note the component that failed and press the START key to continue
through the diagnostic program. Call your local GE Healthcare service
for further information on how to remedy the failure.
Arc An electric arc has been detected. The instrument will reduce the
voltage setting 500 volts automatically. The run continues, and there is
no need for further action.
Power delivery
Current too low or zero At least two of three pressure pads under the safety lid should press
gently against the strip holders to ensure electrical continuity between
the strip holder electrodes and the electrode areas on the platform.
The gel must be evenly and completely rehydrated to conduct current.
Make sure the proper amount of rehydration solution is applied to the
IPG strip holder and allow a minimum of 10 hours for rehydration.
Check that both connecting points of the strip holder or the connecting
pins of the manifold electrodes are in contact with the gold surface.
Voltage limit not reached The ionic strength of the rehydration solution is too high. The instrument
has reached the current limit; reduce the IPG buffer concentration; use a
mixed-bed ion-exchange resin to remove ionic breakdown products of
urea of other additives.
Desalt the sample or prepare the sample so that the salt concentration
is less than 10 mM.