GE Ettan IPGphor 3, Руководство пользователя прибора

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Operating instructions
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Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
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3.2 Using the Ettan IPGphor Cup Loading Manifold
For more detailed information, refer to Ettan IPGphor Cup Loading Manifold User
Manual.
3.2.1 Rehydrating Immobiline DryStrip in the Rehydration Tray
Rehydrate the IPG strips with the gel side down in the appropriate volume of
rehydration solution, with or without sample, using the Immobiline DryStrip
Reswelling Tray.
Rehydration in the Cup Loading Manifold is not recommended, the channel is too
wide to ensure proper rehydration.
Also, follow the instructions included with the Immobiline DryStrips. Cover the IPG
strips with Immobiline DryStrip Cover Fluid, and allow the strips to rehydrate
overnight (10-20 hours).
3.2.2 Positioning the manifold on the Ettan IPGphor 3
1 Clean and dry the Ettan IPGphor 3 platform before placing the manifold on
the unit.
2 Position the manifold on the Ettan IPGphor 3 platform. The small T-shaped
protrusion fits into a cutout section of the Ettan IPGphor 3 platform near the
lid hinge.
3 Ensure that the manifold is level by placing the round spirit level on the
center of the manifold tray after it is placed on the Ettan IPGphor 3 unit.
4 Measure out 108 ml of Immobiline DryStrip Cover Fluid (even if fewer than 12
strips will be loaded into the manifold). Add the cover fluid evenly in the 12
manifold channels.
3.2.3 Positioning the IPG strips in the manifold
1 Transfer the strips to the Ettan IPGphor Cup Loading Manifold.
2 Position the strips under the cover fluid face up in the tray with the anodic
(+, pointed) end of the IPG strip pointing at the anode of the Ettan IPGphor 3.
3 Center the strip down the length of the manifold channel. Protrusions along
the sides guide the strip approximately straight, although some manual
adjustment of the strip may be necessary. See also the
Ettan IPGphor Cup
Loading Manifold User Manual .
WARNING! Check that the high voltage is turned off before opening the safety
lid. The HV ON indicator on the front panel should not light.