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8.5 After run procedures
Introduction
This section describes how to clean the instrument and columns after a chromatographic
run, and how to prepare the system for storage.
The instrument and the columns should be cleaned between the runs. This will prevent,
for example, sample contamination, protein precipitation and column clogging. If the
instrument is not going to be used for a couple of days or longer, the instrument, columns
and the pH flow cell should be filled with storage solution. For further information about
cleaning and maintenance procedures, see User Manual.
TIP:
To clean and fill the instrument and columns with storage solution,
use System CIP and Column CIP either as separate, predefined
methods or as phases included in a chromatographic method.
WARNING
Hazardous chemicals during maintenance. When using hazardous
chemicals for system or column cleaning, wash the system or
columns with a neutral solution in the last phase or step.
System cleaning
After a method run is completed, perform the following:
•
Rinse the instrument with one or several cleaning solution(s) (e.g., NaOH, buffer
solution or distilled water) using System CIP.
•
Empty the Fraction collector.
•
Clean all spills on the instrument and on the bench using a moist tissue.
• Empty the waste vessel.
• Check that the pH electrode is in appropriate buffer.
System storage
If the instrument is not going to be used for a couple of days or longer, also perform the
following:
•
Fill the system and inlets with storage solution (e.g., 20% ethanol) using System CIP.
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB 111
8 Run a method
8.5 After run procedures