GE AKTA avant, Начало работы, Страница: 64 / 134

Choose a maintenance method
A number of predefined methods for preparation and cleaning are available. Use these
maintenance methods to prepare and clean the system and columns, and to fill the
system and columns with storage solution. The table below gives suggestions for what
methods and solutions to choose for the different purposes.
Solution Phase/Method If you wish to...
suitable buffers System Preparation Prepare tubing and instrument modules
before a run
suitable buffers Column Preparation Prepare column before a run
0.5-1 M NaOH System CIP Clean tubing and modules
0.5-1 M NaOH Column CIP Clean column
20% Ethanol System CIP Leave system in storage solution
20% Ethanol Column CIP Leave column in storage solution
TIP:
CIP (Cleaning-In-Place) and preparation of columns and system may
be run either as separate predefined methods or as phases included
in chromatographic methods.
TIP:
The System CIP and System Preparation phases are designed to use
one cleaning solution each. The System Preparation method includes
two System Preparation phases and the System CIP method includes
three System CIP phases. To use additional solutions sequentially, add
phases to the method.
Note: Usually 0.5-1 M NaOH is used for cleaning of the system and column.
However, before choosing cleaning agent always consider the media
and buffers used, and also the chemical resistance of the column.
64 Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
6 Create a method
6.1 Guide to method creation
6.1.1 Create and edit methods