flexcell Flex Jr. Скачать руководство пользователя страница 20

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

13 

regimen at its current position so that it can be 
resumed from where it paused. 
 

3.13

 

 D

ETERMINE THE 

P

ROGRESS OF 

T

HE 

R

EGIMEN

 

 
The progress of the regimen for the currently 
selected  FlexLink

®

  will  be  monitored 

continuously.  The  displays  for 

Run  Time

Step

  number, 

Cycle

  number, 

Jump

  to 

number, and 

Min

 and 

Max

 % elongations will 

be updated in  the upper right  hand corner of 
the  main  display  window.  The  actual 
waveform data returned by the controller will 
also be continuously updated on the graph. A 
timeline progress bar below the plot illustrates 
the  percent  of  the  regimen  that  has  been 
completed. 
 

3.14 R

ESETTING THE 

F

LEX

L

INK

®

 

 

 
Resetting  a  simulated  or  running  regimen 
causes the current activity to stop at the next 
cycle  of  the  current  regimen  step,  and 
disassociate the regimen from  the  FlexLink

®

 

controller. To reset, click on 

Reset 

in the main 

display  window.  Click  on 

Yes 

when  the 

program  asks  for  confirmation.  The  system 
will now be in state similar to that which exists 
when the Flex Jr.

 Tension FlexLink

®

 is first 

turned ON. 
 

3.15 D

ATA 

A

NALYSIS

 

 
The  Flex  Jr.

  software  has  the  capability  of 

storing data (a set of waveform points) from a 
regimen  run  to  a  file  on  the  hard  drive.  The 
filename consists of a “E” or “P” code letter 
(

E

  for  elongation,  or  P  for  pressure),  the 

FlexLink

®

  number,  a  “V”  (for  vacuum),  the 

assigned  baseplate  platform,  the  user,  the 
regimen  name  and  a  sequence  number.  If  a 
tension  regimen  is  run  three  times  on 
FlexLink#1, then there will be three data files 
on the hard drive with numbers ‘000’, ‘001’ 
and 

‘002’, 

respectively 

(i.e., 

E_1_V_BaseplatePlatform_User_Regimen
Name_000.dat, 
E_1_V_BaseplatePlatform_User_Regimen
Name_001.dat,  etc.).  The  file  size  depends 
on the regimen duration. The current settings 
take pressure data every 2 milliseconds (i.e., 
500  points/sec  or  1,800,000  points/hour). 
Therefore,  a  one  hour  regimen  will  have 
approximately  a  4.0  Mb  data  file.  To  turn 
this feature OFF, please contact Flexcell

®

 
To see the stored data, in the 

Data Analysis

 

menu,  select 

Data  Viewer

.  Open

 

the 

appropriate  .dat  file  from  the  C:\FlexSyst 
directory.  A  new  window  will  appear  (Fig. 
12). The data presented  defaults  to  the  raw 
data.  Moving  average  can  be  selected  to 
display  the  raw  data  with  data  averaged 
every 10 seconds.  
 
The 

y-Axis control 

box

 

allows for automatic 

or manual control of the scale on the y-axis. 
If 

Auto 

is  selected,  the  y-axis  scale  can  be 

changed by clicking on one of the two small 
double-arrow  buttons  at  either  end  of  the 
axis. The button with arrows pointing away 
from  each  other  increases  the  axis  scale, 
while  the  button  with  arrows  pointing 
toward  each  other  decreases  the  scale.  The 
minimum value is -2 kPa and the maximum 
is -100 kPa. If 

Manual

 is selected, the user 

can enter the upper and lower limits of the y-
axis scale in the white boxes to the left side 
of the axis. The small double-arrows will not 
function if 

Manual

 is selected. 

 

 

Figure 12.

  Flex Jr.

 data display 

 

Содержание Flex Jr.

Страница 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Страница 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Страница 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Страница 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Страница 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Страница 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Страница 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Страница 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Страница 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Страница 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Страница 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Страница 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Страница 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Страница 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Страница 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Страница 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Страница 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Страница 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Страница 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Страница 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Страница 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Страница 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Страница 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Страница 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Страница 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Страница 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Страница 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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