background image

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

coordinates

  for  the  waveform.  These  are 

displayed  when  the  left  mouse  button  is 
clicked  and  held  down  in  the  graph  area. 
When this is done, a pair of crosshairs appears 
and the coordinates at  the intersection of the 
crosshairs are shown in  the X and Y spaces. 
The crosshairs and values will track the mouse 
as long as the left mouse button is held down. 
 
The next block to the right is a section used to 
display the current 

User

Regimen

Platform

and 

Run time

. Run time has two blocks. The 

one  on  the  left  displays  the  elapsed  regimen 
time,  and  the  one  on  the  right  displays  the 
remaining regimen time, using the following 
format: 

 

dd:hh:mm:ss 

(days:hours:minutes:seconds). 
 
The block at the top right contains the current 
regimen 

Step

Cycle

Jump

 (when a regimen 

step  is  using  the  Jump  Back  to  option), 
minimum  level  (

Min

),  and  maximum  level 

(

Max

).  The  minimum  and  maximum  levels 

will be listed according to the units specified 
under the Plot Units section as the minimum 
and maximum points at which the membrane 
stretches with each cycle. 
 
The major portion of the main display window 
is taken up with a 

plot

 designed to show the 

current  state  of  a  simulated  or  running 
regimen. This plot is generated from the data 
returned  by  the  FlexLink

®

  during  regimen 

activity. When using a platform configuration 
that  uses  vacuum  to  generate  a  downward 
stretching  of the membrane  (elongation), the 
vertical 

scale 

represents 

increasing 

elongation,  vacuum  or  strain.  When  the 
selected  unit  of  display  is  kPa,  the  vertical 
scale will start at 0.0 and move upwards as an 
increasing negative pressure level, indicating 
that  a  vacuum  is  being  applied  to  the 
membrane to stretch it downwards. 
 
The scale on the x- and y-axes can be changed 
by  clicking  on  one  of  the  two  small  double-

arrow buttons at either end of the axis. The 
button with arrows pointing away from each 
other  increases  the  axis  scale,  while  the 
button  with  arrows  pointing  toward  each 
other decreases the scale. 
 
Below the plot is a 

timeline

 that displays the 

percentage  of  the  regimen  that  has  been 
completed. 
 
To the right of the plot is a set of command 
buttons which allow the user to: 

1) 

Start

 a regimen. 

2) 

Stop

 a regimen. 

3) 

Pause

 a regimen. 

4) 

Resume

 a paused regimen. 

5) 

Simulate

 a regimen. 

6) 

Reset

 the controller. 

7)   Access 

Help

8) 

Cancel

 the program. 

 

3.4  C

ONFIGURING 

U

SERS

 

 
Prior  to  creating  and  downloading  a 
regimen, a user needs to be registered. Once 
this  task  is  completed,  regimens  can  be 
personalized under the registered user name. 
 
In the 

Configure

 menu, select 

User Editor

A  new  window  will  appear  entitled 

Users

 

(Fig. 9).  
 

 

Figure 9.

  Flex Jr.

™ 

user editor

 

 
To add a user, type in a user name (must be 
at  least  4  characters)  in  the  provided  space 

Содержание Flex Jr.

Страница 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Страница 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Страница 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Страница 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Страница 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Страница 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Страница 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Страница 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Страница 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Страница 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Страница 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Страница 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Страница 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Страница 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Страница 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Страница 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Страница 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Страница 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Страница 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Страница 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Страница 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Страница 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Страница 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Страница 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Страница 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Страница 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Страница 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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