flexcell Flex Jr. Скачать руководство пользователя страница 11

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

verify  the  tubing  is  securely  fastened,  hold  the 
back  of  the  FlexLink

®

  and  apply  a  light  pulling 

force to each tubing to make sure they do not come 
loose. The ¼” O.D. blue tubing should be secure 
when  approximately  ½”  of  the  tubing  is  in  the 
port. The 

” O.D. blue tubing should be secure 

when  approximately  ¾”  of  the  tubing  is  in  the 
port. 

 
Connect  the  power  cables  (115  VAC  or  230 
VAC)  to  the  rear  power  inlet  (

FLEXLINK 

POWER

) of the FlexLink

®

 (Fig. 4). When the 

instruments are completely set up, the power 
cords  should  be  connected  to  a 

surge 

protected

 power strip and then to a 115 VAC 

or 230 VAC, 60 Hz power outlet.  
 

2.4  C

ONNECTION 

T

M

ICROSCOPY 

D

EVICES

 

 
Use the tubing adapter as shown in Figure 4 to 
connect  the  Flex  Jr.

  to  the  microscope 

device. Connect the ¼” (6.4 mm) blue tubing 
provided with the device to the front hose barb 
on the device. Then connect the open end to 
the tubing adapter.  Next, connect the ⅜” (9.5 
mm) and the ¼” (6.4 mm) quick disconnected 
tubing, already attached to the adapter, to the 
respective 

FLEX IN

 and 

FLEX OUT

 tubing 

from the rear of the Flex Jr.

.  

 

Figure 5.

  Microscope device to Flex Jr.

 tubing 

adapter 

2.5  C

ONNECTING THE 

S

YSTEM 

V

ACUUM 

L

INES

 

 
Connect the blue ⅜” (9.5 mm) vacuum inlet 
tubing to a vacuum source. A vacuum pump 
capable of producing a maximum vacuum of 
-100  kPa  and  a  flow  rate  of,  at  least,  5.7 
ft

3

/min (161 L/min) is necessary for optimal 

performance.  
 
Keep the tubing length between the vacuum 
source  and  FlexLink

®

  as  short  as  possible. 

Also,  keep  in  mind  that  some  vacuum 
systems  will  vary  in  flow  rate  and/or 
pressure  level.  To  eliminate  or  reduce  this 
effect,  use  of  a  pressure  reservoir  between 
the vacuum source and the Flex Jr.

 Tension 

FlexLink

®

 is recommended. 

 
Flexcell

®

  International  Corp.  recommends 

the  Leybold  Trivac  D8B  Pump  vacuum 
pump  (includes  the  D8B  pump  system, 
power  cable,  gas  ballast  valve  and  AR  4-8 
return filter) as a vacuum source as well as 
using  a  pressure  reservoir.  The  vacuum 
pump  and  pressure  reservoir  can  be 
purchased 

directly 

from 

Flexcell

®

 

International Corp. 
 

NOTE:    Even  with an  optimal  vacuum source, 
there will still be some system limitations due to 
airflow.  Combinations  of  high  frequencies  and 
% elongations may not be achievable due to the 
high  vacuum  level  in  the  short  period  of  time 
requested.  The  exact  output  is  dependent  on 
many  system  variables.  See  the  Addendum  on 
page  15  for  suggestions  on  maximizing  system 
performance. 

 

 
 
 
 
 

To Flex Jr. 

To microscope device 

Содержание Flex Jr.

Страница 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Страница 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Страница 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Страница 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Страница 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Страница 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Страница 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Страница 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Страница 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Страница 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Страница 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Страница 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Страница 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Страница 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Страница 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Страница 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Страница 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Страница 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Страница 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Страница 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Страница 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Страница 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Страница 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Страница 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Страница 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Страница 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Страница 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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