background image

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

and click on 

Add User

. The new name should 

appear in the 

Existing Users

 box. 

 
To delete a user, click on the appropriate user 
name  in  the 

Existing  Users

  box  so  that  it  is 

highlighted  in  blue  and  then  click  on 

Delete 

User

. The program will ask for a confirmation 

and  issue  a  warning,  click 

Yes 

to  confirm 

deletion.  The  user  and  any  of  the  user's 
defined regimens will be deleted. 
 
To exit the Users window, click on 

Close

 

3.5

 

 C

REATING 

R

EGIMENS

 

 
Before beginning, a regimen must be created 
for  downloading.  In  the 

Regimen 

menu, 

select 

Create new

. A new window will appear 

entitled 

Regimens

  with  a  list  of  the  existing 

users  and  their  regimens  (Fig.  10).  In  the 

Existing  Users

  box,  click  on  the  user  name 

under  which  the  regimen  is  to  be  created.  A 
list of current regimens created under this user 
will  appear  in  the 

Regimens  for  Selected 

User

 box, unless no regimens have been saved 

under this user, in which case, this box will be 
empty. 
 

 

Figure 10.

  Flex Jr.

™ 

regimen editor

 

 
To  create  a  new  regimen,  click  on 

Run 

Wizard

  in  the bottom  left  hand corner. This 

option will bring up a step by step process of 
creating a new regimen. Alternatively, follow 
the sequence of steps below:  

1)  Type a regimen name in the box labeled 

Regimen Name

 
2)  Choose  the  desired  waveform  shape 

under 

Shape

  by  clicking  on  the 

downward arrow and selecting an entry 
from the drop-down list. 

 
3)  Enter  the  desired 

minimum 

and 

maximum  %  elongation  for  the  plate 
membranes (0-26%) in the boxes under 

Min

 and 

Max

.  

 
4)  Enter  the  frequency  for  the  waveform 

(0.01-2 Hz) in the box under 

Freq

 
5)  If a static regimen is required, enter the 

desired  %  elongation  at  which  the 
membrane is to be held in the 

Max 

box. 

Enter  0  in  the 

Min

  box,  and  1.0  in  the 

Freq

 box (

Min

 and 

Freq

 are irrelevant 

for static regimens). 

 
6)  If a duty cycle for the waveform is to be 

defined,  enter  the  desired  value  in  the 
box under 

DC %

. The duty cycle is the 

percent of time that the waveform shape 
remains on at the high (square wave) or 
rising  (triangle)  part  of  the  waveform. 
Duty cycle only affects the triangle and 
square waves. 

 
7)  Enter  the  number  of  times  for  the 

regimen to repeat (0-65,535) in the box 
under 

Cycles

.  When  moving  out  this 

box,  the  box  underneath 

dd:hh:mm:ss

 

will  update  to  the  calculated  regimen 
time  according  to  the  set  parameters. 
Days:hours:minutes:seconds 

is 

the 

format  for  this  entry.  However,  if  the 
time  is  entered  first,  the  program  will 
calculate the number of cycles. 

 
8)  To make a regimen step (except for the 

first  step)  return  (jump  back)  to  a 
previous  regimen  step,  enter  the  return 

Содержание Flex Jr.

Страница 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Страница 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Страница 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Страница 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Страница 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Страница 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Страница 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Страница 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Страница 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Страница 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Страница 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Страница 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Страница 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Страница 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Страница 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Страница 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Страница 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Страница 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Страница 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Страница 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Страница 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Страница 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Страница 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Страница 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Страница 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Страница 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Страница 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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