background image

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

16 

4. ADDENDUMS TO THE FLEX JR.

 TENSION SYSTEM 

 

F

OR 

O

PTIMAL 

P

ERFORMANCE OF THE 

T

ENSION UNIT

 
A. 

 

Place  the  FlexLink

®

  higher  than  the 

microscope  device.  This  will  reduce  the 
amount of moisture reaching the valves in 
the system. 

 
B. 

 

When running a regimen with static steps, 
or when the system will be idle for several 
hours  once  a  regimen  has  finished,  it  is 
necessary  to  place  the  included  Water 
Trap inline between the FlexLink

®

 and the 

baseplate or microscope device. Be sure to 
place the Water Trap 

below

 the Flex Jr.

 

Tension  FlexLink

®

.  Humid  air  from  the 

incubator  tends  to  condense  within  the 
tubing  lines.  This  moisture  may  in  turn 
lead to problems with long term regimens. 
This  fluid  collection  can  be  prevented 
from  entering  the  FlexLink

®

  by  the 

insertion of the Water Trap.  

 

The Water Trap needs to be placed so that 
it  is  maintained  in  a 

vertical  position

.  It 

should be checked frequently (twice a day 
initially  while  a  regimen  is  running)  and 
should  be  emptied  when  the  Water  Trap 
becomes  half  full.  On  the  first  trial, 
monitor the Water Trap every 
15  minutes  for  the  first  two  hours  to  be 
sure that a significant amount of water is 
not  accumulating.  If  so,  there  may  be  a 
leak 

in 

the 

platform 

and/or 

gasket/BioFlex

®

  plate  assembly.  See 

“System  Troubleshooting,  Problem  1” 
(pg.  15)  to  correct  this  problem.  The 
valves and transducers may be damaged if 
moisture is allowed to accumulate.  

 
 
C. 

 

Prior  to  each  experiment,  check  the 
baseplate  tubing  at  the  exit  point  of  the 

incubator  for  any  condensed  moisture 
(Fig. 14). If water is visible in the tubing, 
it  must  be  removed  before  running 
another  experiment.  The  water  can  be 
first  removed  by  removing  the  tubing 
from  the 

FLEX  IN 

and

  FLEX  OUT

 

connections  on  the  back  of  the 
FlexLink

®

.  Then,  disconnect  the  tubing 

connections  to  the  baseplate,  and 
connect  the 

FLEX  IN

  tubing  to  the 

FLEX  OUT

  tubing  using  the  quick-

disconnect  clips.  Apply  dry  air  flow  to 
the open end of the 

FLEX IN

 tubing to 

remove  the  moisture.  You  should  feel 
the air flowing out of the open end of the 

FLEX OUT 

tubing. 

 

 

Figure 14.

  

FLEX IN

 and 

FLEX OUT

 Tubing 

at the exit port of an incubator.

 

 

D. 

 

Use  the  system  drying  filter  and  the 
shutdown 

program 

after 

every 

Содержание Flex Jr.

Страница 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Страница 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Страница 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Страница 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Страница 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Страница 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Страница 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Страница 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Страница 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Страница 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Страница 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Страница 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Страница 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Страница 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Страница 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Страница 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Страница 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Страница 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Страница 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Страница 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Страница 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Страница 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Страница 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Страница 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Страница 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Страница 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Страница 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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