F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
18
Problem 3: Programming a long regimen (1
day or more) but the software will not allow
the regimen to be saved.
Solution 3:
The software has a limit on the number of
cycles that can be entered into any given step
(65,535). If a regimen is programmed with a
step that requires more than 65,535 cycles, the
software will give an "invalid entry" error. To
run the regimen for the time required
additional steps will need to be programmed.
This will not create any breaks in the regimen.
Simply divide the total time in half (or as
many times as is necessary) and make
additional steps with the same parameters. See
section 3.5 of the manual for instructions on
programming additional steps.
Problem 4:
“
An invalid argument was
encountered" error is shown when
attempting to assign a regimen.
Solution 4:
This problem is caused when a user name
is entered into the software without any
regimens listed under his/her name. If the
assign function sees that a user has nothing to
assign an error is displayed. Simply delete the
user(s) without a regimen or make and save a
regimen under the user(s) name(s).
Problem 5: Water is accumulating quickly
in the Water Trap
Solution 5:
This problem is likely due to a poor gasket
seal on the BioFlex
®
baseplate. See the
solution to Problem 1 to correct this. Be sure
to empty the Water Trap frequently if this
problem occurs. Water accumulation in the
FlexLink
®
can damage the valves.
Problem 6: Cells are not attaching
properly to the growth surface or are
detaching during stretching
Solution 6:
This problem can be caused by plating
too many cells. Try plating with a lower cell
density—if the cells are not confluent, they
will form a matrix with the collagen (or other
matrix coating), attaching more securely to
the growth surface. Also, check with
Flexcell
®
to be sure that the best matrix
coating for attachment of the particular cell
type is being used.
Problem 7: One or more of the Flex Jr.
™
Tension FlexLinks
®
connected to the
system are not being recognized by the
software. This is usually represented by
the error message “
Cannot Connect to
Controller” when
attempting to connect a
FlexLink
®
to assign a regimen.
Solution 7:
This problem is usually due to a lack of
communication between the computer and
one or more Flex Jr.
™
Tension FlexLinks
®
.
Check the Flex Jr.
™
Tension FlexLink
®
Ethernet port connection on the computer
and the communications connection on the
back of the FlexLink
®
to be sure that these
are secure. Check the FlexLink
®
to see if the
power is ON (the FlexLink screen should
power on). If both of these conditions are
true, close and restart the software. Try
downloading the regimen again. If the
problem is not resolved, call Flexcell
®
for
further instructions
