background image

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

11 

In  the 

Regimen

  menu  of  the  Flex  Jr.

 

software, select 

Create new

. A new window 

will  appear  entitled 

Regimens

  with  a  list  of 

the  existing  users  and  their  regimens.  In  the 

Existing  Users

  box,  click  on  the  user  name 

under  which  the  regimen  is  to  be  created.  A 
list of current regimens created under this user 
will  appear  in  the 

Regimens  for  Selected 

User

 box, unless no regimens have been saved 

under this user in which case, this box will be 
empty. 
 
To  create  a  new  regimen,  click  on 

Run 

Wizard

  in  the bottom  left  hand corner. This 

option will bring up a wizard to create a new 
regimen.  Alternatively,  follow  the  sequence 
of steps below:  
 
1)  Choose 

Custom

 from the list of waveform 

shapes  under 

Shape

  by  clicking  on  the 

downward  arrow  and  selecting  this  entry 
from the drop-down list. 

 
2)  Choose  the  appropriate  file  name  under 

File

  by  clicking  on  the  downward  arrow 

and selecting an entry from the drop-down 
list 

 
3)  Type  a  regimen  name  in  the  box  labeled 

Regimen Name

 
4)  Enter  the  time  interval  in  milliseconds 

between  two  %  elongation  values  in  the 
box under 

Time

 
5)  Enter the number of times for the regimen 

to  repeat  (0-65,535)  in  the  box  under 

Cycles

.  

 
6)  When the regimen step is complete, click 

on 

Add Step

 at the bottom. Every time a 

new/edited  step  is  saved,  the  overall 
duration of the regimen will be calculated 
and displayed in the box labeled 

Duration

 

next  to  the 

Step

  number box. If the total 

duration exceeds the maximum allowable 

duration, this value will be MAXIMUM, 
and the regimen cannot be saved. Adjust 
the time and/or cycle number to decrease 
the total duration time. 

 
7)  Click on 

Save Regimen

 
8)  When  finished  with  regimen  editing, 

click on 

Close

 

3.7  A

SSIGNING 

R

EGIMENS

 

 
In  the 

Controllers

  menu  select  the 

appropriate 

FlexLink#n

,  where  “n”  is  the 

FlexLink

®

 number to which the regimen will 

be downloaded.  
 
In  the 

Regimen 

menu,  select 

Assign,

  then 

choose  the 

Platform

User

,  and 

Regimen 

from  each  drop  down  menu  (Fig.  11).  A 
message box will appear to confirm regimen 
assignment. Click 

Yes

.  

 

NOTE:    Be  sure  to  choose  the  platform  that 
properly  corresponds  to  the  setup  being  used, 
otherwise  the  %  elongation  values  will  be 
incorrect.
  
 
For example:  
 
If using the StageFlexer with a 25 mm diameter 
Loading Station

, click on SF Loading Station 

(25mm) for the choice of platforms.  
 
If  using  the  StageFlexer  Jr.  with  a  18.5  mm 
diameter  Loading  Station

,  click  on  SF  Jr. 

Loading  Station  (18.5mm)  for  the  choice  of 
platforms. 

 
If  the  download  progresses,  no  further 
messages  will  appear,  and  the 

Start

  and 

Simulate

 buttons will be enabled. If an error 

occurred in downloading the regimen to the 
FlexLink

®

,  a  message  will  appear  alerting 

the user of a problem, and the regimen will 
not be able to run. In which case, click 

Reset 

in  the  FlexLink

®

  window,  and  then  assign 

the desired regimen again. 

Содержание Flex Jr.

Страница 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Страница 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Страница 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Страница 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Страница 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Страница 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Страница 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Страница 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Страница 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Страница 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Страница 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Страница 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Страница 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Страница 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Страница 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Страница 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Страница 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Страница 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Страница 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Страница 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Страница 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Страница 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Страница 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Страница 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Страница 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Страница 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Страница 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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