background image

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

2.6  F

LEX

S

OFT

®

 

F

LEX 

J

R

.

 

V2.0

 

S

OFTWARE AND 

S

YSTEM 

P

OWER

-

UP

 

 

The  FlexSoft

®

  Flex  Jr.

  V2.0  software 

(Flexcell

®

  Copyright  Protected  Software, 

Property 

of 

Flexcell

®

 

International 

Corporation,  2730  Tucker  Street,  Suite 
200,  Burlington,  NC  27215)  has  been  pre-
installed on the hard drive.  

 

Be  sure  to  shutdown  Windows

  properly 

by using the Shut Down command activated 
by  the  Start
  button.  Failure  to  follow  the 
above  directions  can  cause  software  and 
hardware malfunctions. 

 
The computer has Windows

 10 installed on 

the  hard  drive.  All  backup  software  is 
provided  with  the  system.  Check  all 
connections  and  connect  the  surge  protector 
into an appropriate power outlet (115 VAC or 
230 VAC). Turn ON the surge protector strip 
and check that the indicator light on the strip 
is lit. If the power light does not come on, the 
surge protector has  failed.  Replace the surge 
protector  and  attempt  to  determine  what 
caused the surge protector to fail. Turn ON the 
power switches on the computer and Flex Jr.

 

Tension  FlexLink

®

.  The  system  should  then 

boot and load Windows

®

. Double click on the 

Flex  Jr.

  V2.0  icon  to  begin  using  the 

software. 
 

NOTE:    Flexcell

®

  recommends  that  the  power 

saving or screen saver features in the Windows

®

 

software are disabled. Allowing the hard drive to 
power down while an experiment is running may 
create the need to reboot the computer before data 
can  be  viewed,  or  before  a  regimen  can  be 
downloaded  again.  It  is  recommended  that  the 
monitor be put in “sleep” mode when not in use 
and  that  the  computer  be  allowed  to  run  at  full 
power during an experiment. 

 
 
 

2.7  LCD

 

D

ISPLAY ON THE 

F

LEX 

J

R

.

 

T

ENSION 

F

LEX

L

INK

®

 

 
The front of the Flex Jr.

 Tension FlexLink

®

 

box has an LCD graphic display. When the 
Flex Jr.

  Tension  FlexLink

®

  is  turned ON, 

the LCD will display the FlexLink

®

 number 

(i.e., #1 or #2) and the regimen status  (i.e., 
Idle, Ready, or Left (the time remaining, in 
minutes,  of  a  running  regimen).  When  a 
regimen  has  been  downloaded  and/or  is 
running, the LCD will display the  time left 
(in  Days:Hours:Minutes).  See  Table  1, 
below, for the display messages with various 
FlexLink

®

 statuses. 

 

Table 1.

 Messages displayed on the Flex Jr.

 

Tension FlexLink

®

 LCD. 

FlexLink Status 

LCD message 

Power ON 

#1 IDLE 
 

Regimen downloaded 

#1 READY 
 

Regimen is running  
or being simulated 

#1 TIME LEFT 
DD:HH:MM 

Regimen has stopped 

#1 READY 
 

 

2.8  U

SING 

A

DDITIONAL 

F

LEX 

J

R

.

 

T

ENSION 

F

LEX

L

INKS

®

 

 
Using 

additional 

Flex 

Jr.

 

Tension 

FlexLinks

®

  will  allow  two  different 

regimens  to  run  simultaneously.  All  tubing 
connections  made  to  the  first  FlexLink

®

 

should  be  made  exactly  the  same  with  the 
additional  FlexLink

®

.  Each  FlexLink

®

  will 

have its own separate 

FLEX IN

 and 

FLEX 

OUT

 tubing for each platform. However, the 

SYSTEM

  tubing  can  be  shared  between 

FlexLinks

®

  with  "T"  fittings  as  long  as  the 

vacuum  source  is  capable  of  running  two 
FlexLinks

®

 with the programmed regimens. 

Regimens  with  a  combination  of  high 
frequencies  and  %  elongation  levels  will 
require a more powerful vacuum source. The 
capability  of  the  vacuum  source  with  more 
than  one  FlexLink

®

  will  need  to  be  tested 

Содержание Flex Jr.

Страница 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Страница 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Страница 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Страница 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Страница 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Страница 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Страница 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Страница 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Страница 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Страница 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Страница 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Страница 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Страница 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Страница 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Страница 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Страница 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Страница 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Страница 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Страница 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Страница 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Страница 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Страница 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Страница 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Страница 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Страница 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Страница 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Страница 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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