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Bruker Daltonik GmbH

Instrument Layout

Figure 3-14 Enter the required information

Please, enter the required information. A call to Bruker Service on +49 421 2205 430,
will provide you with the Support Session Number. Press the “Submit” button and
follow the instructions to successfully connect the remote service to your

ultraflex III

.

ultraflex III User Manual, Version 1.0

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Содержание ultraflex III

Страница 1: ...ultraflex III User Manual Version 1 0 September 2006...

Страница 2: ...r Measurement Control and Laboratory Use WARNING Connecting an instrument to a power source that is not equipped with a protective earth contact creates a shock hazard for the operator and can damage...

Страница 3: ...er Daltonik GmbH Bruker Daltonik GmbH Fahrenheitstr 4 Permoserstr 15 D 28359 Bremen D 04318 Leipzig Germany Germany Phone 49 421 2205 430 Phone 49 341 2431 395 Fax 49 421 2205 370 Fax 49 341 2431 343...

Страница 4: ...Bruker Daltonik GmbH Table of Changes Version Date Changes Remarks 1 0 2006 09 25 ultraflex III User Manual First edition ultraflex III User Manual Version 1 0 iv...

Страница 5: ...YOUT 19 3 1 Schematic of the Mass Spectrometer 19 3 1 1 Instrument Controller 20 3 1 2 Rear Panel 21 3 1 3 Vacuum System 24 3 1 4 Scout MTP Ion Source 25 3 1 4 1 Pulsed Ion Extraction 26 3 1 4 2 Targe...

Страница 6: ...Operational Readiness 47 4 2 2 Moving Targets In and Out with the Manual Cartridge 47 4 3 Using the Twister optional 51 4 3 1 Turning the Instrument On or OFF 51 4 3 2 Checking for Instrument Operatio...

Страница 7: ...work in this area When the instrument is operating high DC voltages are present in addition to the 230V AC mains supply All panels must be fitted prior to operation Only under these circumstances does...

Страница 8: ...ment for measurement control and laboratory use where the electromagnetic environment is kept under control That means in such an environment transmitting devices such as mobile phones should not be u...

Страница 9: ...e than one mass selective stage in a mass spectrometer The most common form in practice is a two stage arrangement to record MS MS or MS 2 spectra The incorporated LIFT device allows acquiring full fr...

Страница 10: ...Safety Safety considerations for the ultraflex III spectrometer include ultraflex III Safety Symbols chapter 1 3 1 Manual Conventions and Symbols chapter 1 3 2 Operating Precautions chapter 1 3 3 Ele...

Страница 11: ...ade to the instrument Indicates the OFF position of the main power switch Indicates the ON position of the main power switch 1 3 2 Manual Conventions and Symbols The following graphic text formats and...

Страница 12: ...t The ultraflex III mass spectrometer contains a Class IV B pulsed ultraviolet laser 100 J at 337 nm Wear appropriate eye protection and never look directly into the laser when any of the protective c...

Страница 13: ...correct one for your laboratory and that it meets the national safety agency guidelines for the particular country of use Warning DO NOT attempt to make adjustments replacements or repairs to this ins...

Страница 14: ...conductor the green yellow lead of the line power cord is connected to true electrical ground Any interruption of the protective ground conductor inside or outside the instrument or disconnection of t...

Страница 15: ...ne cord and a mains plug suitable for use in your country The ultraflex III mass spectrometer requires approximately 2 m2 of space on a surface that can safely support the full 550 kg instrument weigh...

Страница 16: ...ive is present Opening of the container without authorized persons will void the warranty of the instrument Our service engineers will set up the instrument in customer s laboratory The surface on whi...

Страница 17: ...3 Plug the data system end of the signal cable connections into the appropriate connections Figure 2 1 4 Plug the data system end of the Remote cable into the COM 1 port on the PC 5 Turn on the AC ma...

Страница 18: ...tonik GmbH Installation and Setup Trigger input digitizer Signal from the linear detector Signal from the reflector detector Figure 2 1 Connection at the rear of the PC ultraflex III User Manual Versi...

Страница 19: ...meter twister optional 2 Data system chapter 3 2 3 1 Schematic of the Mass Spectrometer Figure 3 1 shows the principle the ultraflex III mass spectrometer works P2 Target Reflector Reflector plate det...

Страница 20: ...ress Permanently lit as long as the docking procedure is in progress Target In Out 4 Error a Permanently lit Malfunction requires intervention of the user or a service technician details are shown on...

Страница 21: ...on the PC shows details flexControl b Flashing combined with Target Error Service mode or firmware update 9 Mains Permanent lit when the main circuit breaker is on Power On OFF OFF vents the instrumen...

Страница 22: ...Vacuum system exhaust 13 mm 1 2 inch plastic tubing Tygon filter mounted inside Individual facilities may have safety guidelines which require ventilation It is the responsibility of the user to adhe...

Страница 23: ...Bruker Daltonik GmbH Instrument Layout Figure 3 3 Schematic of the connections at the rear ultraflex III User Manual Version 1 0 23...

Страница 24: ...nd invisible light could reach your eye causing irreparable injuries 3 1 3 Vacuum System The vacuum system of the ultraflex consists of two high vacuum and three rough vacuum areas The vacuum lock of...

Страница 25: ...shot position 2 The vacuum lock inserts the target from atmosphere to the high vacuum 3 The ion optics consists of the positively or negatively charged MTP target plate P1 a second voltage plate P2 fo...

Страница 26: ...e ions do not pass the dense plume of matrix molecules and 2 achieving a time focusing effect provided that the correct parameters are applied Involved in the PIE process are three components of the i...

Страница 27: ...plate before the voltage was switched are exposed to a less electrical potential than the slow ones in the neighborhood of P1 For this reason the slow ones start on a higher potential than the fast o...

Страница 28: ...nsponder All the Scout MTP targets are equipped with a programmable semiconductor chip called transponder The transponder contains information from Bruker such as the geometry the part number and the...

Страница 29: ...o the Bradbury Nielsen principle the ion selector is operated as an improved Barowsky precursor ion selector to take advantage of this selection mode Just in the moment when the selected ions enter th...

Страница 30: ...n flight V alternating polarity Ion flight Ion flight PCIS Stray field Stray field D e f l e c t i o n P l a t e s HV 0 V Ion position H HV Figure 3 6 Operation principle of the precursor ion selector...

Страница 31: ...tepped down as known for segmented PSD or FAST Figure 3 7 P2 Target Reflector Reflector plate detector Linear detector Drift region P1 PCIS IS2 Ground potential P1 P1 Parents and fragments IS1 IS1 Fig...

Страница 32: ...can be switched off to allow the detection of the parent e g for calibration purposes P2 Target Reflector Reflector plate detector Linear detector Drift region PLMS P1 PCIS IS2 Ground potential P1 P1...

Страница 33: ...o field between both electrodes the ion motion is influenced in no way The particles continue moving as before however on this increased electrical potential This potential jump is necessary for phase...

Страница 34: ...ent using only one voltage segment to record a full daughter spectrum Electrode 4 Electrode 3 Electrode 2 Electrode 1 Ion Flight Direction LIFT Chamber Pulsed Acceleration Stage Acceleration Stage Gro...

Страница 35: ...d size of the used reflector detector The ultraflex III and other TOF mass spectrometers from the flex series use a gridless reflector with ion lenses Figure 3 10 Fragments that would arise inside the...

Страница 36: ...tiny channels 5 10 m length 0 5 0 8 mm which are coated inside with a semi conductive layer Every one of the microchannels works like an electron multiplier independent of the adjacent ones To obtain...

Страница 37: ...n of the linear detector 3 1 5 6 Digitizer Beginning with the laser shot the digitizer records the incoming analog signals from the detectors and converts them into digital information The digitizer c...

Страница 38: ...3 3 Remote Service Capability Customer Daltonics Service Board Remote Service http bdal webex com Figure 3 12 Operating principle of the remote service To obtain a maximum operating time the ultraflex...

Страница 39: ...ave Internet access on the Control PC of the ultraflex III An example how to obtain Remote Service in Europe 1 Select http bdal webex com Figure 3 13 appears 2 Select Join on the web page Figure 3 14...

Страница 40: ...ase enter the required information A call to Bruker Service on 49 421 2205 430 will provide you with the Support Session Number Press the Submit button and follow the instructions to successfully conn...

Страница 41: ...more than 30 000 samples cycle can be unattended processed The Twister transports target plates from an input rack to the mass spectrometer After the measurement the unit receives the target and depo...

Страница 42: ...m for the labeled targets to identify Barcode Identification has precedence over transponder identification Target plates without barcodes are identified by the IDs stored in their transponders 3 5 Ex...

Страница 43: ...nik GmbH Operation 4 OPERATION 4 1 Locating the Instrument Functions Turning the Instrument ON or OFF 4 1 1 Rear Panel Figure 4 1 Schematic of the connections at the rear ultraflex III User Manual Ver...

Страница 44: ...quisition Three BNC signal connectors trigger detector 1 and 2 for the digitizer mounted on the PC Control BNC connector to provide the video signal of the sample spot to a video capture card mounted...

Страница 45: ...y is inside or outside the instrument in the end position b OFF During the target moves in or out 3 IN PROGRESS Permanently lit as long as the docking procedure is in progress Target In Out 4 ERROR a...

Страница 46: ...ff etc It takes some time even when all parameters comply with the requirements This state is reflected simultaneously on the PC screen providing details via flexControl b Flash A subordinated malfunc...

Страница 47: ...oes not illuminate either the target is in the out position or the pressure inside the analyzer is too high for proper operation 4 2 2 Moving Targets In and Out with the Manual Cartridge To change a t...

Страница 48: ...bH Operation 2 Wait until the cover lid has reached the lower position Pay attention not to squash the fingers Figure 4 4 Open cover lid and free access to the manual cartridge ultraflex III User Manu...

Страница 49: ...Bruker Daltonik GmbH Operation 3 Open the manual cartridge by pulling the lever with a finger Figure 4 5 Opening the cover plate of the manual cartridge ultraflex III User Manual Version 1 0 49...

Страница 50: ...peration 4 Pull the lever with its target platform until it reaches the horizontal position Figure 4 6 Manual cartridge during opening Figure 4 7 Manual cartridge completely opened ultraflex III User...

Страница 51: ...the LOAD EJECT button to move the target into the measurement position inside the vacuum system 10 Wait for the READY LED to illuminate before beginning acquisition The total time from initiating the...

Страница 52: ...ing so can prevent proper operation 12 Press the TARGET IN OUT button momentarily Figure 3 2 Wait for the READY LED to illuminate before beginning acquisition The total time from initiating the target...

Страница 53: ...it is visibly clogged with dust to ensure proper instrument function The filter must be replaced Replace the filter by lifting the old filter up and out of the holder and slide in a new one Note Repl...

Страница 54: ...strument layout 19 Instrument Maintenance 53 L Laser device 24 Laser system 37 LIFT 33 M MALDI 9 Manual Conventions and Symbols 11 Moving targets in 47 52 Moving targets out 52 O Operating environment...

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