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Bruker SamplePro hr-MAS Скачать руководство пользователя страница 2

Copyright © by Bruker Corporation

All rights reserved. No part of this publication may be
reproduced, stored in a retrieval system, or transmitted, in any
form, or by any means without the prior consent of the publisher.
Product names used are trademarks or registered trademarks of
their respective holders.

This manual was written by

Gilbert Cyppel and Stanley J. Niles

© November 8, 2010: Bruker Biospin GmbH

Rheinstetten, Germany

P/N: Z31914

DWG-Nr.: Z4D11177

For further technical assistance on the SamplePro hr-MAS unit,
please do not hesitate to contact your nearest BRUKER dealer
or contact us directly at:

BRUKER BioSpin GMBH

am Silberstreifen

D-76287 Rheinstetten

Germany

Phone:

+ 49 721 5161 0

FAX:

+ 49 721 5171 01

E-mail:

[email protected]

Internet: www.bruker.com

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Содержание SamplePro hr-MAS

Страница 1: ...Version Innovation with Integrity NMR Spectroscopy SamplePro hr MAS 001 Sample Changer User Manual ...

Страница 2: ... their respective holders This manual was written by Gilbert Cyppel and Stanley J Niles November 8 2010 Bruker Biospin GmbH Rheinstetten Germany P N Z31914 DWG Nr Z4D11177 For further technical assistance on the SamplePro hr MAS unit please do not hesitate to contact your nearest BRUKER dealer or contact us directly at BRUKER BioSpin GMBH am Silberstreifen D 76287 Rheinstetten Germany Phone 49 721...

Страница 3: ...Instruction 14 3 4 Personal Protective Equipment 14 3 5 Basic Dangers 15 3 5 1 Location of the Robot ON OFF Switch 15 3 5 2 General Workplace Dangers 16 3 6 Environmental Protection 19 3 7 Signage 20 3 7 1 Warning Signs 20 4 Technical Data 21 4 1 General 21 4 2 Electrical 22 4 3 Operating Conditions 23 4 4 Rating Plate System 23 4 5 Rating Plate Robot 24 4 6 Rating Plate Exchange Unit 24 5 Design ...

Страница 4: ...Operating Guidelines 40 8 2 1 Switching On the Device 40 8 2 2 Switching Off the Device 40 9 Assembling the Tray with Rotors 41 10 Operation 45 10 1 SamplePro hr MAS Main Display 45 10 1 1 System View 46 10 1 2 Holder View 47 10 1 3 Sample Holder List 48 10 1 4 Right Window Panel 48 10 1 4 1 Container Information 48 10 1 4 2 Robot Information 50 10 1 4 3 Exchange Unit Information 50 10 1 4 4 Nitro...

Страница 5: ...Sensor Z racks 60 11 2 Cleaning the Outside of the SamplePro hr MAS and Units 61 11 2 1 After Cleaning 61 11 2 2 Other Cleaning Operations 61 11 3 Cleaning the Robot Barcode Reader 61 12 Dismantling and Disposal 63 12 1 Repair Declaration Form 63 13 EC Declaration of Conformity 65 14 Contact 67 Appendix A 69 A 1 Warning Signs 69 A 2 Figures 71 A 3 Tables 73 A 4 Glossary 75 A 5 Index 77 ...

Страница 6: ...6 Z31914_00_01 Table of Contents ...

Страница 7: ...available and adhered to 1 2 Policy Statement It is the policy of Bruker to improve products as new techniques and components become available Bruker reserves the right to change specifications at any time Every effort has been made to avoid errors in text and figure presentation in this publica tion In order to produce useful and appropriate documentation we welcome your com ments on this publica...

Страница 8: ...tion which could result in death or serious injury unless avoided CAUTION This combination of symbol and signal word indicates a possibly hazardous situation which could result in minor or slight injury unless avoided NOTICE This combination of symbol and signal word indicates a possibly hazardous situation which could result in damage to property or the environment unless avoided DANGER This comb...

Страница 9: ...red MAS samples to and from Bruker NMR instru ments to ensure they stay in continuous operation 2 1 Concept The sample changer consists of a laboratory robot SamplePro and a transfer system connecting the laboratory robot to the probe inside the NMR magnet The sample changer provides a Bruker standard interface through which the changer can be controlled from TopSpin and IconNMR software Refer to ...

Страница 10: ...nditions and Terms of Delivery and the legal regulations applicable at the time of the conclusion of the contract shall apply 2 3 Copyright This manual is protected by copyright and intended solely for internal use This manual must not be made available to third parties duplicated in any manner or form whether in whole or in part and the content must not be used and or communi cated except for int...

Страница 11: ...d rotor container inserting them into the NMR spectroscopy magnet and ejecting them after measurement back into the rotor container Intended use also includes compliance with all specifications in this manual Any use which exceeds or differs from the intended use shall be considered improper use No claims of any kind for damage will be entertained if such claims result from improper use 3 2 Owner ...

Страница 12: ...t regular intervals The owner must provide the personnel with the necessary protective equipment The owner must warrant that the device is operated by trained and authorised per sonnel as well as all other work such as transportation mounting start up the installation maintenance cleaning service repair and shutdown that is carried out on the device All personnel who work with or in the close prox...

Страница 13: ...rements 3 3 1 Qualifications i Note Only trained Bruker personnel are allowed to mount retrofit repair adjust and dis mantle the unit This manual specifies the personnel qualifications required for the different areas of work listed below Laboratory Personnel Laboratory personnel are health care professionals technicians and assistants staffing a research or health care facility where specimens ar...

Страница 14: ...ead of the laboratory Always comply with the instructions governing personal protective equipment posted in the work area WARNING Risk to life for unauthorized persons due to hazards in the danger and working areas Unauthorized persons who do not meet the requirements described here will not be familiar with the dangers in the working zone Therefore unauthorized persons face the risk of serious in...

Страница 15: ...lished by means of a risk assessment In order to minimize health hazards and avoid dangerous situations follow the safety instructions specified here as well as in the following chapters of this manual 3 5 1 Location of the Robot ON OFF Switch The robot ON OFF switch is located on the right side of the deck tray Figure 3 1 The Robot ON OFF Switch ...

Страница 16: ... Be sure to remove the ice to avoid its melting onto the unit CAUTION Accident hazard from contact with hot or cold surfaces on the unit Contact with the hot or cold surfaces of the unit may result in serious burns f Do not touch unit parts of cooled or heated units f Do not use damaged units f After removing any part of the unit allow it to cool or thaw before coming in con tact WARNING Accident ...

Страница 17: ... chemical hazard Infection contamination or other health endangerment as a result of contact with biological or chemical substances e g from broken samples f Clean the unit before maintenance work and or returning to Bruker for repair f Prepare a list of materials in which the unit came into contact with or measured f A signed confirmation of correctly carrying out cleaning disinfection is require...

Страница 18: ...of accident f Preparing an emergency plan f Enforcing standard sample handling procedures e g removing all samples from the rotor container and using sample rotors with dummy contents before mainte nance work etc f Training laboratory personnel NOTICE Material damage hazard from icing due to lack of nitrogen Material damage may result from icing caused by a lack of nitrogen flow f The nitrogen fil...

Страница 19: ... are regularly informed about potential dangers and are instructed in the safe handling of coolants Cleaning Liquids Cleaning liquids incorporating solvents contain toxic substances They must not be allowed to escape into the environment Disposal must be carried out by a specialist dis posal company CAUTION Danger to the environment from incorrect handling of pollutants Incorrect handling of pollu...

Страница 20: ...20 Z31914_00_01 Safety 3 7 Signage 3 7 1 Warning Signs Warning indicating a danger zone in work rooms Warning indicating a biological hazard Warning indicating severe hand injury ...

Страница 21: ...able 4 1 Technical Data Weight Data Height Width Depth Unit System 1800 566 650 mm Robot 750 566 650 mm Exchange Unit 300 150 350 mm Data Value Unit The highest measured workstation accepted value with consideration of a standard deviation of 1 5 dB from the measurement inaccuracy 71 5 dB A The highest value 1 meter from the vicinity of the neighboring workstation 69 5 dB A Table 4 3 Technical Dat...

Страница 22: ...omponents Data Value Unit Cooling device temperature range 16 to room temperature C Table 4 4 Cooling Device Temperature Range System Value Unit Voltage automatic voltage regulation 90 240 Volts Maximum Power Consumption 1045 Watts Table 4 5 Electrical Connection Values Components Value Unit Robot 500 Watts Exchange Unit 30 Watts Cooling Unit 150 Watts PC 320 Watts Monitor 45 Watts Table 4 6 Maxim...

Страница 23: ...em Data Value Unit Temperature range 15 to 30 C Relative humidity at 30 C maximum non condensing 10 85 Table 4 7 Operating Environment Manual Bruker Part Number Site Planning for AVANCE Systems 300 700 MHz UM Z31276 Site Planning for AVANCE Systems 750 950 MHz UM Z31686 Table 4 8 Bruker Site Planning Guides Model Manufacturer CE Approval Part Number Year of Production Variation Number Engineering ...

Страница 24: ... 4 6 Rating Plate Exchange Unit Figure 4 2 Rating Plate Exchange Unit Model Input Voltage Frequency Power Consumption CE Approval Manufacturer Serial Number Model Input Voltage Frequency Power Consumption Engineering Change Level Manufacturer Serial Number Part Number Variation Number ...

Страница 25: ... MAS Overview 1 2 4 3 2 1 The SamplePro Exchange Unit which is used to transfer rotors from rotor containers to the NMR probe and back 2 Flexible hoses which are connected between the SamplePro robotic sys tem the SamplePro Exchange Unit and the MAS probe 3 The MAS II Unit 4 The SamplePro Robotic System also called the changer device ...

Страница 26: ...ide the NMR magnet A MAS Pneumatic Control Unit which is used by SamplePro to control the spinning rate of the sample A MAS probe which is the interface between a sample and the spectrometer The samples to be measured are supplied in a special rotor container which can holdup to 48 samples The rotor containers can be uniquely identified by their barcode through an optional barcode reader The rotor...

Страница 27: ...to detect rotors and rotor containers 2 Barcode reader rotor containers 3 Pickup adapter 4 Safety door locker 5 The control unit for the cooling device 6 The control wheel for the nitrogen flow to prevent the formation of con densed water 7 ON OFF switch 8 The cooling rack for temperatures from 16 C to room temperature 9 The deck tray gas flow sensors is underneath 10 Safety door 1 2 3 5 6 7 8 4 9...

Страница 28: ...lue A user interaction is required White The system is initializing or running tasks Green The SamplePro robotic system is ready and waiting for requests Red A SamplePro hr MAS device error has occurred Further tasks cannot be performed Orange The system is running in service mode 3 4 5 7 6 8 1 Power Supply and Main Fuse 2 Rating Plates 3 N2 OUT N2 IN 4 Tray 5 Output Fuses 6 Supply for external te...

Страница 29: ...ePro hr MAS After a transfer the door is automatically unlocked 5 5 The SamplePro Exchange Unit The SamplePro Exchange Unit EU was designed to transfer 4 mm MAS rotors from one end of a flexible hose to the other An optional barcode reader can be supplied for reading barcodes on the rotors 5 5 1 Exchange Unit Back Panel 1 2 3 4 5 6 7 8 PN H116431 SSN 0001 VA 00 ECL 00 00 1 Vacuum Generator Exhaust...

Страница 30: ...ter and all valves that don t have contact with the transfer pneumatic Thus there is no need to use dry air or nitrogen Pressure range 6 8 bar Gas flow above 120 NL Gas Inlet 2 This inlet supplies only the transfer pneumatic Only dry air or nitrogen must be used This gas is usually taken from the outlet on the robot Pressure range 6 8 bar Gas flow above 60 NL CAN Connector This connector provides ...

Страница 31: ...2 connection you can select whether the MAS II unit should use a compatible mode to the old MAS unit P N H2620 for communication with the spectrometer This setting is necessary if you want to use a Topspin version prior to 3 0 When using Topspin version 3 0 or newer the old COM mode should not be used 1 Press the key Unit Setup from the main selection menu Figure 5 5 MAS Unit Setup The submenu MAS...

Страница 32: ...between a sample and the spectrometer High Resolution Magic Angle Spinning hr MAS probes are configured with a Z gradient aligned along the magic angle enabling the researcher to access a wide variety of NMR experimental techniques including gradient enhanced solvent suppression and artifact free 2D homo nuclear and hetero nuclear experiments The hr MAS probes are controlled by the MAS II control ...

Страница 33: ...ing The following symbols are affixed to the packaging material Always observe the sym bols during transport and handling Top Fragile Protect Against Moisture The arrow tips on the sign mark the top of the package They must always point upwards otherwise the content may be damaged Marks packages with fragile or sensitive contents Handle the package with care do not allow the package to fall and do...

Страница 34: ... packages Note the location of the center of gravity when lifting and trans porting Indicates the weight of packages Handle the marked package in accordance with its weight Indicates packages which are partially stackable Do not exceed the maximum load bearing capacity specified on the symbol in order to avoid damaging or destroying the content The packaging is air tight Damage to the barrier laye...

Страница 35: ...ation Note the extent of the damage on the transport documentation or the shipper s delivery note Initiate complaint procedures i Issue a complaint in respect to each defect immediately following detection Damage compensation claims can only be asserted within the applicable complaint deadlines The packaging contains components which are sensitive to an electrostatic charge Only allow packaging to...

Страница 36: ...ce Engineer Handling Packaging Materials Dispose of packaging material in accordance with the relevant applicable legal require ments and local regulations 6 4 Storage Storage of the Packages Store the packages under the following conditions Do not store outdoors Store in dry and dust free conditions Do not expose to aggressive media Protect against direct sunlight Avoid mechanical shocks Storage ...

Страница 37: ...incorrect installation and initial commissioning Errors during installation or initial commissioning may result in potentially fatal situa tions and may cause significant damage to property f Installation and initial commissioning must only be carried out by manufacturer or persons authorized by the manufacturer f Also consult the manufacturer in the case of subsequent location changes f Desist fr...

Страница 38: ...38 Z31914_00_01 Installation and Initial Commissioning ...

Страница 39: ...outes clear for emergency service vehicles 5 If warranted by the gravity of the emergency notify the responsible authorities 6 Delegate specialist personnel to rectify the fault 7 Before the restart check the device and ensure that all safety devices are installed and fully functional WARNING Danger of injury from improper operation Improper operation can result in serious injury and significant d...

Страница 40: ...uld define through an SOP who should be allowed to load or unload samples from rotor containers and when The operator should work carefully and optimally use rotor ID barcode collars ID Barcode When the rotor ID barcode is dirty it should be cleaned according to established instruc tion or the barcode may be incorrectly read Contamination of Samples Reusing rotors may result in contamination of a ...

Страница 41: ...cause per sonal injury f Always open the safety door before accessing the working area of the robot f If the safety door cannot be opened stop the automation using the control soft ware IconNMR f Do not reach over the safety door to access the work area 1 Open the tray locking bar by sliding the white knob on the tray 2 Insert a rotor into the slot of the rotor handling tool and press the cap down...

Страница 42: ...nsert it cap first into the flexible nozzle 4 Insert the nozzle with the cap into the intended posi tion in the tray 5 Eject the rotor by moving the slider downward 7 When all rotors have been inserted close the tray using the locking bar white knob 8 Hold the tray by the small white lifting arms and insert the tray into the cooling rack ...

Страница 43: ...vice use a non cooled rotor container The container must be set in the last position to the right as shown in the following figure Do not forget to use the adapter plate for room temperature to fix the rotor container The adapter plate must be inserted before inserting the tray The plate must be inserted so that the text FRONT is in the front Figure 9 1 Adapter Plate for Room Temperature in Last P...

Страница 44: ...44 Z31914_00_01 Assembling the Tray with Rotors ...

Страница 45: ...lePro hr MAS This chapter will describe the application control software in detail The next section describes the user interfaces for the SamplePro hr MAS if you are already familiar with this program you may proceed to the Basic Commands on page 53 i Note All panels and shields must be in place before operating this device 10 1 SamplePro hr MAS Main Display The menu driven application control sof...

Страница 46: ...port of the sample between the SamplePro hr MAS system and the probe in the magnet The system view also indicates the current status of the safety door Figure 10 1 The Main Window of the Control Software SamplePro hr MAS 2 3 4 The four positions indicated in the figure above are the positions where the rotor could be present during transport This information is then shown in the current location f...

Страница 47: ...ased on what has been read by the container barcode reader optionally or detected by the object detector Figure 10 2 Holder View The information about the selected area is displayed on the right information panel by selecting an area by clicking on it with a mouse 2 3 1 Deck Tray 2 Rotor Container 3 Rotor Position 1 ...

Страница 48: ...Window Panel The window panel on the right side of the main window provides information about the current system status including Container Information Robot Information Exchange Unit Information Nitrogen Air Flow Information Cooling Temperature Information 10 1 4 1 Container Information A container is an object that can be one of three possible types Rotor Rotor container Deck tray ...

Страница 49: ...r Displaying Rotor Container Information To display information about the rotor container in the container information panel click on the rotor container i e the blue area in the figure below Displaying Deck Tray Information To display information about the deck tray in the container information section click on the corresponding deck tray area Figure 10 5 Container Information Deck Tray ...

Страница 50: ...de Reader A Ready status by the rotor container barcode reader indicates that the barcode scan ner is initialized and ready for scanning A Not Used status indicates that the barcode reader is either not available or not ready for use 10 1 4 3 Exchange Unit Information The Exchange Unit Information panel displays the status of the SamplePro Exchange Unit and the optional rotor barcode reader In bot...

Страница 51: ...t flow field border will turn to red These preset values must be entered by a trained service representative 10 1 4 5 Cooling Temperature Information The Cooling Temperature Information panel display the target and current temperatures of the selected rotor container as well as the rotor container ID Figure 10 9 Cooling Device Information The value fields are color coded when the temperature value...

Страница 52: ...ker when problems occur Service and Settings Used to open the service window for service personnel only Exit Used to exit the program Changer Insert Sample Used to begin the insert sample procedure Eject Sample Used to begin the eject sample procedure View Container Information Used to toggle the container information on and off on the right panel of the main screen Zoom In Used in the holder view...

Страница 53: ... When the transfer is complete the status will change to Ready When a transfer procedure begins the system immediately locks the door whereas the lock is graphically displayed in the System View Figure 10 10Door Locked The safety door will be unlocked when the transfer is complete Note During the transfer procedure the current position of the rotor can be viewed in the System View As the rotor pro...

Страница 54: ...ailable sample to be transferred by clicking on the sample position with your mouse The dialog window will close and the transfer will start immediately Note When a sample is already in the probe the system will automatically perform an Eject Sample and remove the old rotor In this case the new rotor will automati cally be inserted into the probe when the old sample has been ejected When the trans...

Страница 55: ... the four positions in the System View Figure 10 1 will all be blank 10 3 Exchanging Rotors and rotor containers Before opening the safety cover to exchange rotors or rotor containers make sure that a transfer is not in progress and the status bars are showing a Ready status Note After the safety cover is opened then closed the rotor deck tray of the container will be scanned and all information w...

Страница 56: ...ry to return the sample to its ori gin When this position is occupied by another sample an error message will be dis played and the sample will be transported to the Security Eject Position 10 4 Activity History The SamplePro hr MAS program offers an activity history that can be used for example by the laboratory manager to monitor user activities or troubleshooting 1 To open the activity history ...

Страница 57: ...57 Z31914_00_01 Operation Figure 10 14Activity History ...

Страница 58: ...58 Z31914_00_01 Operation ...

Страница 59: ...m electrical shock A life threatening shock may result when the door to the housing is open during oper ation f Disconnect the device from the electrical power supply before opening the device for maintenance Use a voltmeter to verify that the device is not under power f Be sure that the power supply cannot be reconnected without notice f The housing must be closed during operation f Ensure that a...

Страница 60: ...to clean and decontaminate the instrument e g deck tips deck trays racks 11 1 2 Weekly Maintenance Weekly maintenance is performed by the operator once a week in addition to the daily maintenance 1 Check the position of the first rotor position when inserting and ejecting a rotor 2 Check the object sensor position when inserting and ejecting a rotor 11 1 3 Cleaning and Lubrication The following pa...

Страница 61: ...mination from a bro ken rotor BRUKER accepts no responsibility for any damage which may occur when samples are used containing radioactive or other hazardous materials In case of an accident with toxic radioactive explosive or biologically active substances the probe and associated equipment must be cleaned in such a way that there is no lon ger any danger in any form especially for all uninformed...

Страница 62: ...62 Z31914_00_01 Preventative Maintenance ...

Страница 63: ...tling and Disposal 12 1 Repair Declaration Form A Safety and Repair Declaration Form must be filled out whenever a probe or other unit situated in a magnet room are exposed to hazardous substances and must be returned to Bruker ...

Страница 64: ...ntamination security measures etc The customer is finally liable for all other direct and or indirect damages caused to Bruker by the hazardous substances I ACCEPT THIS OBLIGATION The repair declaration completed and signed by the safety representative has to be attached to the returned product The declaration must be attached to the delivery note on the package exterior Any returned product witho...

Страница 65: ...65 Z31914_00_01 13 EC Declaration of Conformity ...

Страница 66: ...66 Z31914_00_01 EC Declaration of Conformity ...

Страница 67: ...tact our NMR service centers Bruker BioSpin NMR provide dedicated hotlines and service centers so that our special ists can respond as quickly as possible to all your service requests applications ques tions software or technical needs Please select the NMR service center or hotline you wish to contact from our list avail able at http www bruker biospin com hotlines_nmr html ...

Страница 68: ...68 Z31914_00_01 Contact ...

Страница 69: ...ld be made at regular intervals to help detect possible vibration and or electromagnetic interference 18 To avoid mix up of the contents in rotors proper standard laboratory operating pro cedures must be followed 19 Notice Material damage hazard due to improper maintenance 17 59 Material damage hazard from icing due to lack of nitrogen 18 Material damage hazard from overflow of cryogens 16 Materia...

Страница 70: ...70 Z31914_00_01 Warning Signs ...

Страница 71: ...Position to the Right 43 Figure 10 1 The Main Window of the Control Software SamplePro hr MAS 46 Figure 10 2 Holder View 47 Figure 10 3 Sample Holder List 48 Figure 10 4 Container Information Rotor 49 Figure 10 5 Container Information Deck Tray 49 Figure 10 6 Robot Information about the Safety Door and Rotor Container Barcode Reader 50 Figure 10 7 SamplePro Exchange Unit Information 50 Figure 10 8...

Страница 72: ...72 Z31914_00_01 Figures ...

Страница 73: ... Dimensions 21 Table 4 3 Technical Data Noise Level 21 Table 4 4 Cooling Device Temperature Range 22 Table 4 5 Electrical Connection Values 22 Table 4 6 Maximum Power Consumption 22 Table 4 7 Operating Environment 23 Table 4 8 Bruker Site Planning Guides 23 Table 5 1 Exchange Unit Back Panel 30 ...

Страница 74: ...74 Z31914_00_01 Tables ...

Страница 75: ...xchange Unit GLP Good Laboratory Practices hr MAS High Resoluton Magic Angle Spinning Icon NMR Bruker software package for automation MAS Magic Angle Spinning NMR Nuclear Magnetic Resonance SamplePro Bruker name for the Sias Xantus robot XANTUS and XANTUS Junior SOP Standard Operating Procedure ...

Страница 76: ...76 Z31914_00_01 Glossary ...

Страница 77: ...61 Control software 46 Coolants 19 Copyright 10 Customer service 10 D Dangerous situations 39 Deck tray 48 Decontamination 60 Door lock 29 E Eject Sample 54 55 Emergency Shutdown 39 Exchange rotors 55 Exchange Unit 25 Automation mode 50 Description 29 Information panel 50 G Gas Inlet 30 H Helium 19 Holder View 47 I Icon NMR 9 45 IconNMR 9 45 Initial commissioning 37 Installation 33 37 ...

Страница 78: ...al Symbols 33 Personal protective equipment 14 Policy Statement 7 Power Supply Connector 30 Preventative Maintenance 59 Probes 32 Protective equipment 14 R Rating Plate 24 Relative humidity 36 Restart 39 Risk assessment 12 Robotic System 25 Components 27 Rotor 48 Rotor container 48 S Sample Holder List 48 Site Planning 23 Standard Operating Procedure 12 Storage temperature 36 System View 53 T Term...

Страница 79: ...79 Z31914_00_01 Index Transport damage 35 U Untrained personnel 10 V Vacuum Generator Exhaust 30 Ventilation Valve 30 W warranty 10 ...

Страница 80: ...80 Z31914_00_01 Index ...

Страница 81: ......

Страница 82: ...r you the opportunity to collaborate with us on new developments Our ongoing efforts and considerable investment in research and development domonstrates our long term commitment to technological innovation on behalf of our customers With more than 50 years of experience in meeting the needs of the scientific community across a range of disciplines Bruker has built a sound rapport with professiona...

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