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Page 58                                 User manual 5448X000                              Version 1 June-06

Peak-picking

On the main menu of spectrum scan-

ning, press 

(F2)

key to display peak-pic-

king operating page as shown in picture 25.

At the bottom of this page, enter the

minimum height value of picked
peaks/valleys by pressing the numeric
keys 

(0~9)

(.) 

(4.2) .  

Ce

key (4.3c) is used

to clear previous data. Press 

Enter key

(4.3d) to confirm the numeric entry and
quit. If nothing is to be entered, press

Return

key (4.3e) to quit directly. The

program permits setting a 4-digits num-

ber. In case the number you set exceeds four digits, the system will delete what has been
entered. You should start a new entry. 

After the minimum peak/valley value is entered, the system will start peak-picking cal-

culation and the calculated result will be displayed on the screen. The peaks and valleys
on the spectrum are marked. At the same time, an arrow indicates the current peak/valley
point. Below the spectrum, the wavelength and the measured value of the pointed
peak/valley are displayed. By pressing 

 

cursor keys (4.3b), the arrow will be shifted

to look up other peak/valley points. Press 

(F4)

key to print out the result. Press 

Return

key

(4.3e) to return to the main menu of the spectrum scanning after all the operations are
finished.

     Printing measuring results

When spectrum scanning is finished,

press

(F4)

key to print out the measured

spectrum.

(F4)

key will not be effective if

there is no spectrum stored. 

Press 

Enter 

key (4.3d) to print the

spectrum or press 

Ce

key (4.3c) to quit

printing.

Quitting spectrum scanning measurement

When spectrum scanning measurement operation is finished, press 

Return

key (4.3e)  to

quit spectrum scanning. The system alerts data will be lost. Users can press 

Return

key

(4.3e) to return to the main menu of the instrument.

Quantitative Measurement

In this mode one-wavelength determination can be done. Both K-Factor method and

calibration curve method are used in quantitative measurement.

14:57

Input Percent:

(1 - 100): 5

PARM

(F1)

PEAK

(F2)

PRIN

(F4)

Spectrum

Abs

200

250

300

350

400

-0.010

-0.005

0.000

0.005

0.010

660.0nm

0.000

Picture 25

14:57

Print OK?

Yes: Enter , No: CLS

PARM

(F1)

PEAK

(F2)

PRIN

(F4)

Spectrum

Abs

200

250

300

350

400

-0.010

-0.005

0.000

0.005

0.010

660.0nm

0.000

Picture 26

Version 1 June-06

User manual 5448X000                              Page 55

Parameter setting

On the main menu of spectrum scan-

ning, press 

(F1)

key to display the para-

meter setting page, as shown in picture
19.

On this page, users can set the photo-

metric mode, scanning speed, scanning
wavelength range, ordinate full scale
range and sampling interval by pressing
the corresponding numeric keys. After
parameter setting is finished, press

Return

key (4.3e) to return to the pre-

vious page.

Setting photometric mode

Press 

(1)

key to set photometric mode. Four modes are available: Abs, Er, Es and %T ,

which can be selected in turn when 

(1)

key is pressed. 

Es

and 

Er

are the energy measuring

on the reference beam and the sample beam respectively. When measuring the energy of
the light beam, the energy gain will be asked to input first , and then the system executes
a spectrum scan.

Setting scanning speed

Press 

(2)

key to set the scanning speed to fast, middle or slow in turn.

Setting sampling interval

Press 

(3)

key to set sampling interval. You have 5 choices: 1nm, 2nm, 0.1nm, 0.2nm or

0.5nm, which can be selected in turn when 

(3)

key is pressed.

A maximum of 1100 points can be stored for one spectrum, because the memory space

of microprocessor is limited. 

If the set wavelength range is wider than 110nm and the sampling interval is set to

0.1nm, the system will change it to 0.2nm automatically. In other circumstances, the set-
ting will not be changed.

Setting wavelength range

Press 

(4)

key to display the wave-

length range setting page of spectrum
scanning. The system asks the user to set
wavelength values, as shown in picture
20.

14:57

Spectrum

Parameter

1. Mode: 

Abs

2. Scan Speed: Middle

3. Interval: 

1.00

4. Scan WL:  400.0 200.0

5. Range: 

0.010 -0.010

Select Item No.:

Check

(F5)

Picture 19

14:57

Spectrum

Parameter

1. Mode: 

Abs

2. Scan Speed: Middle
3. Interval: 

1.00

4. Scan WL:  400.0 200.0
5. Range: 

0.010 -0.010

Input Scan Start WL:

(1100 - 190): 400

Check

(F5)

Picture 20

ENGLISH

ENGLISH

Summary of Contents for UV-VIS 4481

Page 1: ...be available for all users of these equipments To get the best results and a higher duration of this equipment it is advisable to read carefully this manual and follow the processes of use Ce manuel...

Page 2: ...ado provocar la p rdida total de la garant a Los fusibles o accesorios as como la p rdida de los mismos no est n cubiertos por dicha garant a Tampoco estar n cubiertos por el periodo de garant a las p...

Page 3: ...Anomalie du conver tisseur AD Ouvrez le comparti ment des chantillons Ouvrez le porte lampes D2 Remplacer le fusible de la lampe D2 Enlever l objet opa que Changer la lampe D2 Revisi n 1 Junio 06 Manu...

Page 4: ...ffent pendant le fonctionnement donc il faut s assurer le temps suffisant pour laissez refroidir les lampes avant sa remplacement Le miroir r fl chissant G il faut le couvrir une bourse en papier pour...

Page 5: ...alcool Ins rez la lampe de deut rium dans sa fiche Note la fen tre du faisceau de lumi re doit se trouver en face au miroir r fl chissant G AC POWER 120VA FUSE TYPE F AC 100 130 2A AC 200 240 1A MODE...

Page 6: ...nsi que des l sions des personnes br lures blessures et des dommages dans l installation lec trique Essayez que de l eau n entre pas dans le cadre des commandes bien qu il soit correcte ment isol Si d...

Page 7: ...en el plazo establecido Guarde el embalaje ya que siempre se deben realizar las devoluciones en su embalaje original con todos los accesorios suministrados Compruebe los accesorios que usted debe rec...

Page 8: ...ora mediante el bot n de encendido 2 4 La inicializaci n se mostrar en la pantalla de LCD 1 2 El instrumento empieza un autochequeo y se inicializa Este proceso puede durar unos 3 minu tos Despu s de...

Page 9: ...se atempere antes de comenzar a trabajar Realizar la comprobaci n en la instalaci n Antes de acabar con la instalaci n comprobar cada uno de los siguientes puntos Peri dicamente haga una revisi n en...

Page 10: ...ions n cessaires Il est possible d introduire un num ro 5 digits sur une gamme depuis 9999 9999 Le syst me enl vera toute entr e num rique sup rieure 5 digits en per mettant une nouvelle entr e 8 2 4...

Page 11: ...ein Parameter Means 1 2 Custom 1 WL A1 A2 260 0 230 0 2 Df Df1 Df2 49 10 3 840 3 Pf Pf1 Pf2 75 80 183 0 4 320 nm Adj No 5 Adj Factor 1 000 14 57 Input DNA Factor 9999 9999 DNA Protein Parameter Means...

Page 12: ...90 150 PARM F1 CLS F2 SAMP F3 PRIN F4 Photo 660 0 0 000 No Abs K Abs Figura 12 Revision 1 Juin 06 Manuel d utilisation 5448X000 Page 101 mence le mesurage des nombres d chantillons fix s 7 5 2 Les val...

Page 13: ...ager 500 groupes de mesures Le processus de mesure et les r sultats d pendent du r glage effectu dans la section 7 5 1 Lorsque la correction du blanc dans la cuve 1 est r gl e NON le syst me com 14 57...

Page 14: ...K Abs Figura 15 Revision 1 Juin 06 Manuel d utilisation 5448X000 Page 99 7 2 6 Sortie du r glage de param tre de mesure quantitative Poussez Return pour sortir du r glage de param tres de mesure quan...

Page 15: ...tion Curve Select Item No 14 57 Abs Conc 0 1 Abs R 1 000 Print F4 mg L 0 05 0 10 0 15 0 20 0 00 0 05 0 15 0 20 Revisi n 1 Junio 06 Manual de instrucciones 5448X000 P g 15 Ajuste de medida de la longit...

Page 16: ...page de r glage de param tre de mesure quantitative 7 2 5 R glage de param tres de la courbe d talonnage pour la m thode de courbe d talonnage Dans la page de r glage de param tres du mode mesure qua...

Page 17: ...9999 9999 PARM F1 14 57 Quantitation K Factor Method Formula C K Abs B 1 Slope K 4555 2 Intercept B 3400 Please Input B 9999 9999 PARM F1 Revisi n 1 Junio 06 Manual de instrucciones 5448X000 P g 17 A...

Page 18: ...2 Scan Speed Middle 3 Interval 1 00 4 Scan WL 400 0 200 0 5 Range 0 010 0 010 Input Upper Limit 9999 9999 0 002 Check F5 Figura 21 14 57 Spectrum Parameter 1 Mode Abs 2 Scan Speed Middle 3 Interval 1...

Page 19: ...int OK Yes Enter No CLS PARM F1 PEAK F2 PRIN F4 Spectrum Abs 200 250 300 350 400 0 010 0 005 0 000 0 005 0 010 660 0nm 0 000 Revisi n 1 Junio 06 Manual de instrucciones 5448X000 P g 19 Al fondo de la...

Page 20: ...00 250 300 350 400 0 010 0 005 0 000 0 005 0 010 660 0nm 0 000 Figura 25 14 57 Print OK Yes Enter No CLS PARM F1 PEAK F2 PRIN F4 Spectrum Abs 200 250 300 350 400 0 010 0 005 0 000 0 005 0 010 660 0nm...

Page 21: ...per met de confirmer l entr e num rique et Return de sortir Poussez nouveau Return s il n y a pas d autres modifications n cessaires Il est possible d introduire un num ro 4 digits 14 57 Spectrum Para...

Page 22: ...660 0 3 Conc Unit mg l Please Input WL 1100 190 PARM F1 Figura 29 14 57 Quantitation K Factor Method Formula C K Abs B 1 Slope K 4555 2 Intercept B 3400 Select Item No PARM F1 Figura 30 Revision 1 Ju...

Page 23: ...calibraci n pulsando las teclas 0 9 4 2 Ce 4 3c es usada para eliminar datos anteriores Presionando Enter 4 3d se confirma la entrada y sale de la funci n Si no se introduce ninguna entrada presionar...

Page 24: ...met d talonner les mesures des chantillons contenus dans les cuves n 2 8 au respect du blanc obtenu en d duisant sa valeur La touche 3 permet de choisir la correction entre OUI ou NON 5 4 4 Changement...

Page 25: ...Cell No Revisi n 1 Junio 06 Manual de instrucciones 5448X000 P g 25 Introduzca el valor de la concentraci n presionando las teclas num ricas 0 9 4 2 Ce 4 3c es usada para eliminar datos anteriores Des...

Page 26: ...Module 8 Sample 2 Drive Cell No 1 3 Cell 1 Blank Corr No 4 Move Cell 1 5 Cell Restoration Select Item No Figura 39 Revision 1 Juin 06 Manuel d utilisation 5448X000 Page 87 5 2 2 R glage de la valeur d...

Page 27: ...la correspondiente tecla num rica Al acabar el ajuste de par metros presionar Return 4 3e para regresar a la p gina anterior Seleccionar el tipo de m dulo de muestra Presionando 1 selecciona el tipo...

Page 28: ...co x 100 Revision 1 Juin 06 Manuel d utilisation 5448X000 Page 85 La touche num rique 1 5 permet de s lectionner le mode de travail NOTE Attendez 15 30 min la stabilisation de l appareil avant d op re...

Page 29: ...5448X000 P g 29 An lisis de DNA Prote na En este modo se puede hacer el an lisis de DNA Prote na Hay tres m todos M todo 1 M todo 2 y definici n propia Pantalla de informaci n y funci n de teclas En...

Page 30: ...000 Figura 44 14 57 Input DNA Factor 9999 9999 DNA Protein Parameter Means 1 2 Custom 1 WL A1 A2 260 0 230 0 2 Df Df1 Df2 49 10 3 840 3 Pf Pf1 Pf2 75 80 183 0 4 320 nm Adj No 5 Adj Factor 1 000 Figura...

Page 31: ...factor proteico Bajo el modo de definici n propia presionar 3 para mostrar la p gina del ajuste de fac tor proteico El sistema indicar al usuario introducir el factor proteico como muestra la figura...

Page 32: ...4 4b Autozero 4 4a y Start Stop 4 4c no son usados 14 57 Select Item No Utilities 1 Lamp Chang Over WL 359 0 2 Bandwidth 2 0 3 W Lamp On 4 D2 Lamp On 5 Control Mode PC 6 Set Time 7 Correcting Dark Cur...

Page 33: ...g n el cambio de l mpara como se muestra en la figura 49 En la parte inferior de esta p gina introduzca el valor de longitud de onda deseado presionando las teclas num ricas 0 9 4 2 Ce 4 3c es usada p...

Page 34: ...os en el funcionamiento general del equipo as como lesiones a la persona quemaduras heri das y da os a la instalaci n el ctrica Procure que no entre agua en el cuadro de controles aunque ste se encue...

Page 35: ...LEAN AND DISINFECTED THEY WOULD NOT BE ALLOWED TO REPAIR BY OUR TECHNICAL SERVICE Revisi n 1 Junio 06 Manual de instrucciones 5448X000 P g 35 7 MANTENIMIENTO El espectrofot metro ultravioleta visible...

Page 36: ...l mparas como el portal mparas se calientan considerablemente durante su funcionamiento Aseg rese de apagar el equipo durante el tiempo suficiente como para la l mpara se enfr e antes de reemplazarla...

Page 37: ...despu s de que se complete cada operaci n Si no se detecta ninguna anor malidad muestra OK Si se detecta una anormalidad muestra Err y pregunta si continua la inicializaci n o no El estado del instrum...

Page 38: ...ious checks and sets many initialized parame ter values pic 54 The system gives sta tus information after each operation is completed If no abnormality is detected it displays OK If an abnormality is...

Page 39: ...ration Therefore be sure to turn off the power switch so as to cool down the lamp sufficiently before replacing it The reflective mirror 53 G should be covered with a paper bag to avoid being conta mi...

Page 40: ...O REPAIR BY OUR TECHNICAL SERVICE TO GET THE BEST RESULTS AND A HIGHER DURATION OF THE EQUIP MENT IT IS ADVISABLE TO READ THOROUGHLY THIS MANUAL BEFORE OPERATING WITH THE EQUIPMENT INDEX OF LANGUAGES...

Page 41: ...ot to spill any liquid on the control panel though it is properly insulated In case you have any doubts do please immediately unplug the spectrophotometer see Maintenance Made under the European regul...

Page 42: ...in picture 49 At the bottom of this page enter the desired wavelength value by pressing the numeric keys 0 9 4 2 Ce key 4 3c is used to clear off the previous entry Press Enter key 4 3d to confirm th...

Page 43: ...function keys F1 F4 On this page GoTo WL 4 4b Autozero 4 4a and Start Stop 4 4c keys are not to be used 14 57 Select Item No Utilities 1 Lamp Chang Over WL 359 0 2 Bandwidth 2 0 3 W Lamp On 4 D2 Lamp...

Page 44: ...fining mode press 3 key to display the protein factor setting page The system will indicate users to input Protein factor as shown in picture 46 At the bottom of this page enter the desired protein fa...

Page 45: ...age 45 4 INSTALLATION SETTING UP Preliminary inspection Unwrap the spectrophotometer take off the involving plastic and take off the polispan protection in which it comes fitted Take off all the prote...

Page 46: ...User manual 5448X000 Page 67 DNA Protein Analysis In this mode DNA Protein analysis can be done There are three methods Method 1 Method 2 and Self defining Information display and function keys On th...

Page 47: ...of sample n 1 white 100 Version 1 June 06 User manual 5448X000 Page 47 Users can select a working mode by pressing the corresponding numeric key 1 5 Note You should wait 15 30 minutes for the instrum...

Page 48: ...pe Press 1 key to select sample module type There are two types available fixed cell hol der and 8 cell holder which can be alternatively selected each time 1 key is pressed Setting sample cell number...

Page 49: ...d automatically and you should start a new entry Sample Control On the main menu of photometric measurement press F3 key to display the sample control page as shown in pic ture 39 14 57 Please Input W...

Page 50: ...PRIN F4 Photo 660 0 0 000 No Abs K Abs Picture 12 Version 1 June 06 User manual 5448X000 Page 63 At the bottom of this page enter the concentration value by pressing the numeric keys 0 9 4 2 Ce key 4...

Page 51: ...ll 1 Meas Std Abs 4 Calibration Curve Please Input Std Conc 9999 9999 Picture 35 Version 1 June 06 User manual 5448X000 Page 51 Sample control On the main menu of photometric measurement press F3 key...

Page 52: ...660 0 0 000 No Abs K Abs Picture 15 Version 1 June 06 User manual 5448X000 Page 61 Setting the slope of calibration curve Press 1 key to display the slope setting page as shown in picture 31 At the b...

Page 53: ...Setting measuring wavelength Press GoTo WL key 4 4b to set measuring wavelength Please see section Setting working wavelength page 50 for details Auto zeroing 100 T calibration Press Autozero key 4 4...

Page 54: ...06 User manual 5448X000 Page 59 Information display and function keys On the instrument main menu press 3 key to select the quantitative measu rement mode The main menu of quan titative measurement wi...

Page 55: ...PEAK F2 PRIN F4 Spectrum Abs 200 250 300 350 400 0 010 0 005 0 000 0 005 0 010 660 0nm 0 000 Picture 25 14 57 Print OK Yes Enter No CLS PARM F1 PEAK F2 PRIN F4 Spectrum Abs 200 250 300 350 400 0 010...

Page 56: ...pic 23 and set the measuring wavelength for spectrum scanning 14 57 Spectrum Parameter 1 Mode Abs 2 Scan Speed Middle 3 Interval 1 00 4 Scan WL 400 0 200 0 5 Range 0 010 0 010 Input Upper Limit 9999...

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