Zeiss Colibri 7 User Manual Download

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Summary of Contents for Colibri 7

Page 1: ...Colibri 7 Operating Manual ...

Page 2: ...s reserved in the event of granting of patents or registration as a utility model All names of companies and products mentioned in this operating manual may be trademarks or registered trademarks Third party products are cited for information purposes only This does not represent approval or recommendation of these products Carl Zeiss Microscopy GmbH accepts no liability for the performance or use...

Page 3: ...P 17 3 1 Terminal diagrams 17 3 1 1 Terminal diagram for stand alone operation 17 3 1 2 Terminal diagram with PC and motorized microscope 18 3 2 Unpacking Colibri 7 19 3 3 Attaching Colibri 7 to the microscope and connecting it 19 3 4 Configuring Colibri 7 with MicroToolBox MTB 21 4 OPERATION 23 4 1 Starting operation of Colibri 7 for the first time 23 4 2 Controlling the Colibri 7 solid state lig...

Page 4: ...pe for fast acquisition 18 Fig 9 Attaching the lamp module to the microscope 19 Fig 10 Connecting the lamp module and components 20 Fig 11 MTB Configuring the connection type 21 Fig 12 MTB Configuring the lamp module type 22 Fig 13 TFT display page Colibri LEDs one LED switched on and activated for brightness adjustment 24 Fig 14 TFT display page Colibri LEDs three LEDs switched on and activated f...

Page 5: ...sed in this operating manual CAUTION This symbol indicates a potential hazard to the user CAUTION LED radiation LED risk group 3 according to DIN EN 62471 2009 when the user looks directly into the Colibri 7 light output UV radiation is emitted Any direct looking into the Colibri 7 light output is to be avoided Failure to heed this caution may result in eye damage Skin and eye irradiation is to be...

Page 6: ...epair work that is not carried out by authorized service personnel Failure to comply with this shall render all warranty claims invalid To ensure safe operation of the Colibri 7 solid state light source in connection with the microscope pay attention to the operating manual of the microscope Colibri 7 is not to be used in explosive environments Keep combustible and easily inflammable materials awa...

Page 7: ...stitute detachable power cables with power cables that do not meet the specifications Only the specified power cables should be used If it is determined that protective measures are no longer effective the instrument must be taken out of service and secured against inadvertent operation To repair the instrument contact the ZEISS service team in Germany see page 33 or your local ZEISS overseas repr...

Page 8: ...impair the instrument s performance Therefore the device must largely be protected from these influences and covered with the dust cover when not being in use Always check whether the instrument is switched off before you cover it Defective devices must not be disposed of with the household waste Dispose them of in compliance with the relevant legal requirements or return them to the manufacturer ...

Page 9: ...ed to correct the default either by repairing the device or replacing it with a new one at the manufacturer s discretion No warranty is given for defects caused by natural wear particularly of wearing parts and improper use of the device The device manufacturer shall not be liable for damage caused by misuse negligence or any other tampering with the device particularly the removal or replacement ...

Page 10: ...mission wavelengths and fixed excitation filters LED emission is individually adjustable for each LED in increments of 1 1 100 Standby mode to reduce power consumption Real time stabilization of brightness for low noise image captures Long time stabilization and performance optimization to improve the comparability of image captures Integrated and motorized switchable excitation filters Calibrated...

Page 11: ...DESCRIPTION OF THE DEVICE Colibri 7 Components overview ZEISS 02 2017 423052 7344 001 11 2 2 Components overview Fig 3 Components overview of the Colibri 7 solid state light source ...

Page 12: ...475 385 nm Solid state light source Colibri 7 type RYB UV 630 590 475 385 nm Solid state light source Colibri 7 type R G Y B UV 630 590 555 475 385 nm Solid state light source Colibri 7 type R G Y CBV UV 630 590 555 511 475 430 385 nm Ambient conditions Transport in packaging Permissible ambient temperature 40 C to 70 C Storage Permissible ambient temperature 10 C to 40 C Permissible relative air ...

Page 13: ...assification LED risk group 3 according to DIN EN 62471 2009 Solid state light source Colibri 7 type RGB UV LED risk group 3 according to DIN EN 62471 2009 Solid state light source Colibri 7 type RYB UV LED risk group 3 according to DIN EN 62471 2009 Solid state light source Colibri 7 type R G Y B UV LED risk group 3 according to DIN EN 62471 2009 Solid state light source Colibri 7 type R G Y CBV ...

Page 14: ...tched UV V B C G Y R 7 Analog off on selection button The rear of the LED Trigger Box contains the following elements Fig 5 8 Output of connection cable to Colibri 7 to Breakout connection socket Function and description of the selection buttons and trigger sockets Selection buttons Digital on The LED can only be switched on via the trigger sockets Fig 5 3 Digital off The LED can only be switched ...

Page 15: ...nm Functions of the control panel Display control element Function Description Display Status display of LEDs or of the device On off state of LEDs Current brightness value of the switched on LEDs in Highlighting of controllable LEDs Error messages LED buttons Switching one or several LEDs on or off LED on LED button lights up LED off LED does not light up Using the 7 LED buttons up to 6 LEDs can ...

Page 16: ... switched off Exit standby Press Standby button Shutter button Switching all LEDs on and off All LEDs up to 6 will be switched on or off simultaneously Brightness control of all activated LEDs button Brightness control of all activated LEDs switched on Activating all switched on LEDs for brightness control Control takes place in an adjusted ratio relative to the wavelengths of the LEDs Control Rot...

Page 17: ...r supply unit Control panel for Colibri 7 optional LED Trigger Box for Colibri 7 optional The device can be commissioned by the customer The lamp module of the Colibri 7 solid state light source is delivered completely assembled in accordance with the equipment ordered e g RGB UV variation with 4 excitation lines 3 1 Terminal diagrams 3 1 1 Terminal diagram for stand alone operation Fig 7 Terminal...

Page 18: ...am with PC and motorized microscope SVB 1 signal distribution box optional only for fast acquisition CAN USB optional CAN for control via ZEN 2 3 blue edition ZEISS software USB for control via third party software Fig 8 Terminal diagram with PC and motorized microscope for fast acquisition ...

Page 19: ...cation the clamping screw Fig 9 4 on the reflected light mount for fastening the lamp module to the microscope is a safety screw and may only be removed by ZEISS Service not a customer interface Otherwise there is a risk of eye damage upon laser coupling In case of microscope systems without laser applications proceed as follows Attach dovetail Fig 9 1 of the lamp module Fig 9 2 to the reflected l...

Page 20: ...panel Fig 10 8 with connecting cable to the Remote connection socket Fig 10 2 of the lamp module If used connect the LED Trigger Box Fig 10 9 with connecting cable to the Breakout connection socket Fig 10 3 of the lamp module If the microscope has a TFT display connect the CAN connection socket Fig 10 4 of the lamp module via the CAN cable to the microscope stand If Colibri 7 is to be controlled v...

Page 21: ...nfiguration by double clicking on the corresponding icon on the desktop Alternatively launch the program with Start All programs Carl Zeiss MTB 2011 2 x x x MTB2011 Configuration Like other fluorescence illuminators Colibri 7 will be used in the reflected light path and will be displayed in the Reflected Light Path directory Fig 11 1 of the microscope Select the Reflected Light Path folder Fig 11 ...

Page 22: ...t the name of the installed lamp module in the selection window of lamp modules Fig 12 2 Then click on Apply Fig 12 3 Finally click the current configuration e g Motorized Imager Dual Cam and click on the Write configuration to hardware button which will appear next to activate the configuration Exit the MTB program 1 2 3 Fig 12 MTB Configuring the lamp module type ...

Page 23: ... microscope Start the control software used from ZEN 2 3 blue edition or third party software If the control panel or the LED Trigger Box are connected they will be powered via Colibri 7 and are also ready for use 4 2 Controlling the Colibri 7 solid state light source Colibri 7 can be controlled in various ways depending on the available components via TFT display and selector wheel for illuminati...

Page 24: ...nges to green Set the desired brightness with the selector wheel on the microscope stand e g on front side of Axio Observer The current brightness adjustment of the LED will be displayed in percent in the button below the letter The G and Y intensity has always an identical value because the same LED is used for both excitation colors To adjust the brightness control of all LEDs switched on simult...

Page 25: ...n the stand if required Switch on the desired LED by shortly pressing the corresponding LED button see Fig 15 5 e g UV LED button The LED button lights up On the display Fig 15 1 the current brightness value is shown as a percentage to signal that the LED is switched on If required switch on further LEDs by pressing the corresponding LED buttons up to 6 LEDs simultaneously Activate the brightness ...

Page 26: ...e LEDs If an LED reaches 100 it cannot be set to a higher value The remaining LEDs can be controlled simultaneously until they have reached their maximum at 100 When decreasing the brightness the brightness of all LEDs will be decreased in reverse order To quickly switch on and off all LEDs switched on simultaneously press the button Fig 15 3 To activate deactivate the standby mode press the butto...

Page 27: ...nder If required activate the Show All option Click on the Colibri 7 icon to open the Colibri 7 control menu After opening the default mode of the control menu is Continuous Switch the LEDs on or off by activating deactivating the respective checkbox e g see Fig 17 Adjust the brightness for the selected wave length using the sliders entry fields or arrow buttons Activate the excitation filter for ...

Page 28: ... LED will be switched on or off with the adjusted brightness in parallel to camera exposure The bleaching impact on the probe will be reduced by synchronizing the exposure time of Colibri 7 with that of the camera Connect the trigger output of the camera e g Axiocam 506 using the trigger cable accessory for Axiocam order number 426557 0001 000 see operating manual Axiocam with the Increment outlet...

Page 29: ... or arrow buttons Activate the excitation filter for LED 567 by clicking on the 555 or 590 button at Excitation filter for LED 567 Select all LEDs simultaneously by clicking on Deactivate all LEDs or Activate all LEDs Click on Continuous or Gated to select the desired mode for image acquisition Image acquisition in Continuous mode in the Acquisition tab Continuous is the default setting for Colibr...

Page 30: ...n an experiment the Gated mode is typically faster than the Continuous mode Connect the trigger output of the camera using the trigger cable accessory for Axiocam order number 426557 0001 000 see operating manual Axiocam with the Increment outlet socket on Colibri 7 see Fig 4 1 In the Acquisition tab expand the Acquisition mode tool by clicking on the expander see Fig 20 Activate the checkboxes En...

Page 31: ...hardware settings into the Acquisition tab The control of Colibri 7 in the Imaging Setup tool of the Acquisition tab is analogous to the Microscope Control tool Additionally the switching states of LEDs and the image acquisition mode can be saved here by clicking the check boxes in the Include in this Setting field Fig 21 Fig 21 Colibri 7 control via Imaging Setup tool ...

Page 32: ...wer supply unit When using the device in warm and humid climatic zones pay attention to the following instructions Store the device in bright dry and well ventilated rooms with a humidity of less than 65 store particularly sensitive optical components and accessories such as objectives and eyepieces in a dry cabinet If the microscope is to be stored in closed containers for a prolonged period fung...

Page 33: ... work on the power supply may only be carried out by service technicians or specially authorized personnel If servicing is required please contact your local representative or Carl Zeiss Microscopy GmbH Carl Zeiss Promenade 10 07745 Jena Germany microscopy zeiss com www zeiss com microscopy Carl Zeiss Microscopy GmbH Königsallee 9 21 37081 Göttingen Germany ...

Page 34: ...cedures described in this manual are protected by the following patents US 6154282 A1 Semiconductor based excitation illuminator for fluorescence and phosphorescence microscopy authorized dealer Pulch Lorenz microscopy Am Untergrün 23 D 79232 March tel 07665 9272 0 fax 07665 9272 20 mail kontakt pulchlorenz de web pulchlorenz de ...

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