Zeiss Colibri 7, Operating Manual

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OPERATION
ZEISS Controlling the Colibri 7 solid-state light source Colibri 7
28 423052-7344-001 02/2017
Image acquisition in "Continuous" mode in the "Locate" tab
Continuous
is the default setting for Colibri 7 in the ZEN 2.3 (blue edition) control software.
When acquiring images with a camera mounted to the microscope system, the corresponding LEDs must
be manually switched on in the
Locate tab and switched off again after acquisition.
• Switch on all required LEDs by clicking the corresponding checkbox and set the desired brightness.
• Use the
Live
,
Continuous
or
Snap
buttons to acquire images (see Fig. 16).
• Switch the LEDs off by clicking with the mouse.
Image acquisition in "Gated" mode in the
"Locate" tab
Gated
allows the direct control of LEDs via the
trigger output of the camera.
The active LED will be switched on or off with the
adjusted brightness in parallel to camera exposure.
The bleaching impact on the probe will be reduced
by synchronizing the exposure time of Colibri 7
with that of the camera.
• Connect the trigger output of the camera (e.g.
Axiocam 506) using the trigger cable (accessory
for Axiocam, order number 426557-0001-000,
see operating manual Axiocam) with the
Increment outlet socket on Colibri 7 (see
(Fig. 4/
1
).
• In the Locate tab, expand the Camera tool by
clicking on the expander (see Fig. 18).
• Activate the checkboxes Enable for Snap and
Enable for Live
in
Trigger Control -> Trigger
Out (see Fig. 18)
• Activate the
Gated
mode in the
Colibri 7
control menu (see Fig. 17).
• Select the LEDs by clicking the corresponding
checkbox and set the desired brightness.
The LEDs will only be switched on or off
automatically during image acquisition.
• Open the reflected light shutter of the
microscope.
• Use the
Live
,
Continuous
or
Snap
buttons to
acquire images.
Fig. 18 "Locate" tab – "Camera" tool