References
Calibration Standards
Calibration Grid Slide from Micro Brightfield:
http://www.mbfbioscience.com
Fluorescent Reference Slides from Micros-
copy Education:
http://www.microscopyeducation.com
Olympus
http://www.olympusamerica.com/seg_sec-
tion/seg_home.asp
--
Olympus Microfire
color CCD
• CCD Array: 1600 x 1200 Pixels
• CCD Size: 14.8 mm diagonal
• Pixel Size: 7.4 microns square
• Architecture: Interline CCD, Progressive scan
(noninterlaced)
• Well Depth: 40,000 electrons
• Read Noise: 20 electrons rms
• Dark Current: 0.3 nA/cm2
• Dynamic Range: 60 dB
• Exposure Time Range: 500 microseconds - 60
seconds
• Gain Range: 1x - 16x
• Bit Depth: 12-bit Monochrome
Commercial anti-fade mounting media
•
ProLong Gold Antifade Mountant:
http://www.invitrogen.co.jp/products/pdf/
mp36930.pdf
•
Vectashield Antifade Mounting Medium:
http://www.vectorlabs.com/VECTASHIELD/
VECTASHIELD.html
•
VECTASHIELD HardSet Mounting Medium:
http://www.vectorlabs.com/products.details.
asp?prodID=1483
Fluorescence Microscopy Books/Papers
Allan, V., Ed., Protein Localization by Fluo-
rescence Microscopy: A Practical Approach,
Oxford University Press (1999).
Andreeff, M. and Pinkel, D., Eds., Introduction
to Fluorescence In Situ Hybridization: Prin-
ciples and Clinical Applications, John Wiley
and Sons (1999).
Herman, B., Fluorescence Microscopy,
Second Edition, BIOS Scientific Publishers
(1998).
Michalet, X., Kapanidis, A.N., Laurence, T.,
Pinaud, F., Doose, S., Pflughoefft, M. and
Weiss S., “The power and prospects of
fluorescence microscopies and spectrosco-
pies,” Annu Rev Biophys Biomolec Struct 32,
161–182 (2003).
Murphy, D.B., Fundamentals of Light Micros-
copy and Electronic Imaging, John Wiley and
Sons (2001). Molecular Probes.
Spector, D.L. and Goldman, R.D., Basic
Methods in Microscopy, Cold Spring Harbor
Laboratory Press (2005).
Yuste, R. and Konnerth, A., Eds., Imaging in
Neuroscience and Development: A Labora-
tory Manual, Cold Spring Harbor Laboratory
Press (2004).
Stereology References
Online Assistance:
http://www.mbfbioscience.com/live-support/
FAQ:
http://www.mbfbioscience.com/faqs/
Stereology References:
http://www.mbfbioscience.com/stereo-investigator-bibliography/
Chroma Filter Sets for AxioImager A1
Excitation Wheel
(1) Blank
(2) Dual Cy2/Cy3
(3) 350/50
(4) 402/15
(5) 490/20
(6) 555/25
(7) 645/30
(8) Blank-blocked
(9) Blank-blocked
(10) Blank-blocked
Emission Wheel by Camera
(1) IR filter
(2) Dual Cy2/Cy3
(3) 455/50
(4) 525/36
(5) 605/52
(6) 705/72
(7) Blank-blocked
(8) Blank-blocked
(9) Blank-blocked
(10) Blank no IR filter
Filter Cubes/Dichroics
(1) Transmitted cube with polarizer
(2) Cy2/Cy3 filter cube for viewing
through binoculars
(3) 3channel Dichroic --Camera only
(4) 4 channel Dichroic --Camera only
(5) Blank
(6) Reflected Brightfield cube
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews, Urbana IL 61801 USA
Phone: 217.244.0170 FAX: 217.244.6219
