50
XCell
TM
ATF System with C410:V3 Controller
User Guide
to accept default PV and PRV values. Default is recommended when entering new flow
rates. Follow similar procedures to change
E-Flow
SP.
It is recommended to
STOP
the
controller prior to change of flow rate. Following the change, re-START the controller. It will
start with the diaphragm in the fully exhausted position.
Filtrate or Harvest:
Start the
Filtrate (or Harvest) pump
only after XCell
TM
ATF System flow has stabilized. Stop
the
Filtrate /
Harvest pump
when stopping the XCell
TM
ATF System flow; accordingly, the
C410:V3 controller provides relay outputs on the E-BOX for activating or deactivating a
filtrate / harvest pump.
Contact Repligen Corporation for system specific instructions.
7.
Hollow Fiber Module and Diaphragm Replacement
The following is a guideline for replacing a Hollow Fiber Module (HFM) or the diaphragm
within the Diaphragm Pump. Since the procedures for replacing these parts are similar, a
generalized description is provided. Where necessary, more specific references and
descriptions will be provided.
Example 2
Replacing a HFM in Filtration Assembly connected to a pressure rated vessel:
Prerequisites: Steam is available and the HFM has to be replaced in mid run in a sterile
manner. Refer to Figure 1.
7.1
Separating the Filtration Assembly from the bioreactor:
7.1.1
STOP the C410 :V3 controller.
7.1.2
Stop the filtrate / harvest pump.
7.1.3
Disconnect Diaphragm Pump pneumatic line from the pump air inlet filter.
7.1.4
Close or disconnect compressed air and vacuum services from the P-BOX.
7.1.5
Disconnect any sensors from the Filtration Assembly to P-BOX
7.1.6
If necessary, remove the P-BOX from the proximity of the Filtration Assembly.
7.1.7
Close and disconnect the filtrate line.
7.1.7
Disconnect the Filtration Assembly from the vessel as follows:
a1. Securely close both bivalves connecting the Filtration Assembly to the vessel.
a2. Drain liquid from the connection. Optionally, purge the connection with water,
steam or some other medium.
a3. Disconnect the Filtration Assembly from vessel between the two bivalves.
a4. Remove the Filtration Assembly to an appropriate work area, i.e., a sink.
7.2
HFM removal:
7.2.1
Drain the system into a “sink”
7.2.2
Remove all sensors from the Filter Housing
7.2.3
Remove air inlet filter connected to the Diaphragm Pump and any other
connections to the XCell
TM
ATF System
