53
XCell
TM
ATF System with C410:V3 Controller
User Guide
2
Place the PL pump hemisphere (hemisphere with a nozzle on the side) with the
large opening in the upward position.
3
Place diaphragm, pointing down into the PL pump hemisphere.
Assure the
diaphragm gasket “O” ring is positioned uniformly about the periphery of the
pump “O” ring groove.
4
Mate the PA-pump hemisphere, wide opening down, with the diaphragm in PL
pump hemisphere.
Assure the diaphragm gasket “O” ring is positioned
uniformly about the periphery of the PA “O” ring groove.
5
The two hemispheres, with diaphragm in between, should be equally spaced.
6
Clamp the two hemispheres together.
7
Secure the Diaphragm Pump to the stand.
Diaphragm replacement Procedure for the XCell
TM
ATF10 System
1
The diaphragm within the Diaphragm Pump of the XCell
TM
ATF10 System
contains a short right angle bend on the diaphragm periphery which mates with
a counterpart groove in the PA pump hemisphere flange; therefore, for the
XCell
TM
ATF10 System, these two parts are assembled first. The two Diaphragm
Pump hemispheres are assembled with the diaphragm pointing into the PL
pump hemisphere.
2
Clamp the two hemispheres with the C-clamps, use the three long clamps first,
and place 120 degrees to each other about the Diaphragm Pump periphery.
3
Uniformly and sequentially tighten the clamps, so the diaphragm is compressed
evenly about the periphery of the two pump spheres.
4
Add the second set of short C-clamps as described above.
5
Add on the air inlet assembly to the Air inlet nozzle on the PA part.
6
Carefully invert the assembled pump sphere, so it stands on the long C-clamps.
7
One may place the pump onto its stand and/or proceed to assemble the entire
system.
7.6
Assembly
Fully assemble Diaphragm Pump, Filter Housing, reducer and accessories and prepare
for Pressure test. See, Pressure testing and sterilization, (section 8).
7.7
Use
Following successful pressure testing and sterilization reconnect the Filtration Assembly
to the bioreactor (section 9)
8.
Sterilization
WARNING:
These procedures refer to steam sterilization procedures. Steam is supplied at high
pressure and at high temperature, over 100ºC and can exceed 125ºC. All parts of
the Filtration Assembly are rapidly heated. Use similar precautions after removal of
a system from an autoclave. Such heated systems should only be handled by
qualified personnel taking all proper safety precautions.
8.1
Preparation of Filtration Assembly for autoclaving
