55
XCell
TM
ATF System with C410:V3 Controller
User Guide
Figure 9.
Filtration Assembly prepared for Autoclaving
8.1.5
Close all unprotected ports.
8.1.6
Depending on the size/configuration of the autoclave, the Filtration Assembly
can be placed inside the autoclave either vertically or at an angle if necessary.
Most autoclaves are not sufficiently tall for vertical placement.
8.1.7
Use caution during the procedure so not to damage attached parts or tubing.
Avoid kinking or stressing attached tubing.
8.2
Autoclave Cycle
8.2.1
The XCell
TM
ATF systems have been designed to be sterilized using an autoclave.
The XCell
TM
ATF System Filter Preparation and Autoclave Guide provides detailed
guidance on the entire procedure including the recommended filter integrity test
procedures and specifications. The features of the recommended cycle are
intended to ensure that the system will be effectively sterilized and that there is
minimal thermal stress imposed on the hollow fiber cartridge that could lead to
integrity failures.
The recommended cycle will require custom programming to incorporate a 90
minute preheat and 2-3 moderated prevac pulses to control the rate of
temperature change within the cartridge to a rate of 1 oC/min during the warm
up. Repligen recommends that during the cycle development, several
thermocouples should be positioned within the assembly to track the rate of
