49
XCell
TM
ATF System with C410:V3 Controller
User Guide
Example 1
Using an unpressurized bioreactor with an
XCell
TM
ATF6 System:
When using a bioreactor that cannot be pressurized such as a glass vessel or a disposable vessel,
i.e., SUB, the connection between the Filtration Assembly and the SUB will most likely not be an
SIP type connection shown in Figure 1. Placement of the Filtration Assembly and controller
relative to the bioreactor will, however, not change significantly.
Using a vessel that cannot be pressurized, both pressure and vacuum services are
needed. See Utility Requirements, Section 2.
Repligen Corporation offers custom, disposable connections to most commercial
SUBs. The connection procedure between the Filtration Assembly and vessel are
provided separately.
Start
1.
Connect the Filtration Assembly to bioreactor per separate instructions.
2.
Place the P-Box in proximity of the Filtration Assembly.
3.
Connect Signal cable between E-Box and P-Box.
4.
Connect E-Box to an appropriate electrical power source
5.
Connect the Air Line, Exhaust Line and Pump Line to their respective ports on the
P-BOX. Do not connect the Pump Line from the C410:V3 controller to the
Diaphragm Pump at this point.
6.
Power ON the E-Box. Wait for system to boot up and display the XCell
TM
ATF
System LOGON screen.
7.
LOGON, the main screen will be displayed. Proceed to the SET UP screen.
8.
Enter settings as in section 6.4.
Note XCell
TM
ATF System selection - Select ATF6 System. Note reactor pressure and
∆
height- enter 0 and measured difference between Diaphragm Pump clamp and
vessel liquid level in cm.
9.
Power up compressed Air and Vacuum services, confirm pressure and vacuum
services are on by observing the respective pressure gauges.
10.
Connect pneumatic Pump Line to Diaphragm Pump air filter.
11.
Activate the P2 trend on the MAIN screen. (Any setting adjustments in the P2
screen are made from the P2 TREND screen).
12.
Press the START button on the MAIN screen.
13.
XCell
TM
ATF System should begin cycling normally.
14.
Observe for conditions described at the end of the last section.
Note the air purge from the system after start of XCell
TM
ATF System. Note also the
small up and down change in vessel liquid level, indicating XCell
TM
ATF System cyclic
flow.
Changing flow rate:
To change flow rates, simply go to the main screen select
P-Flow
SP
. Enter the new flow
rate on the pup-up menu. Press
Yes
to accept current PV and PRV values, or press
Default
