B.13 Sample manager - flow through needle
recommendations
• Do not use concentrations of THF or hexane greater than 10% as purge solvent.
• Typical organic sample diluents such as dimethylsolfoxide (DMSO) and dimethylformamide
(DMF) are supported.
Notice:
• Do not leave buffers stored in the system.
• Flush all flow paths, including the needle wash, with plenty of non-buffered solvent
before shutting down the system.
• For extended shutdown periods (longer than 24 hours), use 10% to 20% methanol
in water.
• When using a buffered wash solvent, prime it for a minimum of 30 sec.
• Use of buffers can cause salt build-up on the needle and wash port, which can
require periodic cleaning.
B.14 Common solvent properties
The following table lists the properties for some common chromatographic solvents.
Table B–1: Properties of common solvents
Solvent
Vapor pressure mm Hg (Torr) Boiling point
(°C)
Flash point
(°C)
Acetone
184.5 at 20 °C
56.29
-20
Acetonitrile
88.8 at 25 °C
81.6
6
n
-butyl acetate
7.8 at 20 °C
126.11
22
n
-butyl alcohol
4.4 at 20 °C
117.5
37
n
-butyl chloride
80.1 at 20 °C
78.44
-9
Chlorobenzene
8.8 at 20 °C
131.69
28
Chloroform
158.4 at 20 °C
61.15
Cyclohexane
77.5 at 20 °C
80.72
-20
Cyclopentane
400 at 20 °C
49.26
-7
o
-Dichlorobenzene
1.2 at 20 °C
180.48
66
Dichloromethane
350 at 20 °C
39.75
Dimethyl acetamide
1.3 at 25 °C
166.1
70
August 8, 2016, 715005049 Rev. C
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