To start up the system:
1.
From the system view of the console, click
Control
>
Start up system
.
Alternative:
Right-click in the control panel and click
Start up system
.
2.
On the
Prime Solvents
tab, click the solvent manager sub-tab and if necessary, change
the value in the
Duration of Prime
field.
Notes:
• If you are priming with solvents that differ significantly from the current solvents, prime
the solvent manager for minimum of 5 minutes.
• If you are using a concentrated acid and a concentrated base, it is recommended that
the lines are not primed in succession. Instead, to reduce the risk of salt formation,
ensure the pump is flushed or primed with water prior to priming the second buffer.
• If you want to return settings to their original values on any tab, click
Set Defaults
.
3.
On the
Prime Solvents
tab, click the sample manager sub-tab and if necessary, change
the settings for the wash and purge solvent.
Table 3–12: Sample manager priming parameter values
Parameter
Wash solvent
Purge solvent
Priming range
1 to 600
seconds
1 to 100 cycles
Note:
Each cycle takes approximately 0.5
minutes.
Default priming
15 seconds
5 cycles
Recommended priming:
dry inlet tube
180 seconds
100-µL syringe: 60 cycles
250-µL syringe: 24 cycles
500-µL syringe: 12 cycles
Recommended priming:
changing solvents
180 seconds
100-µL syringe: 50 cycles
250-µL syringe: 20 cycles
500-µL syringe: 10 cycles
4.
On the
Equilibrate to Method
tab, click each module sub-tab and if necessary, change
the settings for the flow rate, solvent composition, temperature, and lamp state to match
your requirements at equilibration.
Table 3–13: Equilibrate to Method table values
System startup
parameters
Default
Allowed values
Method initial flow rate 0.500 mL/min
0.1 to 2.0 mL/min
August 8, 2016, 715005049 Rev. C
Page 61
