1.3.1 Quaternary solvent manager
The quaternary solvent manager (QSM) is a low-pressure mixing, high-pressure pump. It
provides steady (pulse-free) solvent flow at analytical flow rates to 1 mL/min at 103,421 kPa
(1034 bar, 15,000 psi) and to 2.2 mL/min, at reduced pressures, to 53779 kPa (537 bar, 7800
psi). The QSM can pump four degassed solvents simultaneously using a gradient proportioning
valve (GPV) to dynamically create a specified composition.
Note:
An optional 6-solvent selection valve can be added to the QSM line D for increased
solvent flexibility.
1.3.2 Sample manager-flow through needle
The sample manager-flow through needle (SM-FTN) uses a direct-injection mechanism to inject
samples drawn from plates and vials onto a chromatographic column. This injection style injects
all of the aspirated sample onto the column, without any sample volume overhead. The sample
needle is part of the fluidic path and the internal surfaces are flushed by the mobile phase during
the separation. The exterior of the needle is washed in the injection port as specified in the
method. The standard injection volume range of the SM-FTN is 10 µL, however this can be
expanded up to 1 mL through the addition of optional extension loops (installed between the
sample needle and the injection valve).
The SM-FTN also features several advanced sample conditioning features such as dilution, auto-
addition, and mixing, as well as load-ahead capabilities to reduce inject-to-inject cycle times.
1.3.2.1 Wash solvent
The sample manager needle wash system is used to minimize sample carryover. It uses a single
wash solvent and this solvent does not enter the flow path of the system.
Notice:
• Do not leave buffers stored in the system.
• Flush all flow paths, including the needle wash, with plenty of non-buffered solvent
before shutting down the system.
• For extended shutdown periods (longer than 24 hours), use 10% to 20% methanol
in water.
• When using a buffered wash solvent, prime it for a minimum of 30 sec.
• Use of buffers can cause salt build-up on the needle and wash port, which can
require periodic cleaning.
Restriction:
Avoid buffered wash solvents.
August 8, 2016, 715005049 Rev. C
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