Locking cap nut
Gold-plated compression screw
Ferrule
To tighten the fitting:
1.
Loosen the cap nut from the gold compression screw.
2.
Slide the gold compression screw with the ferrule into the inlet of the column (or in-line
filter).
3.
Finger-tighten the gold compression screw into the inlet of the column (or in-line filter).
4.
Engage the column (or in-line filter), and tighten the column (or in-line filter) onto the gold
compression screw.
5.
Tighten the cap nut onto the gold compression screw.
Important:
When re-installing this fitting, examine the ferrule for any damage, and replace it, if
necessary.
2.6 Developing methods
See also:
For information about method development and validation, consult the
Auto•Blend
Plus Technology for Ion Exchange, Size Exclusion, and Reversed-phase Chromatography
documentation.
For the greatest flexibility in method development, Waters recommends configuring the system
with the column manager and optional Auxillary column managers as well as installing the
optional 6-solvent select valve in the QSM. Using the standard Auto•Blend Plus technology will
automate the preparation of any pH specific mobile phase from pure solvents for easier method
development.
2.7 Sample preparation
The use of UPLC columns may result in additional considerations when it comes to sample
preparation.
2.7.1 Particulates
Waters recommends filtering all samples with particulates through a 0.2 µm sample filter or
installing a column pre-filter. The small column frit size (0.2 µm) can become blocked more easily
than larger HPLC column frits (2.0 µm). As a result, particle-free mobile phase solvents and
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