Symbol
Definition
Electrical and electronic equipment with this symbol may contain
hazardous substances and should not be disposed of as general waste.
For compliance with the Waste Electrical and Electronic Equipment
Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct
disposal and recycling instructions.
Serial number
REF
Part number, catalog number
Audience and purpose
This guide is intended for personnel who operate and maintain the ACQUITY UPLC I-Class
Series system. The term "Series" refers to both the latest generation ("PLUS") and previous
generations of I-Class systems. It gives an overview of the system’s technology and operation.
Intended use of the ACQUITY UPLC I-Class Series system
Waters designed the ACQUITY UPLC I-Class Series system to perform chromatographic
separations. The system is for research use only and is not intended for use in diagnostic
applications.
Calibrating
To calibrate LC systems, adopt acceptable calibration methods using at least five standards to
generate a standard curve. The concentration range for standards must include the entire range
of QC samples, typical specimens, and atypical specimens.
When calibrating mass spectrometers, consult the calibration section of the operator’s guide for
the instrument you are calibrating. In cases where an overview and maintenance guide, not an
operator’s guide, accompanies the instrument, consult the system guide or the instrument’s
online Help system for calibration instructions.
Quality control
Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a
compound. If sample trays are the same or very similar, vary the location of the QC samples in
the trays. Ensure that QC sample results fall within an acceptable range, and evaluate precision
April 4, 2018, 715005704 Rev. A
Page vii
