Figure 2–10: Screwing cap nut onto fitting
#1 knurled compression fitting
#2 locking cap nut
2.8 Sample preparation
UPLC analysis places some additional restrictions on sample preparation.
2.8.1 Particulates
The small column frit size (0.2 μm) can become blocked more easily than larger HPLC column
frits (2.0 μm). Particle-free mobile phase solvents and sample solutions are therefore essential for
UPLC analysis. See the “General guidelines” for recommendations on choosing and handling
solvents.
2.8.2 Matching sample diluents
When you use the auto-dilution option on the SM-FTN, the purge solvent serves as the sample
diluent. Ensure that your sample solution is soluble and miscible in your chosen purge solvent.
Notice:
To avoid broad peaks and poor peak shape, ensure that your sample diluents
match the initial conditions of your gradient.
2.9 Solvent recommendations
For more information on recommended solvents for your system, refer to
ACQUITY UPLC H-
Class, H-Class Bio, and I-Class Series Systems Solvent Considerations
on your documentation
media, or visit
April 4, 2018, 715005704 Rev. A
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