2.6 Cycle time (between injections)
The short run time of a UPLC separation requires efficient use of the time between analyses.
The ACQUITY sample manager - flow through needle (SM-FTN) and the sample manager - fixed
loop (SM-FL) have Load Ahead and Loop Offline features to help decrease cycle time. See the
ACQUITY UPLC Console online Help for details on how to use these features.
Tip:
When using the SM-FL, you can use a larger ID needle to reduce cycle time.
2.7 Preventing leaks
Preventing leaks ensures that the system maintains adequate pressure and sample integrity
throughout the analysis.
Leaks can occur at any tubing connection, gasket, or seal, but they are most common at tubing
connections. Low-pressure leaks (on the intake side of the solvent manager’s pump) cause
solvent loss and air introduction during the intake cycle. Leaks at high-pressure fittings
(downstream of the i2Valves) can leak solvent but do not introduce air.
To prevent leaks, follow Waters’ recommendations for the proper tightening of system fittings.
Note specifically that different techniques apply to retightening fittings versus installing them for
the first time.
See also:
• The user documentation for the modules included in your system for more details on leak
detection
• The
ACQUITY UPLC Column Manager - Active and Column Manager - Auxiliary Overview
and Maintenance Guide
for details on connecting active preheaters.
2.7.1 Installation recommendations for fittings
Three types of fitting assemblies are used within the system: PEEK (polymer-based), SST
(stainless steel), and MP35N (alloy-based). When connecting tubing, heed the following
recommendations for installing and tightening fittings.
Warning:
Observe Good Laboratory Practice (GLP) at all times, particularly when
working with hazardous materials. Consult the Material Safety Data Sheets regarding
the solvents you use. Additionally, consult the safety representative for your
organization regarding its protocols for handling such materials.
Warning:
To avoid personal contamination with biologically hazardous materials, wear
clean, chemical-resistant, powder-free gloves when performing this procedure.
Requirement:
Wear clean, chemical-resistant, powder-free gloves when performing
this procedure.
April 4, 2018, 715005704 Rev. A
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