background image

13 

 

Select this option to apply a 100%T baseline correction (100%TBaseline) and a zero-
line correction (0%TBaseline) to the sample scan (S). This correction is performed with 

the raw transmission data and is thus: 

S

0%TBaseline

100%TBaseline

0%TBaseline

 

With this option, the Cary will prompt you to perform a 100%TBaseline first, followed 
by a 0%TBaseline when you perform a baseline collection. 
This baseline option corrects for any inherent variations in the electronic zero line of 
the instrument. You should use this baseline option if your samples have areas of very 
low transmission (high absorbance) as any variations in the instrument's zero line will 

affect your measurements if they you do not correct for these. You should refer to ASTM 
method E903 for further details. 

Zero SRA correction 

Select this option to apply a 100 %T baseline correction (100%TBaseline) and a zero-
line correction (0%TBaseline) to a sample (S) measured with an Absolute VW Specular 

Reflectance Accessory (SRA).  

 

Note

 

The VN SRA and the single bounce relative SRA accessories should not use this 

correction. Select a Baseline/Zero correction instead. 

This correction is performed with the raw transmission data and, due to the double 

bounce on the sample, is thus: 

S

0%TBaseline

100%TBaseline

0%TBaseline

 

With this option, the Cary will prompt you to perform a 100%TBaseline first, followed 
by a 0%TBaseline when you perform a baseline collection. You should refer to ASTM 
method E903 for further details. 

Zero *Std. Ref. Correction 

Select this option to apply a 100 %T baseline (100%TBaseline) correction and a zero-

line correction (0%TBaseline) to a sample (S) measured with a reflectance accessory 
which is not absolute (e.g., a Diffuse Reflectance Accessory or the Variable Angle 
Specular Reflectance accessory). The Cary then multiplies the result by the appropriate 

value in the 'Baseline Stdref' file which are the transmission values associated with the 
Standard Reference material you used to establish the baseline. Refer to the help for the 
Diffuse Relectance accessories for further details about Standard Reference materials. 
This correction is performed with the raw transmission data and is thus: 

S

0%TBaseline

100%TBaseline

0%TBaseline

Baseline

 

Stdref

 

Summary of Contents for Cary 5000

Page 1: ...n on computer 2 Check lens and mirror are correct for your measurement a Install Small Sample Kit mirror M3 SSK or regular M3 mirror if needed b If using SSK install correct lens L2 3 Make sure cover...

Page 2: ...e Turn on the instrument 0 I switch on the lower left side of the front of the instrument In the Scan program watch the lower left corner to see the status of the instrument When the traffic light is...

Page 3: ...mounts in front of the sample see Figure 4 There are three lenses that can be used again for the three positons marked as T C or R Each time the sample configuration is changed the optics need to be...

Page 4: ...T C and R for the three positions of the integrating sphere Also the large mirror M3 is switched out for a one with a different curvature this mirror is always kept in the T position regardless of the...

Page 5: ...on located in the upper left hand corner defines the scan parameters The following useful tabs can be found under Setup Cary tab Here you can set the units of the x and y axes nm cm 1 etc and A T R et...

Page 6: ...be applied to a sample where the abscissa range of the sample exceeds that of the baseline If you change your abscissa range to one outside that of the baseline the Cary will display an error message...

Page 7: ...x at the end of a collect where you can enter the Batch file name for your data Note In the Scan application Prompt at Start and Prompt at End save information as a DSW file unless there are multi or...

Page 8: ...multiple graphs in the Graphics area You can double click on the Graphics area to toggle the display through graph and report to graph only The graph functions can also be accessed by clicking one of...

Page 9: ...ich Text Format file How to Export Collected Data If you want to export data from a Cary WinUV application into a third party application then save your data as an ASCII CSV file To do this 1 Click Fi...

Page 10: ...eck box is not checked 8 In the File name field type in the file name for your new batch file 9 Click Save to create the new batch file The current method will be stored with the batch files All the d...

Page 11: ...SBW will change automatically to maintain the required signal level If you are collecting data in double beam mode across the UV Vis and NIR regions with Fixed SBW set as the Measurement Mode then you...

Page 12: ...nes for another Scan run you simply open the file using either the File Open Data command or click the Baseline button in the Baseline tab of the Setup dialog box Note If you decide that you do not wa...

Page 13: ...gle bounce relative SRA accessories should not use this correction Select a Baseline Zero correction instead This correction is performed with the raw transmission data and due to the double bounce on...

Page 14: ...owever sometimes you may notice that the text has turned to gray and is in italics This indicates that the Cary is currently idling outside the abscissa range of the baseline file but that the current...

Page 15: ...ferences and Graph Preferences options 1 Graph menu options Print XY point Select this to print the cursor s current X and Y coordinates in the report area This option is only available when right cli...

Page 16: ...to Arrange Graphs Select Auto Arrange Graphs to view and arrange all graphs simultaneously Copy Graph Select this to copy the Graphics area onto the Windows Clipboard Paste to Graph Select this to pas...

Page 17: ...in traces You can resize the Name of Trace and File name fields by selecting and dragging the splitter bar between the fields File Name This field displays the file name and path of the trace If the n...

Page 18: ...il the font is changed again Trace Style This field allows you to define the way that your trace will appear on screen Points Each individual data point collected by the Cary is shown on the graph Poi...

Page 19: ...e right side of each graph box A single click on a sample name or example line within the legend will make it the focused or red scan Information about the trace will be displayed below the X axis Dou...

Reviews: