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A baseline cannot be applied to a sample where the abscissa range of the sample 
exceeds that of the baseline. If you change your abscissa range to one outside that of the 

baseline, the Cary will display an error message. Also the red text in the ordinate 
display which indicates that baseline correction mode is activated will disappear. 
Not that all values within the Baseline abscissa range are corrected, even the value 
displayed while the Cary is idling at a particular wavelength. 

Note

 

 You should be careful applying corrections to samples where the following 

parameters are different in the baseline file and the samples. Energy, SBW, Beam Mode, 
Source Changeover, Detector Changeover Grating Changeover, Selected Lamp for the 

collection, Signal-to-Noise Mode, Slit height and the Independent Control.

 

Baselines are saved as a part of a batch file. However, if you want, you can also store the 

baselines by themselves in a baseline file without the collected data. To do this you 
simply select the Baseline (*.CSW) as the Save As Type in the Save As window accessed 
from the File | Save Data As menu items. When you want to use the baselines for 

another Scan run, you simply open the file using either the File | Open Data command or 
click the 

Baseline

 button in the Baseline tab of the Setup dialog box. 

 

Note

 

If you decide that you do not want to use that stored file, but want to collect a new 
baseline instead, then the Cary will use the new, collected baseline in 

all

 

corrections. However, the Baseline tab will still display the name of the baseline in 
the baseline file. You need to save the collected data as a batch file if you want the 
new baseline included in that file.  

 

Note

 

All corrections are performed with the raw transmission data that comes directly 

from the instrument. These transmission values are then converted into the 
selected Y mode for display. 

A baseline cannot be applied to a sample where the abscissa range of the sample 

exceeds that of the baseline. If you wish to use that baseline then you will need to 
reduce the range of the scan by changing the Start and Stop fields on the Cary tab of the 

Setup dialog box. 

Types of baselines that can be performed. 

Choose the type of baseline correction under the Baseline tab in the Setup menu 

Baseline Correction 

Select this option to apply a baseline correction (Baseline) to the sample scan (S) that is 

collected from the instrument as transmission values. 
This correction is thus: 

𝑆

𝐵𝑎𝑠𝑒𝑙𝑖𝑛𝑒

 

Zero/Baseline Correction 

Summary of Contents for Cary 5000

Page 1: ...n on computer 2 Check lens and mirror are correct for your measurement a Install Small Sample Kit mirror M3 SSK or regular M3 mirror if needed b If using SSK install correct lens L2 3 Make sure cover...

Page 2: ...e Turn on the instrument 0 I switch on the lower left side of the front of the instrument In the Scan program watch the lower left corner to see the status of the instrument When the traffic light is...

Page 3: ...mounts in front of the sample see Figure 4 There are three lenses that can be used again for the three positons marked as T C or R Each time the sample configuration is changed the optics need to be...

Page 4: ...T C and R for the three positions of the integrating sphere Also the large mirror M3 is switched out for a one with a different curvature this mirror is always kept in the T position regardless of the...

Page 5: ...on located in the upper left hand corner defines the scan parameters The following useful tabs can be found under Setup Cary tab Here you can set the units of the x and y axes nm cm 1 etc and A T R et...

Page 6: ...be applied to a sample where the abscissa range of the sample exceeds that of the baseline If you change your abscissa range to one outside that of the baseline the Cary will display an error message...

Page 7: ...x at the end of a collect where you can enter the Batch file name for your data Note In the Scan application Prompt at Start and Prompt at End save information as a DSW file unless there are multi or...

Page 8: ...multiple graphs in the Graphics area You can double click on the Graphics area to toggle the display through graph and report to graph only The graph functions can also be accessed by clicking one of...

Page 9: ...ich Text Format file How to Export Collected Data If you want to export data from a Cary WinUV application into a third party application then save your data as an ASCII CSV file To do this 1 Click Fi...

Page 10: ...eck box is not checked 8 In the File name field type in the file name for your new batch file 9 Click Save to create the new batch file The current method will be stored with the batch files All the d...

Page 11: ...SBW will change automatically to maintain the required signal level If you are collecting data in double beam mode across the UV Vis and NIR regions with Fixed SBW set as the Measurement Mode then you...

Page 12: ...nes for another Scan run you simply open the file using either the File Open Data command or click the Baseline button in the Baseline tab of the Setup dialog box Note If you decide that you do not wa...

Page 13: ...gle bounce relative SRA accessories should not use this correction Select a Baseline Zero correction instead This correction is performed with the raw transmission data and due to the double bounce on...

Page 14: ...owever sometimes you may notice that the text has turned to gray and is in italics This indicates that the Cary is currently idling outside the abscissa range of the baseline file but that the current...

Page 15: ...ferences and Graph Preferences options 1 Graph menu options Print XY point Select this to print the cursor s current X and Y coordinates in the report area This option is only available when right cli...

Page 16: ...to Arrange Graphs Select Auto Arrange Graphs to view and arrange all graphs simultaneously Copy Graph Select this to copy the Graphics area onto the Windows Clipboard Paste to Graph Select this to pas...

Page 17: ...in traces You can resize the Name of Trace and File name fields by selecting and dragging the splitter bar between the fields File Name This field displays the file name and path of the trace If the n...

Page 18: ...il the font is changed again Trace Style This field allows you to define the way that your trace will appear on screen Points Each individual data point collected by the Cary is shown on the graph Poi...

Page 19: ...e right side of each graph box A single click on a sample name or example line within the legend will make it the focused or red scan Information about the trace will be displayed below the X axis Dou...

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