46
Appendix A
DNA/Protein Test Algorithm
Test Name
Method
Wavelength(s)
Calculations
Parameters
Displayed
Units
DNA MEASUREMENT
Absorbance
difference
(260,280)
A
1
=A
260nm
A
2
=A
280nm
A
ref
=A
320nm
(optional)
DNA
concentration:
(A
1
-A
ref
)f
1
-(A
2
-A
ref
)f
2
Protein
concentration
(A
2
-A
ref
)f
3
-(A
1
-A
ref
)f
4
f
1
=62.9
f
2
=36.0
f
3
=1552
f
4
=757.3
Absorbance
difference
(260,230)
A
1
=A
260nm
A
2
=A
230nm
A
ref
=A
320nm
(optional)
DNA
concentration:
(A
1
-A
ref
)f
1
-(A
2
-A
ref
)f
2
Protein
concentration
(A
2
-A
ref
)f
3
-(A
1
-A
ref
)f
4
f
1
=49.1
f
2
=3.48
f
3
=183
f
4
=75.8
DNA:
μ
g/ml
Protein:
μ
g/ml
DNA/Protein
Concentration
and
DNA purity
Absorbance
ratio
A
1
=A
260nm
A
2
=A
280nm
or
A
230nm
A
ref
=A
320nm
(optional)
Ratio=
A
1
-A
ref
A
2
-A
ref
None
No units(ratio)
